Selective reduction of allelic variants

ABSTRACT

Disclosed herein are antisense compounds and methods for selectively reducing expression of an allelic variant of a huntingtin gene containing a single nucleotide polymorphism (SNP). Such methods, compounds, and composition are useful to treat, prevent, or ameliorate Huntington&#39;s Disease (HD).

SEQUENCE LISTING

The present application is being filed along with a Sequence Listing inelectronic format. The Sequence Listing is provided as a file entitledBIOL0125USC1SEQ_ST24.txt created Mar. 3, 2015, which is 344 Kb in size.The information in the electronic format of the sequence listing isincorporated herein by reference in its entirety.

FIELD OF THE INVENTION

Embodiments of the present invention provide methods, compounds, andcompositions for selectively reducing expression of an allelic variantof a gene containing a single nucleotide polymorphism (SNP). Suchmethods, compounds, and compositions are useful to treat, prevent, orameliorate Huntington's Disease.

BACKGROUND OF THE INVENTION

Genetic diseases are caused by abnormalities in genes or chromosomes.Such abnormalities may include insertions, deletions, and expansions.Huntington's Disease (HD) is one example of a genetic disease caused byan expansion. HD is a progressive neurodegenerative disorder that isinherited in a dominant fashion and results from a mutation that expandsthe polymorphic trinucleotide (CAG) tract in the huntingtin gene (HTT).The average CAG tract size in the general population is 17-26 repeats(wild type allele), however, in HD patients the CAG tract has expandedto 36 repeats or more (mutant allele) (Huntington's DiseaseCollaborative Research Group 1993. Cell 72(6):971-83). The HTT geneencodes the HTT protein and the expanded CAG tract results in apathological increase in the polyglutamine repeats near the N-terminalof the protein. Individuals carry two copies of the HTT gene and onemutant allele is sufficient to result in HD.

HTT protein appears to have a role during development of the nervoussystem and a protective role in cells. In mouse models, constitutiveknockout of the HTT gene is lethal during embryonic development (Nasiret al 1995. Cell 81(5):811-23), while adult inactivation of the HTT geneleads to progressive cell death in the brain and the testes (Dragatsiset al 2000. Nat. Genet 26:300-306). Reduction of huntingtin expressionfrom the wild type allele may, therefore, have negative consequences.

Like HD, there are disorders for which a strategy of selective reductionof a mutant allele would be beneficial. Thus, there remains an unmetneed to selectively reduce expression of mutant allelic variants likethat of HTT, which are causative of disease, over the wild type variant,which appears to be necessary for normal cellular processes.

BRIEF DESCRIPTION OF THE FIGURE

FIG. 1 provides the mRNA and genomic HTT sequence showing SNP positions.

SUMMARY OF THE INVENTION

Provided herein are methods, compounds, and compositions for selectivelyreducing expression of an allelic variant of a gene containing a singlenucleotide polymorphism (SNP). Such methods, compounds, and compositionsare useful to treat, prevent, or ameliorate Huntington's Disease (HD).SNPs may be associated with a mutant allele, the expression of whichcauses HD.

In certain embodiments, selective reduction of mRNA and proteinexpression of a mutant huntingtin is achieved by targeting a SNP locatedon the mutant allele with an antisense compound. In certain embodiments,the antisense compound is an antisense oligonucleotide

In certain embodiments, antisense compounds designed to selectivelyreduce an allelic variant of a gene containing a SNP are created basedon potency and selectivity of the antisense compound as well aspopulation genetics.

DETAILED DESCRIPTION OF THE INVENTION

It is to be understood that both the foregoing general description andthe following detailed description are exemplary and explanatory onlyand are not restrictive of the invention, as claimed. Herein, the use ofthe singular includes the plural unless specifically stated otherwise.As used herein, the use of “or” means “and/or” unless stated otherwise.Furthermore, the use of the term “including” as well as other forms,such as “includes” and “included”, is not limiting. Also, terms such as“element” or “component” encompass both elements and componentscomprising one unit and elements and components that comprise more thanone subunit, unless specifically stated otherwise.

The section headings used herein are for organizational purposes onlyand are not to be construed as limiting the subject matter described.All documents, or portions of documents, cited in this application,including, but not limited to, patents, patent applications, articles,books, and treatises, are hereby expressly incorporated by reference forthe portions of the document discussed herein, as well as in theirentirety.

DEFINITIONS

Unless specific definitions are provided, the nomenclature utilized inconnection with, and the procedures and techniques of, analyticalchemistry, synthetic organic chemistry, and medicinal and pharmaceuticalchemistry described herein are those well known and commonly used in theart. Standard techniques may be used for chemical synthesis, andchemical analysis. Where permitted, all patents, applications, publishedapplications and other publications, GENBANK Accession Numbers andassociated sequence information obtainable through databases such asNational Center for Biotechnology Information (NCBI) and other datareferred to throughout in the disclosure herein are incorporated byreference for the portions of the document discussed herein, as well asin their entirety.

Unless otherwise indicated, the following terms have the followingmeanings:

“2′-O-methoxyethyl” (also 2′-MOE and 2′-O(CH₂)₂—OCH₃) refers to anO-methoxy-ethyl modification of the 2′ position of a furosyl ring. A2′-O-methoxyethyl modified sugar is a modified sugar.

“2′-O-methoxyethyl nucleotide” means a nucleotide comprising a2′-O-methoxyethyl modified sugar moiety.

“5-methylcytosine” means a cytosine modified with a methyl groupattached to the 5′ position. A 5-methylcytosine is a modifiednucleobase.

“Active pharmaceutical agent” means the substance or substances in apharmaceutical composition that provide a therapeutic benefit whenadministered to an individual. For example, in certain embodiments anantisense oligonucleotide targeted to an allelic variant is an activepharmaceutical agent.

“Active target region” or “target region” means a region to which one ormore active antisense compounds is targeted. “Active antisensecompounds” means antisense compounds that reduce target nucleic acidlevels or protein levels.

“Administered concomitantly” refers to the co-administration of twoagents in any manner in which the pharmacological effects of both aremanifest in the patient at the same time. Concomitant administrationdoes not require that both agents be administered in a singlepharmaceutical composition, in the same dosage form, or by the sameroute of administration. The effects of both agents need not manifestthemselves at the same time. The effects need only be overlapping for aperiod of time and need not be coextensive.

“Administering” means providing a pharmaceutical agent to an individual,and includes, but is not limited to administering by a medicalprofessional and self-administering.

“Allele” is one member of a pair of genes or one member of a series ofdifferent forms of a DNA sequences that can exist at a single locus ormarker on a specific chromosome. For a diploid organism or cell or forautosomal chromosomes, each allelic pair will normally occupycorresponding positions (loci) on a pair of homologous chromosomes, oneinherited from the mother and one inherited from the father. If thesealleles are identical, the organism or cell is said to be ‘homozygous’for that allele; if they differ, the organism or cell is said to be‘heterozygous’ for that allele. “Major allele” refers to an allelecontaining the nucleotide present in a statistically significantproportion of individuals in the human population. “Minor allele” refersto an allele containing the nucleotide present in a relatively smallproportion of individuals in the human population. “Wild type allele”refers to the genotype typically not associated with disease ordysfunction of the gene product. “Mutant allele” refers to the genotypeassociated with disease or dysfunction of the gene product.

“Allelic variant” refers to one of the pair of genes or DNA sequenceexisting at a single locus. For example, an allelic variant may refer toeither the major allele or the minor allele.

“Amelioration” refers to a lessening of at least one indicator, sign, orsymptom of an associated disease, disorder, or condition. The severityof indicators may be determined by subjective or objective measures,which are known to those skilled in the art.

“Animal” refers to a human or non-human animal, including, but notlimited to, mice, rats, rabbits, dogs, cats, pigs, and non-humanprimates, including, but not limited to, monkeys and chimpanzees.

“Antibody” refers to a molecule characterized by reacting specificallywith an antigen in some way, where the antibody and the antigen are eachdefined in terms of the other. Antibody may refer to a complete antibodymolecule or any fragment or region thereof, such as the heavy chain, thelight chain, Fab region, and Fc region.

“Antisense activity” means any detectable or measurable activityattributable to the hybridization of an antisense compound to its targetnucleic acid. In certain embodiments, antisense activity is a decreasein the amount or expression of a target nucleic acid or protein encodedby such target nucleic acid.

“Antisense compound” means an oligomeric compound that is capable ofundergoing hybridization to a target nucleic acid through hydrogenbonding.

“Antisense inhibition” means reduction of target nucleic acid levels ortarget protein levels in the presence of an antisense compoundcomplementary to a target nucleic acid compared to target nucleic acidlevels or target protein levels in the absence of the antisensecompound.

“Antisense oligonucleotide” means a single-stranded oligonucleotidehaving a nucleobase sequence that permits hybridization to acorresponding region or segment of a target nucleic acid.

“Bicyclic sugar” means a furosyl ring modified by the bridging of tworing atoms. A bicyclic sugar is a modified sugar.

“Bicyclic nucleoside” means a nucleoside having a sugar moietycomprising a bridge connecting two carbon atoms of the sugar ring,thereby forming a bicyclic ring system. In certain embodiments, thebridge connects the 4′-carbon and the 2′-carbon of the sugar ring.

“Cap structure” or “terminal cap moiety” means chemical modifications,which have been incorporated at either terminus of an antisensecompound.

“cEt” or “constrained ethyl” means a bicyclic nucleoside having a sugarmoiety comprising a bridge connecting the 4′-carbon and the 2′-carbon,wherein the bridge has the formula: 4′-CH(CH₃)—O-2′.

“Chemically distinct region” refers to a region of an antisense compoundthat is in some way chemically different than another region of the sameantisense compound. For example, a region having 2′-O-methoxyethylnucleotides is chemically distinct from a region having nucleotideswithout 2′-O-methoxyethyl modifications.

“Chimeric antisense compound” means an antisense compound that has atleast two chemically distinct regions.

“Co-administration” means administration of two or more pharmaceuticalagents to an individual. The two or more pharmaceutical agents may be ina single pharmaceutical composition, or may be in separatepharmaceutical compositions. Each of the two or more pharmaceuticalagents may be administered through the same or different routes ofadministration. Co-administration encompasses parallel or sequentialadministration.

“Complementarity” means the capacity for pairing between nucleobases ofa first nucleic acid and a second nucleic acid.

“Contiguous nucleobases” means nucleobases immediately adjacent to eachother.

“Differentiating polymorphism” means a variation in a nucleotidesequence that permits differentiation between a wild type and a mutantallele of a nucleic acid sequence. Differentiating polymorphisms mayinclude insertions or deletions of one or a few nucleotides in asequence, or changes in one or a few nucleotides in a sequence. Adifferentiating polymorphism or polymorphic allele can be in linkagedisequilibrium with one or more other polymorphisms or polymorphicalleles.

“Diluent” means an ingredient in a composition that lackspharmacological activity, but is pharmaceutically necessary ordesirable. For example, the diluent in an injected composition may be aliquid, e.g. saline solution.

“Dose” means a specified quantity of a pharmaceutical agent provided ina single administration, or in a specified time period. In certainembodiments, a dose may be administered in one, two, or more boluses,tablets, or injections. For example, in certain embodiments wheresubcutaneous administration is desired, the desired dose requires avolume not easily accommodated by a single injection, therefore, two ormore injections may be used to achieve the desired dose. In certainembodiments, the pharmaceutical agent is administered by infusion overan extended period of time or continuously. Doses may be stated as theamount of pharmaceutical agent per hour, day, week, or month.

“Effective amount” means the amount of active pharmaceutical agentsufficient to effectuate a desired physiological outcome in anindividual in need of the agent. The effective amount may vary amongindividuals depending on the health and physical condition of theindividual to be treated, the taxonomic group of the individuals to betreated, the formulation of the composition, assessment of theindividual's medical condition, and other relevant factors.

“Fully complementary” or “100% complementary” means each nucleobase of afirst nucleic acid has a complementary nucleobase in a second nucleicacid. In certain embodiments, a first nucleic acid is an antisensecompound and a target nucleic acid is a second nucleic acid.

“Gapmer” means a chimeric antisense compound in which an internal regionhaving a plurality of nucleosides that support RNase H cleavage ispositioned between external regions having one or more nucleosides,wherein the nucleosides comprising the internal region are chemicallydistinct from the nucleoside or nucleosides comprising the externalregions. The internal region may be referred to as the “gap” and theexternal regions may be referred to as the “wings.”

“Gap-widened” means a chimeric antisense compound having a gap segmentof 12 or more contiguous 2′-deoxyribonucleosides positioned between andimmediately adjacent to 5′ and 3′ wing segments having from one to sixnucleosides.

“Gene product” refers to a biochemical material, such as RNA or protein,resulting from expression of a gene.

“Haplotype” means a set of alleles of closely linked loci on achromosome that are generally inherited together. For example, apolymorphic allele at a first site in a nucleic acid sequence on thechromosome may be found to be associated with another polymorphic alleleat a second site on the same chromosome, at a frequency other than wouldbe expected for a random associate (e.g. “linkage equilibrium”). Thesetwo polymorphic alleles may be described as being in “linkagedisequilibrium.” A haplotype may comprise two, three, four, or morealleles. The set of alleles in a haplotype along a given segment of achromosome are generally transmitted to progeny together unless therehas been a recombination event.

“High-affinity sugar modification” is a modified sugar moiety which whenit is included in a nucleoside and said nucleoside is incorporated intoan antisense oligonucleotide, the stability (as measured by Tm) of saidantisense oligonucleotide: RNA duplex is increased as compared to thestability of a DNA:RNA duplex.

“High-affinity sugar-modified nucleoside” is a nucleoside comprising amodified sugar moiety that when said nucleoside is incorporated into anantisense compound, the binding affinity (as measured by Tm) of saidantisense compound toward a complementary RNA molecule is increased. Incertain embodiments of the invention at least one of said sugar-modifiedhigh-affinity nucleosides confers a ΔTm of at least 1 to 4 degrees pernucleoside against a complementary RNA as determined in accordance withthe methodology described in Freier et al., Nucleic Acids Res., 1997,25, 4429-4443, which is incorporated by reference in its entirety. Inanother aspect, at least one of the high-affinity sugar modificationsconfers about 2 or more, 3 or more, or 4 or more degrees permodification. In the context of the present invention, examples ofsugar-modified high affinity nucleosides include, but are not limitedto, (i) certain 2′-modified nucleosides, including 2′-substituted and 4′to 2′ bicyclic nucleosides, and (ii) certain other non-ribofuranosylnucleosides which provide a per modification increase in bindingaffinity such as modified tetrahydropyran and tricycloDNA nucleosides.For other modifications that are sugar-modified high-affinitynucleosides see Freier et al., Nucleic Acids Res., 1997, 25, 4429-4443.

“Hybridization” means the annealing of complementary nucleic acidmolecules. In certain embodiments, complementary nucleic acid moleculesinclude an antisense compound and a target nucleic acid.

“Immediately adjacent” means there are no intervening elements betweenthe immediately adjacent elements.

“Individual” means a human or non-human animal selected for treatment ortherapy.

“Internucleoside linkage” refers to the chemical bond betweennucleosides.

“Linked nucleosides” means adjacent nucleosides which are bondedtogether.

“Mismatch” or “non-complementary nucleobase” refers to the case when anucleobase of a first nucleic acid is not capable of pairing with thecorresponding nucleobase of a second or target nucleic acid.

“Modified internucleoside linkage” refers to a substitution or anychange from a naturally occurring internucleoside bond (i.e. aphosphodiester internucleoside bond).

“Modified nucleobase” refers to any nucleobase other than adenine,cytosine, guanine, thymidine, or uracil. An “unmodified nucleobase”means the purine bases adenine (A) and guanine (G), and the pyrimidinebases thymine (T), cytosine (C), and uracil (U).

“Modified nucleotide” means a nucleotide having, independently, amodified sugar moiety, modified internucleoside linkage, or modifiednucleobase. A “modified nucleoside” means a nucleoside having,independently, a modified sugar moiety or modified nucleobase.

“Modified oligonucleotide” means an oligonucleotide comprising amodified internucleoside linkage, a modified sugar, or a modifiednucleobase.

“Modified sugar” refers to a substitution or change from a naturalsugar.

“Motif” means the pattern of chemically distinct regions in an antisensecompound.

“Naturally occurring internucleoside linkage” means a 3′ to 5′phosphodiester linkage.

“Natural sugar moiety” means a sugar found in DNA (2′-H) or RNA (2′-OH).

“Nuclease resistant modification” means a sugar modification or modifiedinternucleoside linkage which, when incorporated into anoligonucleotide, makes said oligonucleotide more stable to degradationunder cellular nucleases (e.g. exo- or endo-nucleases). Examples ofnuclease resistant modifications include, but are not limited to,phosphorothioate internucleoside linkages, bicyclic sugar modifications,2′-modified nucleotides, or neutral internucleoside linkages.

“Nucleic acid” refers to molecules composed of monomeric nucleotides. Anucleic acid includes ribonucleic acids (RNA), deoxyribonucleic acids(DNA), single-stranded nucleic acids, double-stranded nucleic acids,small interfering ribonucleic acids (siRNA), and microRNAs (miRNA).

“Nucleobase” means a heterocyclic moiety capable of pairing with a baseof another nucleic acid.

“Nucleobase sequence” means the order of contiguous nucleobasesindependent of any sugar, linkage, or nucleobase modification.

“Nucleoside” means a nucleobase linked to a sugar.

“Nucleoside mimetic” includes those structures used to replace the sugaror the sugar and the base and not necessarily the linkage at one or morepositions of an oligomeric compound such as for example nucleosidemimetics having morpholino, cyclohexenyl, cyclohexyl, tetrahydropyranyl,bicyclo or tricyclo sugar mimetics e.g. non furanose sugar units.Nucleotide mimetic includes those structures used to replace thenucleoside and the linkage at one or more positions of an oligomericcompound such as for example peptide nucleic acids or morpholinos(morpholinos linked by —N(H)—C(═O)—O— or other non-phosphodiesterlinkage). Sugar surrogate overlaps with the slightly broader termnucleoside mimetic but is intended to indicate replacement of the sugarunit (furanose ring) only. The tetrahydropyranyl rings provided hereinare illustrative of an example of a sugar surrogate wherein the furanosesugar group has been replaced with a tetrahydropyranyl ring system.

“Nucleotide” means a nucleoside having a phosphate group covalentlylinked to the sugar portion of the nucleoside.

“Oligomeric compound” or “oligomer” means a polymer of linked monomericsubunits which is capable of hybridizing to at least a region of anucleic acid molecule.

“Oligonucleotide” means a polymer of linked nucleosides each of whichcan be modified or unmodified, independent one from another.

“Parenteral administration” means administration through injection orinfusion. Parenteral administration includes subcutaneousadministration, intravenous administration, intramuscularadministration, intraarterial administration, intraperitonealadministration, or intracranial administration, e.g. intrathecal orintracerebroventricular administration.

“Peptide” means a molecule formed by linking at least two amino acids byamide bonds. Peptide refers to polypeptides and proteins.

“Pharmaceutical composition” means a mixture of substances suitable foradministering to an individual. For example, a pharmaceuticalcomposition may comprise one or more active pharmaceutical agents and asterile aqueous solution.

“Pharmaceutically acceptable salts” means physiologically andpharmaceutically acceptable salts of antisense compounds, i.e., saltsthat retain the desired biological activity of the parentoligonucleotide and do not impart undesired toxicological effectsthereto.

“Phosphorothioate linkage” means a linkage between nucleosides where thephosphodiester bond is modified by replacing one of the non-bridgingoxygen atoms with a sulfur atom. A phosphorothioate linkage (P═S) is amodified internucleoside linkage.

“Portion” means a defined number of contiguous (i.e. linked) nucleobasesof a nucleic acid. In certain embodiments, a portion is a defined numberof contiguous nucleobases of a target nucleic acid. In certainembodiments, a portion is a defined number of contiguous nucleobases ofan antisense compound.

“Prevent” refers to delaying or forestalling the onset or development ofa disease, disorder, or condition for a period of time from minutes toindefinitely. Prevent also means reducing risk of developing a disease,disorder, or condition.

“Prodrug” means a therapeutic agent that is prepared in an inactive formthat is converted to an active form within the body or cells thereof bythe action of endogenous enzymes or other chemicals or conditions.

“Selectively reducing expression of an allelic variant” means reducingexpression of one allele more than the other, differing allele among aset of alleles. For example, a mutant allele containing a singlenucleotide polymorphism (SNP) may be reduced more than a wild typeallele not containing the SNP.

“Side effects” means physiological responses attributable to a treatmentother than the desired effects. In certain embodiments, side effectsinclude injection site reactions, liver function test abnormalities,renal function abnormalities, liver toxicity, renal toxicity, centralnervous system abnormalities, myopathies, and malaise. For example,increased aminotransferase levels in serum may indicate liver toxicityor liver function abnormality. For example, increased bilirubin mayindicate liver toxicity or liver function abnormality.

“Single nucleotide polymorphism” or “SNP” means a single nucleotidevariation between the genomes of individuals of the same species. Insome cases, a SNP may be a single nucleotide deletion or insertion. Ingeneral, SNPs occur relatively frequently in genomes and thus contributeto genetic diversity. SNPs are thought to be mutationally more stablethan other polymorphisms, lending their use in association studies inwhich linkage disequilibrium between markers and an unknown variant isused to map disease-causing mutations. The location of a SNP isgenerally flanked by highly conserved sequences. An individual may behomozygous or heterozygous for an allele at each SNP site. Aheterozygous SNP allele can be a differentiating polymorphism. A SNP maybe targeted with an antisense oligonucleotide, meaning that the SNPanneals to (or aligns with) position 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, or 20 of the antisense oligonucleotide.The remainder of the antisense oligonucleotide bases must havesufficient complementarity to the SNP site to facilitate hybridization.

“Single nucleotide polymorphism position” or “SNP position” refers tothe nucleotide position of the SNP on a reference sequence.

“Single nucleotide polymorphism site” or “SNP site” refers to thenucleotides surrounding a SNP contained in a target nucleic acid towhich an antisense compound is targeted.

“Single-stranded oligonucleotide” means an oligonucleotide which is nothybridized to a complementary strand.

“Specifically hybridizable” refers to an antisense compound having asufficient degree of complementarity between an antisenseoligonucleotide and a target nucleic acid to induce a desired effect,while exhibiting minimal or no effects on non-target nucleic acids underconditions in which specific binding is desired, i.e. underphysiological conditions in the case of in vivo assays and therapeutictreatments.

“Targeting” or “targeted” means the process of design and selection ofan antisense compound that will specifically hybridize to a targetnucleic acid and induce a desired effect.

“Target nucleic acid,” “target RNA,” and “target RNA transcript” allrefer to a nucleic acid capable of being targeted by antisensecompounds.

“Target segment” means the sequence of nucleotides of a target nucleicacid to which an antisense compound is targeted. For example, for thepurposes of this patent application, the target segment may be withinthe SNP site. “5′ target site” refers to the 5′-most nucleotide of atarget segment. “3′ target site” refers to the 3′-most nucleotide of atarget segment.

“Therapeutically effective amount” means an amount of a pharmaceuticalagent that provides a therapeutic benefit to an individual.

“Treat” refers to administering a pharmaceutical composition to effectan alteration or improvement of a disease, disorder, or condition.

“Unmodified nucleotide” means a nucleotide composed of naturallyoccurring nucleobases, sugar moieties, and internucleoside linkages. Incertain embodiments, an unmodified nucleotide is an RNA nucleotide (i.e.β-D-ribonucleosides) or a DNA nucleotide (i.e. β-D-deoxyribonucleoside).

Certain Embodiments

Embodiments of the present invention provide methods, compounds, andcompositions for selectively inhibiting mRNA and protein expression ofan allelic variant of a gene or DNA sequence. In certain embodiments,the allelic variant contains a single nucleotide polymorphism (SNP). Incertain embodiments, the SNP is a differentiating polymorphism. Incertain embodiments, a SNP is associated with a mutant allele. Incertain embodiments, a SNP is in linkage disequilibrium with anotherpolymorphism that is associated with or is causative of disease. Incertain embodiments, a mutant allele is associated with disease. Incertain embodiments, mRNA and protein expression of a mutant allele isassociated with disease.

In certain embodiments, the expressed gene product of a mutant alleleresults in aggregation of the mutant proteins causing disease. Incertain embodiments, the expressed gene product of a mutant alleleresults in gain of function causing disease

In certain embodiments, selective reduction of mRNA and proteinexpression of a mutant allele is achieved by targeting a SNP located onthe mutant allele with an antisense compound. In certain embodiments,the antisense compound is an antisense oligonucleotide. In certainembodiments, the antisense compound is not a ribozyme, a double strandedsiRNA, or an shRNA. In certain embodiments, the antisenseoligonucleotide may have one or more modified sugar(s), nucleobase(s),or internucleoside linkage(s). In certain embodiments, the antisenseoligonucleotide is complementary to the SNP site. In certainembodiments, the antisense oligonucleotide is complementary to the SNPsite. In certain embodiments, the antisense oligonucleotide is at least65%, 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%,95%, 96%, 97%, 98%, or 99% complementary to the SNP site. In certainembodiments, the antisense oligonucleotide is 100% complementary to theSNP site. In certain embodiments, the SNP site is 12, 13, 14, 15, 16,17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleobases inlength. In certain embodiments, the SNP anneals to position 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 of theantisense oligonucleotide.

In certain embodiments, antisense compounds designed to selectivelyreduce an allelic variant of a gene containing a SNP are created basedon potency and selectivity of the antisense compound as well aspopulation genetics.

In certain embodiments, selective reduction of mRNA and proteinexpression of an allelic variant of a gene containing a SNP occurs in acell or tissue. In certain embodiments, the cell or tissue is in ananimal. In certain embodiments, the animal is a human.

In certain embodiments, described is a method of treating Huntington'sDisease, comprising selectively reducing expression of a mutanthuntingtin allele in an animal in need thereof, comprising administeringto the animal a compound, comprising a modified oligonucleotideconsisting of 15 to 20 linked nucleosides and fully complementary to themutant huntingtin allele, wherein position 5, 6, 7, 8, 9, 10, 11, 12,13, 14, or 15 of the modified oligonucleotide, as counted from the 5′terminus of the modified oligonucleotide, aligns with any of the SNPsites in the group consisting of rs6446723, rs3856973, rs2285086,rs363092, rs916171, rs6844859, rs7691627, rs4690073, rs2024115,rs11731237, rs362296, rs10015979, rs7659144, rs363096, rs362273,rs16843804, rs362271, rs362275, rs3121419, rs362272, rs3775061,rs34315806, rs363099, rs2298967, rs363088, rs363064, rs363102,rs2798235, rs363080, rs363072, rs363125, rs362303, rs362310, rs10488840,rs362325, rs35892913, rs1936032, rs2276881, rs363070, rs12502045,rs7685686, rs3733217, rs362331, rs2857936, rs12506200, rs762855,rs4690072, rs363081, rs363075, rs3025849, rs6855981, rs363144,rs3025838, rs2298969, rs362322, rs362307, rs362306, and rs1006798.

In certain embodiments, described is a compound comprising a modifiedoligonucleotide 15 to 20 linked nucleosides in length, targeted to ahuntingtin single nucleotide polymorphism, comprising a 12 nucleobaseportion of the nucleobase sequence of any one of SEQ ID NO: 6 to 285,wherein position 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 of themodified oligonucleotide, as counted from the 5′ terminus of themodified oligonucleotide, aligns with the huntingtin single nucleotidepolymorphism.

In certain embodiments, the modified oligonucleotide comprises awing-gap-wing motif of any one of the group consisting of 5-10-5, 2-9-6,3-9-3, 3-9-4, 3-9-5, 4-7-4, 4-9-3, 4-9-4, 4-9-5, 4-10-5, 4-11-4, 4-11-5,5-7-5, 5-8-6, 5-9-3, 5-9-5, 5-10-4, 5-10-5, 6-7-6, 6-8-5, and 6-9-2.

In certain embodiments, at least one internucleoside linkage is amodified internucleoside linkage. In certain embodiments, eachinternucleoside linkage is a phosphorothioate internucleoside linkage.

In certain embodiments, at least one nucleoside comprises a modifiednucleobase. In certain embodiments, the modified nucleobase is a5′-methylcytosine.

In certain embodiments, at least one nucleoside of at least one of thewing regions comprises a modified sugar or sugar surrogate. In certainembodiments, each of the nucleosides of each wing region comprises amodified sugar or sugar surrogate. In certain embodiments, the sugar orsugar surrogate is a 2′-O-methoxyethyl modified sugar. In certainembodiments, at least one of the wing regions comprises a 4′ to 2′bicyclic nucleoside and at least one of the remaining wing nucleosidesis a non-bicyclic 2′-modified nucleoside. In certain embodiments, thenon-bicyclic 2′-modified nucleoside is a 2′-O-methoxyethyl nucleoside.In certain embodiments, the 4′ to 2′ bicyclic nucleoside is4′-CH(CH3)-O-2′ bicyclic nucleoside.

In certain embodiments, described is a method of selectively reducingmutant huntingtin, comprising administering a compound comprising amodified oligonucleotide 15 to 20 linked nucleosides in length, targetedto a huntingtin single nucleotide polymorphism, comprising a 12nucleobase portion of the nucleobase sequence of any one of SEQ ID NO: 6to 285, wherein position 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 of themodified oligonucleotide, as counted from the 5′ terminus of themodified oligonucleotide, aligns with the huntingtin single nucleotidepolymorphism.

In certain embodiments, described is a use of a compound comprising amodified oligonucleotide 15 to 20 linked nucleosides in length, targetedto a huntingtin single nucleotide polymorphism, comprising a 12nucleobase portion of the nucleobase sequence of any one of SEQ ID NO: 6to 285, wherein position 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 of themodified oligonucleotide, as counted from the 5′ terminus of themodified oligonucleotide, aligns with the huntingtin single nucleotidepolymorphism for the treatment of Huntington's Disease.

Single Nucleotide Polymorphisms (SNPs)

Single-nucleotide polymorphisms (SNPs) are single base-pair alterationsin the DNA sequence that represent a major source of geneticheterogeneity (Gene. 1999, 234:177). SNP genotyping is an important toolwith which to investigate these genetic variants (Genome Res. 2000,10:895; Trends Biotechnol. 2000, 18:77). In certain embodiments,antisense compounds designed to selectively reduce an allelic variant ofa gene containing an SNP were selected based on potency, selectivity andpopulation genetics coverage.

Potency

In certain embodiments, antisense compounds designed to selectivelyreduce an allelic variant of a gene containing a SNP are created basedon potency of the antisense compound. Potency generally refers to howamenable the targeted sequence area is to antisense inhibition. Incertain embodiments, specific SNP sites may be particularly amenable toantisense inhibition. Certain such highly amenable SNP sites may betargeted by antisense compounds for selectively reducing an allelicvariant of a gene. Potency is demonstrated by the percent inhibition ofmutant mRNA achieved by the antisense oligonucleotides targeting a SNPcompared to the percent inhibition of mutant mRNA achieved by thebenchmark oligonucleotide.

Selectivity

In certain embodiments, antisense compounds designed to selectivelyreduce an allelic variant of a gene containing a SNP are created basedon selectivity of the antisense compound. Selectivity generally refersto antisense compounds comprising a particular sequence, motif, andchemical modification(s) that preferentially target the one or moredifferentiating polymorphisms (SNPs) in the RNA encoding a mutant HTTprotein compared to the RNA encoding a wild type HTT protein. In certainembodiments, specific sequences, motifs, and chemical modification(s)are particularly selective in reducing an allelic variant of a genecontaining a SNP. Certain such sequences, motifs, and chemicalmodification(s) are utilized to selectively reduce an allelic variant ofa gene. Selectivity is demonstrated by the ability of the antisenseoligonucleotide targeting a SNP to inhibit expression of the majorallele or mutant allele preferentially compared to the minor allele orwild type allele.

Population Genetics

In certain embodiments, antisense compounds designed to selectivelyreduce an allelic variant of a gene containing an SNP are created basedon the population genetics of a population afflicted with disease.Population genetics means the frequency at which the SNP appears in thedisease chromosome of patients afflicted with a particular disease. Incertain embodiments, the disease is Huntington disease. Where potencyand selectivity amongst antisense compounds is equal, SNP targets thathave higher population genetics coverage are favored over SNPs that havea weaker association with disease chromosomes.

Antisense Compounds

Oligomeric compounds may include, but are not limited to,oligonucleotides, oligonucleosides, oligonucleotide analogs,oligonucleotide mimetics, antisense compounds, antisenseoligonucleotides, and siRNAs. An oligomeric compound may be “antisense”to a target nucleic acid, meaning that is capable of undergoinghybridization to a target nucleic acid through hydrogen bonding.

In certain embodiments, an antisense compound is an antisenseoligonucleotide. In certain embodiments, the antisense compound is not aribozyme, a double stranded siRNA, or an shRNA.

In certain embodiments, an antisense compound has a nucleobase sequencethat, when written in the 5′ to 3′ direction, comprises the reversecomplement of the target segment of a target nucleic acid to which it istargeted. In certain such embodiments, an antisense oligonucleotide hasa nucleobase sequence that, when written in the 5′ to 3′ direction,comprises the reverse complement of the target segment of a targetnucleic acid to which it is targeted.

In certain embodiments, antisense compounds are 12 to 30 subunits inlength. In other words, such antisense compounds are from 12 to 30linked subunits. In other embodiments, the antisense compound is 8 to80, 12 to 50, 15 to 30, 18 to 24, 19 to 22, or 20 linked subunits. Incertain such embodiments, the antisense compounds are 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, or 80 linkedsubunits in length, or a range defined by any two of the above values.In some embodiments the antisense compound is an antisenseoligonucleotide, and the linked subunits are nucleosides.

In certain embodiments antisense oligonucleotides targeted to a nucleicacid may be shortened or truncated. For example, a single subunit may bedeleted from the 5′ end (5′ truncation), or alternatively from the 3′end (3′ truncation). A shortened or truncated antisense compoundtargeted to a nucleic acid may have two subunits deleted from the 5′end, or alternatively may have two subunits deleted from the 3′ end, ofthe antisense compound. Alternatively, the deleted nucleosides may bedispersed throughout the antisense compound, for example, in anantisense compound having one nucleoside deleted from the 5′ end and onenucleoside deleted from the 3′ end.

When a single additional subunit is present in a lengthened antisensecompound, the additional subunit may be located at the 5′ or 3′ end ofthe antisense compound. When two or more additional subunits arepresent, the added subunits may be adjacent to each other, for example,in an antisense compound having two subunits added to the 5′ end (5′addition), or alternatively to the 3′ end (3′ addition), of theantisense compound. Alternatively, the added subunits may be dispersedthroughout the antisense compound, for example, in an antisense compoundhaving one subunit added to the 5′ end and one subunit added to the 3′end.

It is possible to increase or decrease the length of an antisensecompound, such as an antisense oligonucleotide, and/or introducemismatch bases without eliminating activity. For example, in Woolf etal. (Proc. Natl. Acad. Sci. USA 89:7305-7309, 1992), a series ofantisense oligonucleotides 13-25 nucleobases in length were tested fortheir ability to induce cleavage of a target RNA in an oocyte injectionmodel. Antisense oligonucleotides 25 nucleobases in length with 8 or 11mismatch bases near the ends of the antisense oligonucleotides were ableto direct specific cleavage of the target mRNA, albeit to a lesserextent than the antisense oligonucleotides that contained no mismatches.Similarly, target specific cleavage was achieved using 13 nucleobaseantisense oligonucleotides, including those with 1 or 3 mismatches.

Gautschi et al (J. Natl. Cancer Inst. 93:463-471, March 2001)demonstrated the ability of an oligonucleotide having 100%complementarity to the bcl-2 mRNA and having 3 mismatches to the bcl-xLmRNA to reduce the expression of both bcl-2 and bcl-xL in vitro and invivo. Furthermore, this oligonucleotide demonstrated potent anti-tumoractivity in vivo.

Maher and Dolnick (Nuc. Acid. Res. 16:3341-3358, 1988) tested a seriesof tandem 14 nucleobase antisense oligonucleotides, and a 28 and 42nucleobase antisense oligonucleotides comprised of the sequence of twoor three of the tandem antisense oligonucleotides, respectively, fortheir ability to arrest translation of human DHFR in a rabbitreticulocyte assay. Each of the three 14 nucleobase antisenseoligonucleotides alone was able to inhibit translation, albeit at a moremodest level than the 28 or 42 nucleobase antisense oligonucleotides.

However, selective reduction of expression of an allelic variant isoptimized when the SNP contained in the target nucleic anneals to acomplementary base in the antisense compound and not a mismatched base.Moreover, selectivity in general is increased when there are fewermismatches between the SNP site and the antisense compound. However, acertain number of mismatches may be tolerated.

Antisense Compound Motifs

In certain embodiments, antisense compounds targeted to a nucleic acidhave chemically modified subunits arranged in patterns, or motifs, toconfer to the antisense compounds properties such as enhanced theinhibitory activity, increased binding affinity for a target nucleicacid, or resistance to degradation by in vivo nucleases.

Chimeric antisense compounds typically contain at least one regionmodified so as to confer increased resistance to nuclease degradation,increased cellular uptake, increased binding affinity for the targetnucleic acid, and/or increased inhibitory activity. A second region of achimeric antisense compound may optionally serve as a substrate for thecellular endonuclease RNase H, which cleaves the RNA strand of anRNA:DNA duplex.

Antisense compounds having a gapmer motif are considered chimericantisense compounds. In a gapmer an internal region having a pluralityof nucleotides that supports RNaseH cleavage is positioned betweenexternal regions having a plurality of nucleotides that are chemicallydistinct from the nucleosides of the internal region. In the case of anantisense oligonucleotide having a gapmer motif, the gap segmentgenerally serves as the substrate for endonuclease cleavage, while thewing segments comprise modified nucleosides. In the case of an antisenseoligonucleotide for selectively reducing expression of an allelicvariant of a gene containing a SNP, the SNP anneals to a nucleobasewithin the gap segment.

In certain embodiments, the SNP anneals or is complementary to anucleobase at position 5, 6, 7, 8, 9, 10, 11, 12, 13, or 14 of theantisense oligonucleotide, wherein position refers to the orientation ofa nucleobase within the antisense oligonucleotide counting from the 5′terminus of the antisense oligonucleotide. For example, the 5′ mostnucleobase within the antisense oligonucleotide is in the first positionof the antisense oligonucleotide. In certain embodiments, the SNPanneals or is complementary to a nucleobase at position 6, 7, 8, 9, or10 of the antisense oligonucleotide (counting from the 5′ terminus). Incertain embodiments, the SNP anneals or is complementary to a nucleobaseat position 9 or 10 of the antisense oligonucleotide (counting from the5′ terminus).

In certain embodiments, the SNP anneals to a nucleobase at position 1,2, 3, 4, 5, 6, 7, 8, 9, or 10 of the gap segment, wherein positionrefers to the orientation of a nucleobase within the gap segmentcounting from the 5′ terminus of the gap segment. For example, the 5′most nucleobase within the gap segment is in the first position of thegap segment. In certain embodiments, the SNP anneals to a nucleobase atposition 4, 5, 6, or 7 counting from the 5′ terminus of the gap segment.In certain embodiments, the SNP anneals to a nucleobase at position 4 or5 beginning from the 5′ terminus of the gap segment.

In certain embodiments, the regions of a gapmer are differentiated bythe types of sugar moieties comprising each distinct region. The typesof sugar moieties that are used to differentiate the regions of a gapmermay in some embodiments include β-D-ribonucleosides,β-D-deoxyribonucleosides, 2′-modified nucleosides (such 2′-modifiednucleosides may include 2′-MOE, and 2′-O—CH₃, among others), andbicyclic sugar modified nucleosides (such bicyclic sugar modifiednucleosides may include those having a 4′-(CH₂)n-O-2′ bridge, where n=1or n=2). The bicyclic moiety may be a cEt having the formula4′-CH(CH₃)—O-2.′

The wing-gap-wing motif is frequently described as “X-Y-Z”, where “X”represents the length of the 5′ wing region, “Y” represents the lengthof the gap region, and “Z” represents the length of the 3′ wing region.As used herein, a gapmer described as “X-Y-Z” has a configuration suchthat the gap segment is positioned immediately adjacent to each of the5′ wing segment and the 3′ wing segment. Thus, no interveningnucleotides exist between the 5′ wing segment and gap segment, or thegap segment and the 3′ wing segment. Any of the antisense compoundsdescribed herein can have a gapmer motif. In some embodiments, X and Zare the same, in other embodiments they are different. In certainembodiments, Y is between 8 and 15 nucleotides. In certain embodiments,Y is comprised of deoxynucleotides. X, Y or Z can be any of 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30 ormore nucleotides. Thus, gapmers of the present invention include, butare not limited to, for example 1-10-1, 1-18-1, 2-8-2, 2-9-6, 2-10-2,2-13-5, 2-16-2, 3-9-3, 3-9-5, 3-10-3, 3-14-3, 4-8-4, 4-9-5, 4-10-5,4-11-4, 4-12-3, 4-12-4, 5-8-5, 5-9-5, 5-10-4, 5-10-5, or 6-8-6.

In certain embodiments, the antisense compound has a “wingmer” motif,having a wing-gap or gap-wing configuration, i.e. an X-Y or Y-Zconfiguration as described above for the gapmer configuration. Thus,wingmer configurations of the present invention include, but are notlimited to, for example 5-10, 8-4, 4-12, 12-4, 3-14, 16-2, 18-1, 10-3,2-10, 1-10, 8-2, 2-13, 5-13, 5-8, or 6-8.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 2-9-6 gapmer motif or a 6-9-2 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 3-9-3 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 3-9-5 gapmer motif or 5-9-3 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 4-9-5 gapmer motif or 5-9-4 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 4-10-5 gapmer motif or 5-10-4 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 4-11-4 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 5-9-5 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 5-8-6 gapmer motif or a 6-8-5 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 6-7-6 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 6-8-5 gapmer motif or a 5-8-6 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 3-9-4 gapmer motif or a 4-9-3 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 5-7-5 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 4-7-4 gapmer motif.

In certain embodiments, antisense compounds targeted to a nucleic acidpossess a 5-10-5 gapmer motif.

In certain embodiments, an antisense compound targeted to a nucleic acidhas a gap-widened motif.

Certain Mixed Wings

In certain embodiments, the invention provides gapmer compounds whereinat least one nucleoside of one wing is differently modified compared toat least one other nucleoside of the same wing. Such antisense compoundsare referred to as mixed wing antisense compounds (see WO 2008/049085).In certain embodiments, the modifications (or no modification) of one ormore nucleosides of the 3′ wing are different from those of one or moreother nucleosides of the 3′ wing. Such antisense compounds may bereferred to as 3′ mixed wing gapmers. In certain embodiments, themodifications (or no modification) of one or more nucleosides of the 5′wing are different from those of one or more other nucleosides of the 5′wing. Such antisense compounds may be referred to as 5′ mixed winggapmers. In certain embodiments, the modifications (or no modification)of one or more nucleosides of the 3′ wing are different from those ofone or more other nucleosides of the 3′ wing and the modifications (orno modification) of one or more nucleosides of the 5′ wing are differentfrom those of one or more other nucleosides of the 5′ wing. Suchantisense compounds may be referred to as 3′, 5′ mixed wing gapmers. Insuch embodiment, the modifications and combination of modifications atthe 3′ wing and at the 5′ wing may be the same or they may be different.

In certain embodiments, mixed wing compounds have desirable properties.Certain nucleoside modifications confer on the antisense compound adesirable property, for example increased affinity for a target ornuclease resistance, but also confer an undesirable property, forexample increased toxicity. Incorporation of certain other nucleosidemodifications results in antisense compounds with different profiles ofproperties. In certain embodiments, one may combine modifications in oneor both wings to optimize desirable characteristics and/or minimizeundesirable characteristics. In certain embodiments, the wings of amixed wing antisense compound comprise one or more nucleoside comprisinga first modification that increases affinity of the antisense compoundfor a target nucleic acid compared to an antisense compound comprisingunmodified nucleosides; and one or more nucleoside comprising a secondmodification that results in reduced toxicity compared to an antisensecompound with wings comprising nucleosides that all comprise the firstmodification.

In certain embodiments, an antisense compound comprises at least onewing comprising at least one MOE substituted nucleoside and at least onehigh affinity modification. In certain such embodiments, the at leastone MOE substituted nucleoside and the at least one high affinity are inthe 3′ wing. In certain such embodiments, the at least one MOEsubstituted nucleoside and the at least one high affinity are in the 5′wing.

In certain embodiments, an antisense compound comprises 1, 2 or 3 highaffinity modifications in the 5′ and/or 3′ wings.

Target Nucleic Acids, Target Regions and Nucleotide Sequences

In certain embodiments, an allelic variant of huntingtin is selectivelyreduced. Nucleotide sequences that encode huntingtin include, withoutlimitation, the following: GENBANK Accession No. NT_(—)006081.18,truncated from nucleotides 1566000 to 1768000 (replaced by GENBANKAccession No. NT_(—)006051), incorporated herein as SEQ ID NO: 1, andNM_(—)002111.6, incorporated herein as SEQ ID NO: 2.

It is understood that the sequence set forth in each SEQ ID NO in theExamples contained herein is independent of any modification to a sugarmoiety, an internucleoside linkage, or a nucleobase. As such, antisensecompounds defined by a SEQ ID NO may comprise, independently, one ormore modifications to a sugar moiety, an internucleoside linkage, or anucleobase. Antisense compounds described by Isis Number (Isis No)indicate a combination of nucleobase sequence and motif.

In certain embodiments, a target region is a structurally defined regionof the target nucleic acid. For example, a target region may encompass a3′ UTR, a 5′ UTR, an exon, an intron, an exon/intron junction, a codingregion, a translation initiation region, translation termination region,or other defined nucleic acid region. The structurally defined regionsfor huntingtin can be obtained by accession number from sequencedatabases such as NCBI and such information is incorporated herein byreference. In certain embodiments, a target region may encompass thesequence from a 5′ target site of one target segment within the targetregion to a 3′ target site of another target segment within the sametarget region.

Targeting includes determination of at least one target segment to whichan antisense compound hybridizes, such that a desired effect occurs. Incertain embodiments, the desired effect is a reduction in mRNA targetnucleic acid levels of a particular allelic variant. In certainembodiments, the desired effect is reduction of levels of the proteinencoded by the target nucleic acid or a phenotypic change associatedwith a particular alleleic variant.

A target region may contain one or more target segments. Multiple targetsegments within a target region may be overlapping. Alternatively, theymay be non-overlapping. In certain embodiments, target segments within atarget region are separated by no more than about 300 nucleotides. Incertain embodiments, target segments within a target region areseparated by a number of nucleotides that is, is about, is no more than,is no more than about, 250, 200, 150, 100, 90, 80, 70, 60, 50, 40, 30,20, or 10 nucleotides on the target nucleic acid, or is a range definedby any two of the preceeding values. In certain embodiments, targetsegments within a target region are separated by no more than, or nomore than about, 5 nucleotides on the target nucleic acid. In certainembodiments, target segments are contiguous. Contemplated are targetregions defined by a range having a starting nucleic acid that is any ofthe 5′ target sites or 3′ target sites listed herein.

Suitable target segments may be found within a 5′ UTR, a coding region,a 3′ UTR, an intron, an exon, or an exon/intron junction. Targetsegments containing a start codon or a stop codon are also suitabletarget segments. A suitable target segment may specifically exclude acertain structurally defined region such as the start codon or stopcodon.

The determination of suitable target segments may include a comparisonof the sequence of a target nucleic acid to other sequences throughoutthe genome. For example, the BLAST algorithm may be used to identifyregions of similarity amongst different nucleic acids. This comparisoncan prevent the selection of antisense compound sequences that mayhybridize in a non-specific manner to sequences other than a selectedtarget nucleic acid (i.e., non-target or off-target sequences).

Cell Lines

In certain embodiments, the GM04281, GM02171, and GM02173B cell linesare used in experiments described herein below. The GM04281 cell linehas a wild-type HTT allele that contains 17 repeats and a mutant HTTallele that contains 69 repeats. The cell line was derived from apatient both of whose parents were also affected by the disease. TheGM02171 cell line was chosen as a counter screen control to the GM04281.This cell line was derived from the daughter of parents, only one ofwhom had the disease. The daughter had not developed HD but wasconsidered to be at risk. The GM02173B cell line was alsopatient-derived and was used as a haplotype test control.

Table 1 provides SNPs found in the GM04281, GM02171, and GM02173B celllines. Also provided are the allelic variants found at each SNPposition, the genotype for each of the cell lines, and the percentage ofHD patients having a particular allelic variant. For example, the twoallelic variants for SNP rs6446723 are T and C. The GM02171 cell line ishomozygous CC, the GM02173 cell line is heterozygous TC, and the GM04281cell line is homozygous TT. Fifty percent of HD patients have a T at SNPposition rs6446723.

TABLE 1 Allelic Variations for SNPs Associated with HD SNP VariationGM02171 GM02173 GM04281 TargetPOP allele rs6446723 T/C CC TC TT 0.50 Trs3856973 A/G AA AG GG 0.50 G rs2285086 A/G GG AG AA 0.50 A rs363092 A/CAA AC CC 0.49 C rs916171 C/G GG GC CC 0.49 C rs6844859 T/C CC TC TT 0.49T rs7691627 A/G AA AG GG 0.49 G rs4690073 A/G AA AG GG 0.49 G rs2024115A/G GG AG AA 0.48 A rs11731237 T/C CC TC TT 0.43 T rs362296 A/C AC AC AC0.42 C rs10015979 A/G AA AG GG 0.42 G rs7659144 C/G CG CG CC 0.41 Crs363096 T/C CC TC TT 0.40 T rs362273 A/G AG AG AA 0.39 A rs16843804 T/CTC TC CC 0.38 C rs362271 A/G AG AG GG 0.38 G rs362275 T/C TC TC CC 0.38C rs3121419 T/C TC TC CC 0.38 C rs362272 A/G — AG GG 0.38 G rs3775061A/G AG AG AA 0.38 A rs34315806 T/C TC TC CC 0.38 C rs363099 T/C TC TC CC0.38 C rs2298967 T/C TC TC TT 0.38 T rs363088 A/T TA TA AA 0.38 Ars363064 T/C TC TC CC 0.35 C rs363102 A/G AA AA AA 0.23 G rs2798235 A/GGG GG GG 0.21 A rs363080 T/C CC CC CC 0.21 T rs363072 A/T TA AA AA 0.13A rs363125 A/C AC CC CC 0.12 C rs362303 T/C TC CC CC 0.12 C rs362310 T/CTC CC CC 0.12 C rs10488840 A/G AG GG GG 0.12 G rs362325 T/C TC TT TT0.11 T rs35892913 A/G GG GG GG 0.10 A rs363102 A/G AA AA AA 0.09 Ars363096 T/C CC TC TT 0.09 C rs11731237 T/C CC TC TT 0.09 C rs10015979A/G AA AG GG 0.08 A rs363080 T/C CC CC CC 0.07 C rs2798235 A/G GG GG GG0.07 G rs1936032 C/G CC CC CC 0.06 C rs2276881 A/G GG GG GG 0.06 Grs363070 A/G AA AA AA 0.06 A rs35892913 A/G GG GG GG 0.04 G rs12502045T/C CC CC CC 0.04 C rs6446723 T/C CC TC TT 0.04 C rs7685686 A/G GG AG AA0.04 G rs3733217 T/C CC CC CC 0.03 C rs6844859 T/C CC TC TT 0.03 Crs362331 T/C CC TC TT 0.03 C

Hybridization

In some embodiments, hybridization occurs between an antisense compounddisclosed herein and a SNP site. The most common mechanism ofhybridization involves hydrogen bonding (e.g., Watson-Crick, Hoogsteenor reversed Hoogsteen hydrogen bonding) between complementarynucleobases of the nucleic acid molecules.

Hybridization can occur under varying conditions. Stringent conditionsare sequence-dependent and are determined by the nature and compositionof the nucleic acid molecules to be hybridized.

In certain embodiments, the antisense compounds provided herein arespecifically hybridizable with the nucleic acid of a particular allelicvariant.

Complementarity

An antisense compound and a target nucleic acid are complementary toeach other when a sufficient number of nucleobases of the antisensecompound can hydrogen bond with the corresponding nucleobases of thetarget nucleic acid, such that a desired effect will occur (e.g.,selective reduction of a gene product of an allelic variant).

Non-complementary nucleobases between an antisense compound and a targetnucleic acid may be tolerated provided that the antisense compoundremains able to specifically hybridize to a target nucleic acid.Moreover, an antisense compound may hybridize over one or more segmentsof a target nucleic acid such that intervening or adjacent segments arenot involved in the hybridization event (e.g., a loop structure,mismatch or hairpin structure).

In certain embodiments, the antisense compounds provided herein, or aspecified portion thereof, are, or are at least, 70%, 80%, 85%, 86%,87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%complementary to a target nucleic acid, a target region, target segment,SNP site, or specified portion thereof. Percent complementarity of anantisense compound with a target nucleic acid can be determined usingroutine methods.

For example, an antisense compound in which 18 of 20 nucleobases of theantisense compound are complementary to a target region, and wouldtherefore specifically hybridize, would represent 90 percentcomplementarity. In this example, the remaining noncomplementarynucleobases may be clustered or interspersed with complementarynucleobases and need not be contiguous to each other or to complementarynucleobases. As such, an antisense compound which is 18 nucleobases inlength having 4 (four) noncomplementary nucleobases which are flanked bytwo regions of complete complementarity with the target nucleic acidwould have 77.8% overall complementarity with the target nucleic acidand would thus fall within the scope of the present invention. Percentcomplementarity of an antisense compound with a region of a targetnucleic acid can be determined routinely using BLAST programs (basiclocal alignment search tools) and PowerBLAST programs known in the art(Altschul et al., J. Mol. Biol., 1990, 215, 403 410; Zhang and Madden,Genome Res., 1997, 7, 649 656). Percent homology, sequence identity orcomplementarity, can be determined by, for example, the Gap program(Wisconsin Sequence Analysis Package, Version 8 for Unix, GeneticsComputer Group, University Research Park, Madison Wis.), using defaultsettings, which uses the algorithm of Smith and Waterman (Adv. Appl.Math., 1981, 2, 482 489).

In certain embodiments, the antisense compounds provided herein, orspecified portions thereof, are fully complementary (i.e. 100%complementary) to a target nucleic acid, a SNP site, target region,target segment, or specified portion thereof. As used herein, “fullycomplementary” means each nucleobase of an antisense compound is capableof precise base pairing with the corresponding nucleobases of a targetnucleic acid. For example, a 20 nucleobase antisense compound is fullycomplementary to a target sequence that is 400 nucleobases long, so longas there is a corresponding 20 nucleobase portion of the target nucleicacid that is fully complementary to the antisense compound. Fullycomplementary can also be used in reference to a specified portion ofthe first and/or the second nucleic acid. For example, a 20 nucleobaseportion of a 30 nucleobase antisense compound can be “fullycomplementary” to a target sequence that is 400 nucleobases long. The 20nucleobase portion of the 30 nucleobase oligonucleotide is fullycomplementary to the target sequence if the target sequence has acorresponding 20 nucleobase portion wherein each nucleobase iscomplementary to the 20 nucleobase portion of the antisense compound. Atthe same time, the entire 30 nucleobase antisense compound may or maynot be fully complementary to the target sequence, depending on whetherthe remaining 10 nucleobases of the antisense compound are alsocomplementary to the target sequence.

The location of a non-complementary nucleobase may be at the 5′ end or3′ end of the antisense compound. Alternatively, the non-complementarynucleobase or nucleobases may be at an internal position of theantisense compound. When two or more non-complementary nucleobases arepresent, they may be contiguous (i.e. linked) or non-contiguous. In oneembodiment, a non-complementary nucleobase is located in the wingsegment of a gapmer antisense oligonucleotide.

In certain embodiments, antisense compounds that are, or are up to 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or30 nucleobases in length comprise no more than 6, no more than 5, nomore than 4, no more than 3, no more than 2, or no more than 1non-complementary nucleobase(s) relative to a target nucleic acid, SNPsite, or specified portion thereof.

In certain embodiments, antisense oligonucleotides that are, or are upto 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, or 30 nucleobases in length comprise no more than 6, no more than 5,no more than 4, no more than 3, no more than 2, or no more than 1non-complementary nucleobase(s) relative to a target nucleic acid, SNPsite, or specified portion thereof.

The antisense compounds provided herein also include those which arecomplementary to a portion of a target nucleic acid. As used herein,“portion” refers to a defined number of contiguous (i.e. linked)nucleobases within a region or segment of a target nucleic acid. A“portion” can also refer to a defined number of contiguous nucleobasesof an antisense compound. In certain embodiments, the antisensecompounds, are complementary to at least an 8 nucleobase portion of atarget segment. In certain embodiments, the antisense compounds arecomplementary to at least a 12 nucleobase portion of a target segment.In certain embodiments, the antisense compounds are complementary to atleast a 15 nucleobase portion of a target segment. Also contemplated areantisense compounds that are complementary to at least a 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, or more nucleobase portion of a targetsegment, or a range defined by any two of these values.

Identity

The antisense compounds provided herein may also have a defined percentidentity to a particular nucleotide sequence, SEQ ID NO, or compoundrepresented by a specific Isis number, or portion thereof. As usedherein, an antisense compound is identical to the sequence disclosedherein if it has the same nucleobase pairing ability. For example, a RNAwhich contains uracil in place of thymidine in a disclosed DNA sequencewould be considered identical to the DNA sequence since both uracil andthymidine pair with adenine. Shortened and lengthened versions of theantisense compounds described herein as well as compounds havingnon-identical bases relative to the antisense compounds provided hereinalso are contemplated. The non-identical bases may be adjacent to eachother or dispersed throughout the antisense compound. Percent identityof an antisense compound is calculated according to the number of basesthat have identical base pairing relative to the sequence to which it isbeing compared.

In certain embodiments, the antisense compounds, or portions thereof,are at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100%identical to one or more of the antisense compounds or SEQ ID NOs, or aportion thereof, disclosed herein.

In certain embodiments, a portion of the antisense compound is comparedto an equal length portion of the target nucleic acid. In certainembodiments, an 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21,22, 23, 24, or 25 nucleobase portion is compared to an equal lengthportion of the target nucleic acid.

In certain embodiments, a portion of the antisense oligonucleotide iscompared to an equal length portion of the target nucleic acid. Incertain embodiments, an 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,20, 21, 22, 23, 24, or 25 nucleobase portion is compared to an equallength portion of the target nucleic acid.

Modifications

A nucleoside is a base-sugar combination. The nucleobase (also known asbase) portion of the nucleoside is normally a heterocyclic base moiety.Nucleotides are nucleosides that further include a phosphate groupcovalently linked to the sugar portion of the nucleoside. For thosenucleosides that include a pentofuranosyl sugar, the phosphate group canbe linked to the 2′, 3′ or 5′ hydroxyl moiety of the sugar.Oligonucleotides are formed through the covalent linkage of adjacentnucleosides to one another, to form a linear polymeric oligonucleotide.Within the oligonucleotide structure, the phosphate groups are commonlyreferred to as forming the internucleoside linkages of theoligonucleotide.

Modifications to antisense compounds encompass substitutions or changesto internucleoside linkages, sugar moieties, or nucleobases. Modifiedantisense compounds are often preferred over native forms because ofdesirable properties such as, for example, enhanced cellular uptake,enhanced affinity for nucleic acid target, increased stability in thepresence of nucleases, or increased inhibitory activity.

Chemically modified nucleosides may also be employed to increase thebinding affinity of a shortened or truncated antisense oligonucleotidefor its target nucleic acid. Consequently, comparable results can oftenbe obtained with shorter antisense compounds that have such chemicallymodified nucleosides.

Chemically modified nucleosides may also be employed to increaseselectivity in reducing expression the gene product of an allelicvariant.

Modified Internucleoside Linkages

The naturally occurring internucleoside linkage of RNA and DNA is a 3′to 5′ phosphodiester linkage. Antisense compounds having one or moremodified, i.e. non-naturally occurring, internucleoside linkages areoften selected over antisense compounds having naturally occurringinternucleoside linkages because of desirable properties such as, forexample, enhanced cellular uptake, enhanced affinity for target nucleicacids, and increased stability in the presence of nucleases.

Oligonucleotides having modified internucleoside linkages includeinternucleoside linkages that retain a phosphorus atom as well asinternucleoside linkages that do not have a phosphorus atom.Representative phosphorus containing internucleoside linkages include,but are not limited to, phosphodiesters, phosphotriesters,methylphosphonates, phosphoramidate, and phosphorothioate. Methods ofpreparation of phosphorous-containing and non-phosphorous-containinglinkages are well known.

In certain embodiments, antisense compounds comprise one or moremodified internucleoside linkages. In certain embodiments, the modifiedinternucleoside linkages are phosphorothioate linkages. In certainembodiments, each internucleoside linkage of an antisense compound is aphosphorothioate internucleoside linkage.

Modified Sugar Moieties

Antisense compounds of the invention can optionally contain one or morenucleosides wherein the sugar group has been modified. Such sugarmodified nucleosides may impart enhanced nuclease stability, increasedbinding affinity, increased selectivity for an allelic variant, or someother beneficial biological property to the antisense compounds. Incertain embodiments, nucleosides comprise a chemically modifiedribofuranose ring moieties. Examples of chemically modified ribofuranoserings include without limitation, addition of substitutent groups(including 5′ and 2′ substituent groups, bridging of non-geminal ringatoms to form bicyclic nucleic acids (BNA), replacement of the ribosylring oxygen atom with S, N(R), or C(R1)(R)2 (R═H, C1-C12 alkyl or aprotecting group) and combinations thereof. Examples of chemicallymodified sugars include 2′-F-5′-methyl substituted nucleoside (see PCTInternational Application WO 2008/101157 Published on Aug. 21, 2008 forother disclosed 5′,2′-bis substituted nucleosides) or replacement of theribosyl ring oxygen atom with S with further substitution at the2′-position (see published U.S. Patent Application US2005-0130923,published on Jun. 16, 2005) or alternatively 5′-substitution of a BNA(see PCT International Application WO 2007/134181 Published on Nov. 22,2007 wherein LNA is substituted with for example a 5′-methyl or a5′-vinyl group).

Examples of nucleosides having modified sugar moieties include withoutlimitation nucleosides comprising 5′-vinyl, 5′-methyl (R or S), 4′-S,2′-F, 2′-OCH3 and 2′-O(CH2)2OCH3 substituent groups. The substituent atthe 2′ position can also be selected from allyl, amino, azido, thio,O-allyl, O—C1-C10 alkyl, OCF3, O(CH2)2SCH3, O(CH2)2-O—N(Rm)(Rn), andO—CH2-C(═O)—N(Rm)(Rn), where each Rm and Rn is, independently, H orsubstituted or unsubstituted C1-C10 alkyl.

As used herein, “bicyclic nucleosides” refer to modified nucleosidescomprising a bicyclic sugar moiety. Examples of bicyclic nucleosidesinclude without limitation nucleosides comprising a bridge between the4′ and the 2′ ribosyl ring atoms. In certain embodiments, antisensecompounds provided herein include one or more bicyclic nucleosideswherein the bridge comprises a 4′ to 2′ bicyclic nucleoside. Examples ofsuch 4′ to 2′ bicyclic nucleosides, include but are not limited to oneof the formulae: 4′-(CH₂)—O-2′ (LNA); 4′-(CH₂)—S-2′; 4′-(CH₂)₂—O-2′(ENA); 4′-CH(CH₃)—O-2′ and 4′-CH(CH₂OCH₃)—O-2′ (and analogs thereof seeU.S. Pat. No. 7,399,845, issued on Jul. 15, 2008); 4′-C(CH₃)(CH₃)—O-2′(and analogs thereof see published International ApplicationWO/2009/006478, published Jan. 8, 2009); 4′-CH₂—N(OCH₃)-2′ (and analogsthereof see published International Application WO/2008/150729,published Dec. 11, 2008); 4′-CH₂—O—N(CH₃)-2′ (see published U.S. PatentApplication US2004-0171570, published Sep. 2, 2004); 4′-CH₂—N(R)—O-2′,wherein R is H, C₁-C₁₂ alkyl, or a protecting group (see U.S. Pat. No.7,427,672, issued on Sep. 23, 2008); 4′-CH₂—C(H)(CH₃)-2′ (seeChattopadhyaya, et al., J. Org. Chem., 2009, 74, 118-134); and4′-CH₂—C(═CH₂)-2′ (and analogs thereof see published InternationalApplication WO 2008/154401, published on Dec. 8, 2008). See, forexample: Singh et al., Chem. Commun., 1998, 4, 455-456; Koshkin et al.,Tetrahedron, 1998, 54, 3607-3630; Wahlestedt et al., Proc. Natl. Acad.Sci. U.S.A., 2000, 97, 5633-5638; Kumar et al., Bioorg. Med. Chem.Lett., 1998, 8, 2219-2222; Singh et al., J. Org. Chem., 1998, 63,10035-10039; Srivastava et al., J. Am. Chem. Soc., 129(26) 8362-8379(Jul. 4, 2007); U.S. Pat. Nos. 7,053,207; 6,268,490; 6,770,748;6,794,499; 7,034,133; and 6,525,191; Elayadi et al., Curr. OpinionInvens. Drugs, 2001, 2, 558-561; Braasch et al., Chem. Biol., 2001, 8,1-7; and Orum et al., Curr. Opinion Mol. Ther., 2001, 3, 239-243; andU.S. Pat. No. 6,670,461; International applications WO 2004/106356; WO94/14226; WO 2005/021570; U.S. Patent Publication Nos. US2004-0171570;US2007-0287831; US2008-0039618; U.S. Pat. No. 7,399,845; U.S. patentSer. Nos. 12/129,154; 60/989,574; 61/026,995; 61/026,998; 61/056,564;61/086,231; 61/097,787; 61/099,844; PCT International Applications Nos.PCT/US2008/064591; PCT/US2008/066154; PCT/US2008/068922; and PublishedPCT International Applications WO 2007/134181. Each of the foregoingbicyclic nucleosides can be prepared having one or more stereochemicalsugar configurations including for example α-L-ribofuranose andβ-D-ribofuranose (see PCT international application PCT/DK98/00393,published on Mar. 25, 1999 as WO 99/14226).

In certain embodiments, bicyclic sugar moieties of BNA nucleosidesinclude, but are not limited to, compounds having at least one bridgebetween the 4′ and the 2′ position of the pentofuranosyl sugar moietywherein such bridges independently comprises 1 or from 2 to 4 linkedgroups independently selected from —[C(R_(a))(R_(b))]_(n)—,—C(R_(a))═C(R_(b))—, —C(R_(a))═N—, —C(═NR_(a))—, —C(═O)—, —C(═S)—, —O—,—Si(R_(a))₂—, —S(═O)_(x)—, and —N(R_(a))—;

wherein:

x is 0, 1, or 2;

n is 1, 2, 3, or 4;

each R_(a) and R_(b) is, independently, H, a protecting group, hydroxyl,C₁-C₁₂ alkyl, substituted C₁-C₁₂ alkyl, C₂-C₁₂ alkenyl, substitutedC₂-C₁₂ alkenyl, C₂-C₁₂ alkynyl, substituted C₂-C₁₂ alkynyl, C₅-C₂₀ aryl,substituted C₅-C₂₀ aryl, heterocycle radical, substituted heterocycleradical, heteroaryl, substituted heteroaryl, C₅-C₇ alicyclic radical,substituted C₅-C₇ alicyclic radical, halogen, OJ₁, NJ₁J₂, SJ₁, N₃,COOJ₁, acyl (C(═O)—H), substituted acyl, CN, sulfonyl (S(═O)₂-J₁), orsulfoxyl (S(═O)-J₁); and

each J₁ and J₂ is, independently, H, C₁-C₁₂ alkyl, substituted C₁-C₁₂alkyl, C₂-C₁₂ alkenyl, substituted C₂-C₁₂ alkenyl, C₂-C₁₂ alkynyl,substituted C₂-C₁₂ alkynyl, C₅-C₂₀ aryl, substituted C₅-C₂₀ aryl, acyl(C(═O)—H), substituted acyl, a heterocycle radical, a substitutedheterocycle radical, C₁-C₁₂ aminoalkyl, substituted C₁-C₁₂ aminoalkyl ora protecting group.

In certain embodiments, the bridge of a bicyclic sugar moiety is,—[C(R_(a))(R_(b))]_(n)—, —[C(R_(a))(R_(b))]_(n)—O—,—C(R_(a)R_(b))—N(R)—O— or —C(R_(a)R_(b))—O—N(R)—. In certainembodiments, the bridge is 4′-CH₂-2′, 4′-(CH₂)₂-2′, 4′-(CH₂)₃-2′,4′-CH₂—O-2′, 4′-(CH₂)₂—O-2′, 4′-CH₂—O—N(R)-2′ and 4′-CH₂—N(R)—O-2′-wherein each R is, independently, H, a protecting group or C₁-C₁₂ alkyl.

In certain embodiments, bicyclic nucleosides are further defined byisomeric configuration. For example, a nucleoside comprising a 4′-2′methylene-oxy bridge, may be in the α-L configuration or in the β-Dconfiguration. Previously, α-L-methyleneoxy (4′-CH₂—O-2′) BNA's havebeen incorporated into antisense oligonucleotides that showed antisenseactivity (Frieden et al., Nucleic Acids Research, 2003, 21, 6365-6372).

In certain embodiments, bicyclic nucleosides include, but are notlimited to, (A) α-L-Methyleneoxy (4′-CH₂—O-2′) BNA, (B) β-D-Methyleneoxy(4′-CH₂—O-2′) BNA, (C) Ethyleneoxy (4′-(CH₂)₂—O-2′) BNA, (D) Aminooxy(4′-CH₂—O—N(R)-2′) BNA, (E) Oxyamino (4′-CH₂—N(R)—O-2′) BNA, and (F)Methyl(methyleneoxy) (4′-CH(CH₃)—O-2′) BNA, (G) methylene-thio(4′-CH₂—S-2′) BNA, (H) methylene-amino (4′-CH2-N(R)-2′) BNA, (I) methylcarbocyclic (4′-CH₂—CH(CH₃)-2′) BNA, (J) propylene carbocyclic(4′-(CH₂)₃-2′) BNA, and (K) ethylene carbocyclic (4′-CH₂—CH₂-2) (carbaLNA or “cLNA”) as depicted below.

wherein Bx is the base moiety and R is independently H, a protectinggroup or C₁-C₁₂ alkyl.

In certain embodiments, bicyclic nucleoside having Formula I:

wherein:

Bx is a heterocyclic base moiety;

-Q_(a)-Q_(b)-Q_(c)- is —CH₂—N(R_(c))—CH₂—, —C(═O)—N(R_(c))—CH₂—,—CH₂—O—N(R_(c))—, —CH₂—N(R_(c))—O— or —N(R_(c))—O—CH₂;

R_(c) is C₁-C₁₂ alkyl or an amino protecting group; and

T_(a) and T_(b) are each, independently H, a hydroxyl protecting group,a conjugate group, a reactive phosphorus group, a phosphorus moiety or acovalent attachment to a support medium.

In certain embodiments, bicyclic nucleoside having Formula II:

wherein:

Bx is a heterocyclic base moiety;

T_(a) and T_(b) are each, independently H, a hydroxyl protecting group,a conjugate group, a reactive phosphorus group, a phosphorus moiety or acovalent attachment to a support medium;

Z_(a) is C₁-C₆ alkyl, C₂-C₆ alkenyl, C₂-C₆ alkynyl, substituted C₁-C₆alkyl, substituted C₂-C₆ alkenyl, substituted C₂-C₆ alkynyl, acyl,substituted acyl, substituted amide, thiol or substituted thio.

In one embodiment, each of the substituted groups, is, independently,mono or poly substituted with substituent groups independently selectedfrom halogen, oxo, hydroxyl, OJ_(c), NJ_(c)J_(d), SJ_(c), N₃,OC(═X)J_(c), and NJ_(e)C(═X)NJ_(c)J_(d), wherein each J_(c), J_(d) andJ_(e) is, independently, H, C₁-C₆ alkyl, or substituted C₁-C₆ alkyl andX is O or NJ_(c).

In certain embodiments, bicyclic nucleoside having Formula III:

wherein:

Bx is a heterocyclic base moiety;

T_(a) and T_(b) are each, independently H, a hydroxyl protecting group,a conjugate group, a reactive phosphorus group, a phosphorus moiety or acovalent attachment to a support medium;

Z_(b) is C₁-C₆ alkyl, C₂-C₆ alkenyl, C₂-C₆ alkynyl, substituted C₁-C₆alkyl, substituted C₂-C₆ alkenyl, substituted C₂-C₆ alkynyl orsubstituted acyl (C(═O)—).

In certain embodiments, bicyclic nucleoside having Formula IV:

wherein:

Bx is a heterocyclic base moiety;

T_(a) and T_(b) are each, independently H, a hydroxyl protecting group,a conjugate group, a reactive phosphorus group, a phosphorus moiety or acovalent attachment to a support medium;

R_(d) is C₁-C₆ alkyl, substituted C₁-C₆ alkyl, C₂-C₆ alkenyl,substituted C₂-C₆ alkenyl, C₂-C₆ alkynyl or substituted C₂-C₆ alkynyl;

each q_(a), q_(b), q_(c) and q_(d) is, independently, H, halogen, C₁-C₆alkyl, substituted C₁-C₆ alkyl, C₂-C₆ alkenyl, substituted C₂-C₆alkenyl, C₂-C₆ alkynyl or substituted C₂-C₆ alkynyl, C₁-C₆ alkoxyl,substituted C₁-C₆ alkoxyl, acyl, substituted acyl, C₁-C₆ aminoalkyl orsubstituted C₁-C₆ aminoalkyl;

In certain embodiments, bicyclic nucleoside having Formula V:

wherein:

Bx is a heterocyclic base moiety;

T_(a) and T_(b) are each, independently H, a hydroxyl protecting group,a conjugate group, a reactive phosphorus group, a phosphorus moiety or acovalent attachment to a support medium;

q_(a), q_(b), q_(e) and q_(f) are each, independently, hydrogen,halogen, C₁-C₁₂ alkyl, substituted C₁-C₁₂ alkyl, C₂-C₁₂ alkenyl,substituted C₂-C₁₂ alkenyl, C₂-C₁₂ alkynyl, substituted C₂-C₁₂ alkynyl,C₁-C₁₂ alkoxy, substituted C₁-C₁₂ alkoxy, OJ_(j), SJ_(j), SOJ_(j),SO₂J_(j), NJ_(j)J_(k), N₃, CN, C(═O)OJ_(j), C(═O)NJ_(j)J_(k),C(═O)J_(j), O—C(═O)NJ_(j)J_(k), N(H)C(═NH)NJ_(j)J_(k),N(H)C(═O)NJ_(j)J_(k) or N(H)C(═S)NJ_(j)J_(k);

or q_(e) and q_(f) together are ═C(q_(g))(q_(h));

q_(g) and q_(h) are each, independently, H, halogen, C₁-C₁₂ alkyl orsubstituted C₁-C₁₂ alkyl.

The synthesis and preparation of the methyleneoxy (4′-CH₂—O-2′) BNAmonomers adenine, cytosine, guanine, 5-methyl-cytosine, thymine anduracil, along with their oligomerization, and nucleic acid recognitionproperties have been described (Koshkin et al., Tetrahedron, 1998, 54,3607-3630). BNAs and preparation thereof are also described in WO98/39352 and WO 99/14226.

Analogs of methyleneoxy (4′-CH₂—O-2′) BNA, methyleneoxy (4′-CH₂—O-2′)BNA and 2′-thio-BNAs, have also been prepared (Kumar et al., Bioorg.Med. Chem. Lett., 1998, 8, 2219-2222). Preparation of locked nucleosideanalogs comprising oligodeoxyribonucleotide duplexes as substrates fornucleic acid polymerases has also been described (Wengel et al., WO99/14226). Furthermore, synthesis of 2′-amino-BNA, a novelcomformationally restricted high-affinity oligonucleotide analog hasbeen described in the art (Singh et al., J. Org. Chem., 1998, 63,10035-10039). In addition, 2′-Amino- and 2′-methylamino-BNA's have beenprepared and the thermal stability of their duplexes with complementaryRNA and DNA strands has been previously reported.

In certain embodiments, bicyclic nucleoside having Formula VI:

wherein:

Bx is a heterocyclic base moiety;

T_(a) and T_(b) are each, independently H, a hydroxyl protecting group,a conjugate group, a reactive phosphorus group, a phosphorus moiety or acovalent attachment to a support medium;

each q_(i), q_(j), q_(k) and q_(l) is, independently, H, halogen, C₁-C₁₂alkyl, substituted C₁-C₁₂ alkyl, C₂-C₁₂ alkenyl, substituted C₂-C₁₂alkenyl, C₂-C₁₂ alkynyl, substituted C₂-C₁₂ alkynyl, C₁-C₁₂ alkoxyl,substituted C₁-C₁₂ alkoxyl, OJ_(j), SJ_(j), SOJ_(j), SO₂J_(j),NJ_(j)J_(k), N₃, CN, C(═O)OJ_(j), C(═O)NJ_(j)J_(k), C(═O)J_(j),O—C(═O)NJ_(j)J_(k), N(H)C(═NH)NJ_(j)J_(k), N(H)C(═O)NJ_(j)J_(k) orN(H)C(═S)NJ_(j)J_(k); and

q_(i) and q_(j) or q_(l) and q_(k) together are ═C(q_(g))(q_(h)),wherein q_(g) and q_(h) are each, independently, H, halogen, C₁-C₁₂alkyl or substituted C₁-C₁₂ alkyl.

One carbocyclic bicyclic nucleoside having a 4′-(CH₂)₃-2′ bridge and thealkenyl analog bridge 4′-CH═CH—CH₂-2′ have been described (Frier et al.,Nucleic Acids Research, 1997, 25(22), 4429-4443 and Albaek et al., J.Org. Chem., 2006, 71, 7731-7740). The synthesis and preparation ofcarbocyclic bicyclic nucleosides along with their oligomerization andbiochemical studies have also been described (Srivastava et al., J. Am.Chem. Soc. 2007, 129(26), 8362-8379).

As used herein, “4′-2′ bicyclic nucleoside” or “4′ to 2′ bicyclicnucleoside” refers to a bicyclic nucleoside comprising a furanose ringcomprising a bridge connecting two carbon atoms of the furanose ringconnects the 2′ carbon atom and the 4′ carbon atom of the sugar ring.

As used herein, “monocylic nucleosides” refer to nucleosides comprisingmodified sugar moieties that are not bicyclic sugar moieties. In certainembodiments, the sugar moiety, or sugar moiety analogue, of a nucleosidemay be modified or substituted at any position.

As used herein, “2′-modified sugar” means a furanosyl sugar modified atthe 2′ position. In certain embodiments, such modifications includesubstituents selected from: a halide, including, but not limited tosubstituted and unsubstituted alkoxy, substituted and unsubstitutedthioalkyl, substituted and unsubstituted amino alkyl, substituted andunsubstituted alkyl, substituted and unsubstituted allyl, andsubstituted and unsubstituted alkynyl. In certain embodiments, 2′modifications are selected from substituents including, but not limitedto: O[(CH₂)_(n)O]_(m)CH₃, O(CH₂)_(n)NH₂, O(CH₂)_(n)CH₃, O(CH₂)_(n)ONH₂,OCH₂C(═O)N(H)CH₃, and O(CH₂)_(n)ON[(CH₂)_(n)CH₃]₂, where n and m arefrom 1 to about 10. Other 2′-substituent groups can also be selectedfrom: C₁-C₁₂ alkyl, substituted alkyl, alkenyl, alkynyl, alkaryl,aralkyl, O-alkaryl or O-aralkyl, SH, SCH₃, OCN, Cl, Br, CN, CF₃, OCF₃,SOCH₃, SO₂CH₃, ONO₂, NO₂, N₃, NH₂, heterocycloalkyl, heterocycloalkaryl,aminoalkylamino, polyalkylamino, substituted silyl, an RNA cleavinggroup, a reporter group, an intercalator, a group for improvingpharmacokinetic properties, or a group for improving the pharmacodynamicproperties of an antisense compound, and other substituents havingsimilar properties. In certain embodiments, modified nucleosidescomprise a 2′-MOE side chain (Baker et al., J. Biol. Chem., 1997, 272,11944-12000). Such 2′-MOE substitution have been described as havingimproved binding affinity compared to unmodified nucleosides and toother modified nucleosides, such as 2′-O-methyl, O-propyl, andO-aminopropyl. Oligonucleotides having the 2′-MOE substituent also havebeen shown to be antisense inhibitors of gene expression with promisingfeatures for in vivo use (Martin, P., Helv. Chim. Acta, 1995, 78,486-504; Altmann et al., Chimia, 1996, 50, 168-176; Altmann et al.,Biochem. Soc. Trans., 1996, 24, 630-637; and Altmann et al., NucleosidesNucleotides, 1997, 16, 917-926).

As used herein, a “modified tetrahydropyran nucleoside” or “modified THPnucleoside” means a nucleoside having a six-membered tetrahydropyran“sugar” substituted in for the pentofuranosyl residue in normalnucleosides (a sugar surrogate). Modified THP nucleosides include, butare not limited to, what is referred to in the art as hexitol nucleicacid (HNA), anitol nucleic acid (ANA), manitol nucleic acid (MNA) (seeLeumann, C J. Bioorg. & Med. Chem. (2002) 10:841-854), fluoro HNA(F-HNA) or those compounds having Formula X:

Formula X:

wherein independently for each of said at least one tetrahydropyrannucleoside analog of Formula X:

Bx is a heterocyclic base moiety;

T₃ and T₄ are each, independently, an internucleoside linking grouplinking the tetrahydropyran nucleoside analog to the antisense compoundor one of T₃ and T₄ is an internucleoside linking group linking thetetrahydropyran nucleoside analog to the antisense compound and theother of T₃ and T₄ is H, a hydroxyl protecting group, a linked conjugategroup or a 5′ or 3′-terminal group;

q₁, q₂, q₃, q₄, q₅, q₆ and q₇ are each independently, H, C₁-C₆ alkyl,substituted C₁-C₆ alkyl, C₂-C₆ alkenyl, substituted C₂-C₆ alkenyl, C₂-C₆alkynyl or substituted C₂-C₆ alkynyl; and

one of R₁ and R₂ is hydrogen and the other is selected from halogen,substituted or unsubstituted alkoxy, NJ₁J₂, SJ₁, N₃, OC(═X)J₁,OC(═X)NJ₁J₂, NJ₃C(═X)NJ₁J₂ and CN, wherein X is O, S or NJ₁ and each J₁,J₂ and J₃ is, independently, H or C₁-C₆ alkyl.

In certain embodiments, the modified THP nucleosides of Formula X areprovided wherein q_(m), q_(n), q_(p), q_(r), q_(s), q_(t) and q_(u) areeach H. In certain embodiments, at least one of q_(m), q_(n), q_(p),q_(r), q_(s), q_(t) and q_(u) is other than H. In certain embodiments,at least one of q_(m), q_(n), q_(p), q_(r), q_(s), q_(t) and q_(u) ismethyl. In certain embodiments, THP nucleosides of Formula X areprovided wherein one of R₁ and R₂ is F. In certain embodiments, R₁ isfluoro and R₂ is H; R₁ is methoxy and R₂ is H, and R₁ is methoxyethoxyand R₂ is H.

As used herein, “2′-modified” or “2′-substituted” refers to a nucleosidecomprising a sugar comprising a substituent at the 2′ position otherthan H or OH. 2′-modified nucleosides, include, but are not limited to,bicyclic nucleosides wherein the bridge connecting two carbon atoms ofthe sugar ring connects the 2′ carbon and another carbon of the sugarring; and nucleosides with non-bridging 2′ substituents, such as allyl,amino, azido, thio, O-allyl, O—C₁-C₁₀ alkyl, —OCF₃, O—(CH₂)₂—O—CH₃,2′-O(CH₂)₂SCH₃, O—(CH₂)₂—O—N(R_(m))(R_(n)), orO—CH₂—C(═O)—N(R_(m))(R_(n)), where each R_(m) and R_(n) is,independently, H or substituted or unsubstituted C₁-C₁₀ alkyl.2′-modified nucleosides may further comprise other modifications, forexample at other positions of the sugar and/or at the nucleobase.

As used herein, “2′-F” refers to a nucleoside comprising a sugarcomprising a fluoro group at the 2′ position.

As used herein, “2′-OMe” or “2′-OCH₃” or “2′-O-methyl” each refers to anucleoside comprising a sugar comprising an —OCH₃ group at the 2′position of the sugar ring.

As used herein, “MOE” or “2′-MOE” or “2′-OCH₂CH₂OCH₃” or“2′-O-methoxyethyl” each refers to a nucleoside comprising a sugarcomprising a —OCH₂CH₂OCH₃ group at the 2′ position of the sugar ring.

As used herein, “oligonucleotide” refers to a compound comprising aplurality of linked nucleosides. In certain embodiments, one or more ofthe plurality of nucleosides is modified. In certain embodiments, anoligonucleotide comprises one or more ribonucleosides (RNA) and/ordeoxyribonucleosides (DNA).

Many other bicyclo and tricyclo sugar surrogate ring systems are alsoknow in the art that can be used to modify nucleosides for incorporationinto antisense compounds (see for example review article: Leumann, J. C,Bioorganic & Medicinal Chemistry, 2002, 10, 841-854). Such ring systemscan undergo various additional substitutions to enhance activity.

Methods for the preparations of modified sugars are well known to thoseskilled in the art.

In nucleotides having modified sugar moieties, the nucleobase moieties(natural, modified or a combination thereof) are maintained forhybridization with an appropriate nucleic acid target.

In certain embodiments, antisense compounds comprise one or morenucleotides having modified sugar moieties. In certain embodiments, themodified sugar moiety is 2′-MOE. In certain embodiments, the 2′-MOEmodified nucleotides are arranged in a gapmer motif. In certainembodiments, the modified sugar moiety is a cEt. In certain embodiments,the cEt modified nucleotides are arranged throughout the wings of agapmer motif.

Modified Nucleobases

Nucleobase (or base) modifications or substitutions are structurallydistinguishable from, yet functionally interchangeable with, naturallyoccurring or synthetic unmodified nucleobases. Both natural and modifiednucleobases are capable of participating in hydrogen bonding. Suchnucleobase modifications may impart nuclease stability, bindingaffinity, increased selectivity for an allelic variant, or some otherbeneficial biological property to antisense compounds. Modifiednucleobases include synthetic and natural nucleobases such as, forexample, 5-methylcytosine (5-me-C). Certain nucleobase substitutions,including 5-methylcytosine substitutions, are particularly useful forincreasing the binding affinity of an antisense compound for a targetnucleic acid. For example, 5-methylcytosine substitutions have beenshown to increase nucleic acid duplex stability by 0.6-1.2° C. (Sanghvi,Y. S., Crooke, S. T. and Lebleu, B., eds., Antisense Research andApplications, CRC Press, Boca Raton, 1993, pp. 276-278).

Additional modified nucleobases include 5-hydroxymethyl cytosine,xanthine, hypoxanthine, 2-aminoadenine, 6-methyl and other alkylderivatives of adenine and guanine, 2-propyl and other alkyl derivativesof adenine and guanine, 2-thiouracil, 2-thiothymine and 2-thiocytosine,5-halouracil and cytosine, 5-propynyl (—C≡C—CH₃) uracil and cytosine andother alkynyl derivatives of pyrimidine bases, 6-azo uracil, cytosineand thymine, 5-uracil (pseudouracil), 4-thiouracil, 8-halo, 8-amino,8-thiol, 8-thioalkyl, 8-hydroxyl and other 8-substituted adenines andguanines, 5-halo particularly 5-bromo, 5-trifluoromethyl and other5-substituted uracils and cytosines, 7-methylguanine and7-methyladenine, 2-F-adenine, 2-amino-adenine, 8-azaguanine and8-azaadenine, 7-deazaguanine and 7-deazaadenine and 3-deazaguanine and3-deazaadenine.

Heterocyclic base moieties may also include those in which the purine orpyrimidine base is replaced with other heterocycles, for example7-deaza-adenine, 7-deazaguanosine, 2-aminopyridine and 2-pyridone.Nucleobases that are particularly useful for increasing the bindingaffinity of antisense compounds include 5-substituted pyrimidines,6-azapyrimidines and N-2, N-6 and O-6 substituted purines, including 2aminopropyladenine, 5-propynyluracil and 5-propynylcytosine.

In certain embodiments, antisense compounds comprise one or moremodified nucleobases. In certain embodiments, gap-widened antisenseoligonucleotides comprise one or more modified nucleobases. In certainembodiments, the modified nucleobase is 5-methylcytosine. In certainembodiments, each cytosine is a 5-methylcytosine.

Compositions and Methods for Formulating Pharmaceutical Compositions

Antisense oligonucleotides may be admixed with pharmaceuticallyacceptable active or inert substances for the preparation ofpharmaceutical compositions or formulations. Compositions and methodsfor the formulation of pharmaceutical compositions are dependent upon anumber of criteria, including, but not limited to, route ofadministration, extent of disease, or dose to be administered.

An antisense compound can be utilized in pharmaceutical compositions bycombining the antisense compound with a suitable pharmaceuticallyacceptable diluent or carrier. A pharmaceutically acceptable diluentincludes phosphate-buffered saline (PBS). PBS is a diluent suitable foruse in compositions to be delivered parenterally. Accordingly, in oneembodiment, employed in the methods described herein is a pharmaceuticalcomposition comprising an antisense compound and a pharmaceuticallyacceptable diluent. In certain embodiments, the pharmaceuticallyacceptable diluent is PBS. In certain embodiments, the antisensecompound is an antisense oligonucleotide.

Pharmaceutical compositions comprising antisense compounds encompass anypharmaceutically acceptable salts, esters, or salts of such esters, orany other oligonucleotide which, upon administration to an animal,including a human, is capable of providing (directly or indirectly) thebiologically active metabolite or residue thereof. Accordingly, forexample, the disclosure is also drawn to pharmaceutically acceptablesalts of antisense compounds, prodrugs, pharmaceutically acceptablesalts of such prodrugs, and other bioequivalents. Suitablepharmaceutically acceptable salts include, but are not limited to,sodium and potassium salts.

A prodrug can include the incorporation of additional nucleosides at oneor both ends of an antisense compound which are cleaved by endogenousnucleases within the body, to form the active antisense compound.

Conjugated Antisense Compounds

Antisense compounds may be covalently linked to one or more moieties orconjugates which enhance the activity, cellular distribution, increasedselectivity for an allelic variant, or cellular uptake of the resultingantisense oligonucleotides. Typical conjugate groups include cholesterolmoieties and lipid moieties. Additional conjugate groups includecarbohydrates, phospholipids, biotin, phenazine, folate, phenanthridine,anthraquinone, acridine, fluoresceins, rhodamines, coumarins, and dyes.

Antisense compounds can also be modified to have one or more stabilizinggroups that are generally attached to one or both termini of antisensecompounds to enhance properties such as, for example, nucleasestability. Included in stabilizing groups are cap structures. Theseterminal modifications protect the antisense compound having terminalnucleic acid from exonuclease degradation, and can help in deliveryand/or localization within a cell. The cap can be present at the5′-terminus (5′-cap), or at the 3′-terminus (3′-cap), or can be presenton both termini. Cap structures are well known in the art and include,for example, inverted deoxy abasic caps. Further 3′ and 5′-stabilizinggroups that can be used to cap one or both ends of an antisense compoundto impart nuclease stability include those disclosed in WO 03/004602published on Jan. 16, 2003.

Cell Culture and Antisense Compounds Treatment

The effects of antisense compounds on the level, activity or expressiontarget nucleic acids can be tested in vitro in a variety of cell types.Cell types used for such analyses are available from commercial vendors(e.g. American Type Culture Collection, Manassas, Va.; Zen-Bio, Inc.,Research Triangle Park, N.C.; Clonetics Corporation, Walkersville, Md.)and are cultured according to the vendor's instructions usingcommercially available reagents (e.g. Invitrogen Life Technologies,Carlsbad, Calif.). Illustrative cell types include, but are not limitedto, HepG2 cells, Hep3B cells, and primary hepatocytes. Illustrative celllines include GM04281, GM02171, and GM02173B cells.

In Vitro Testing of Antisense Oligonucleotides

Described herein are methods for treatment of cells with antisenseoligonucleotides, which can be modified appropriately for treatment withother antisense compounds.

In general, cells are treated with antisense oligonucleotides when thecells reach approximately 60-80% confluency in culture.

One reagent commonly used to introduce antisense oligonucleotides intocultured cells includes the cationic lipid transfection reagentLIPOFECTIN (Invitrogen, Carlsbad, Calif.). Antisense oligonucleotidesare mixed with LIPOFECTIN in OPTI-MEM 1 (Invitrogen, Carlsbad, Calif.)to achieve the desired final concentration of antisense oligonucleotideand a LIPOFECTIN concentration that typically ranges 2 to 12 ug/mL per100 nM antisense oligonucleotide.

Another reagent used to introduce antisense oligonucleotides intocultured cells includes LIPOFECTAMINE (Invitrogen, Carlsbad, Calif.).Antisense oligonucleotide is mixed with LIPOFECTAMINE in OPTI-MEM 1reduced serum medium (Invitrogen, Carlsbad, Calif.) to achieve thedesired concentration of antisense oligonucleotide and a LIPOFECTAMINEconcentration that typically ranges 2 to 12 ug/mL per 100 nM antisenseoligonucleotide.

Another technique used to introduce antisense oligonucleotides intocultured cells includes electroporation.

Cells are treated with antisense oligonucleotides by routine methods.Cells are typically harvested 16-24 hours after antisenseoligonucleotide treatment, at which time RNA or protein levels of targetnucleic acids are measured by methods known in the art and describedherein. In general, when treatments are performed in multiplereplicates, the data are presented as the average of the replicatetreatments.

The concentration of antisense oligonucleotide used varies from cellline to cell line. Methods to determine the optimal antisenseoligonucleotide concentration for a particular cell line are well knownin the art. Antisense oligonucleotides are typically used atconcentrations ranging from 1 nM to 300 nM when transfected withLIPOFECTAMINE. Antisense oligonucleotides are used at higherconcentrations ranging from 625 to 20,000 nM when transfected usingelectroporation.

RNA Isolation

RNA analysis can be performed on total cellular RNA or poly(A)+mRNA.Methods of

RNA isolation are well known in the art. RNA is prepared using methodswell known in the art, for example, using the TRIZOL Reagent(Invitrogen, Carlsbad, Calif.) according to the manufacturer'srecommended protocols.

Analysis of Inhibition of Target Levels or Expression

Reduction, inhibition, or expression of a target nucleic acid can beassayed in a variety of ways known in the art. For example, targetnucleic acid levels can be quantitated by, e.g., Northern blot analysis,competitive polymerase chain reaction (PCR), or quantitative real-timePCR. RNA analysis can be performed on total cellular RNA orpoly(A)+mRNA. Methods of RNA isolation are well known in the art.Northern blot analysis is also routine in the art. Quantitativereal-time PCR can be conveniently accomplished using the commerciallyavailable ABI PRISM 7600, 7700, or 7900 Sequence Detection System,available from PE-Applied Biosystems, Foster City, Calif. and usedaccording to manufacturer's instructions.

Quantitative Real-Time PCR Analysis of Target RNA Levels

Quantitation of target RNA levels may be accomplished by quantitativereal-time PCR using the ABI PRISM 7600, 7700, or 7900 Sequence DetectionSystem (PE-Applied Biosystems, Foster City, Calif.) according tomanufacturer's instructions. Methods of quantitative real-time PCR arewell known in the art.

Prior to real-time PCR, the isolated RNA is subjected to a reversetranscriptase (RT) reaction, which produces complementary DNA (cDNA)that is then used as the substrate for the real-time PCR amplification.The RT and real-time PCR reactions are performed sequentially in thesame sample well. RT and real-time PCR reagents are obtained fromInvitrogen (Carlsbad, Calif.). RT real-time-PCR reactions are carriedout by methods well known to those skilled in the art.

Gene (or RNA) target quantities obtained by real time PCR are normalizedusing either the expression level of a gene whose expression isconstant, such as cyclophilin A, or by quantifying total RNA usingRIBOGREEN (Invitrogen, Inc. Carlsbad, Calif.). Cyclophilin A expressionis quantified by real time PCR, by being run simultaneously with thetarget, multiplexing, or separately. Total RNA is quantified usingRIBOGREEN RNA quantification reagent (Invetrogen, Inc. Eugene, Oreg.).Methods of RNA quantification by RIBOGREEN are taught in Jones, L. J.,et al, (Analytical Biochemistry, 1998, 265, 368-374). A CYTOFLUOR 4000instrument (PE Applied Biosystems) is used to measure RIBOGREENfluorescence.

Probes and primers are designed to hybridize to target nucleic acids.Methods for designing real-time PCR probes and primers are well known inthe art, and may include the use of software such as PRIMER EXPRESSSoftware (Applied Biosystems, Foster City, Calif.).

Analysis of Protein Levels

Reduction, inhibition, or expression of target nucleic acids can beassessed by measuring target protein levels. Target protein levels canbe evaluated or quantitated in a variety of ways well known in the art,such as immunoprecipitation, Western blot analysis (immunoblotting),enzyme-linked immunosorbent assay (ELISA), quantitative protein assays,protein activity assays (for example, caspase activity assays),immunohistochemistry, immunocytochemistry or fluorescence-activated cellsorting (FACS). Antibodies directed to a target can be identified andobtained from a variety of sources, such as the MSRS catalog ofantibodies (Aerie Corporation, Birmingham, Mich.), or can be preparedvia conventional monoclonal or polyclonal antibody generation methodswell known in the art. Antibodies useful for the detection of mouse,rat, monkey, and human proteins are commercially available.

In Vivo Testing of Antisense Compounds

Antisense compounds, for example, antisense oligonucleotides, are testedin animals to assess their ability to selectively reduce or inhibitexpression of target gene product and produce phenotypic changes, suchas, amelioration of a disease symptom. Testing may be performed innormal animals, or in experimental disease models. For administration toanimals, antisense oligonucleotides are formulated in a pharmaceuticallyacceptable diluent, such as phosphate-buffered saline. Administrationincludes parenteral routes of administration, such as intraperitoneal,intravenous, and subcutaneous. Calculation of antisense oligonucleotidedosage and dosing frequency is within the abilities of those skilled inthe art, and depends upon factors such as route of administration andanimal body weight. Following a period of treatment with antisenseoligonucleotides, RNA or protein is isolated from tissue and changes intarget nucleic acid or protein expression are measured.

Administration

In certain embodiments, the compounds and compositions described hereinmay be administered in a number of ways depending upon whether local orsystemic treatment is desired and upon the area to be treated.Administration may be topical (including ophthalmic, vaginal, rectal,intranasal), oral, pulmonary (including by inhalation or insufflation ofpowders or aerosols, including by nebulizer; intratracheal, intranasal,epidermal and transdermal) or parenteral, for example, by intravenousdrip, intravenous injection or subcutaneous, intraperitoneal,intraocular, intravitreal, or intramuscular injection.

In certain embodiments, the compounds and compositions as describedherein are administered parenterally.

In certain embodiments, parenteral administration is by infusion.Infusion can be chronic or continuous or short or intermittent. Incertain embodiments, infused pharmaceutical agents are delivered with apump. In certain embodiments, parenteral administration is by injection.

In certain embodiments, compounds and compositions are delivered to theCNS. In certain embodiments, compounds and compositions are delivered tothe cerebrospinal fluid. In certain embodiments, compounds andcompositions are administered to the brain parenchyma. In certainembodiments, compounds and compositions are delivered to an animal byintrathecal administration, or intracerebroventricular administration.Broad distribution of compounds and compositions, described herein,within the central nervous system may be achieved with intraparenchymaladministration, intrathecal administration, or intracerebroventricularadministration.

In certain embodiments, parenteral administration is by injection. Theinjection may be delivered with a syringe or a pump. In certainembodiments, the injection is a bolus injection. In certain embodiments,the injection is administered directly to a tissue, such as striatum,caudate, cortex, hippocampus and cerebellum.

In certain embodiments, methods of specifically localizing apharmaceutical agent, such as by bolus injection, decreases medianeffective concentration (EC50) by a factor of 20, 25, 30, 35, 40, 45 or50. In certain embodiments, the pharmaceutical agent in an antisensecompound as further described herein. In certain embodiments, thetargeted tissue is brain tissue. In certain embodiments the targetedtissue is striatal tissue. In certain embodiments, decreasing EC50 isdesirable because it reduces the dose required to achieve apharmacological result in a patient in need thereof.

In certain embodiments, an antisense oligonucleotide is delivered byinjection or infusion once every month, every two months, every 90 days,every 3 months, every 6 months, twice a year or once a year.

Certain Compounds and Indications

Provided herein are compounds and methods that provide potent inhibitionand increased selectivity for a mutant allele. Potency is demonstratedby the percent inhibition of mutant mRNA achieved by the antisenseoligonucleotides targeting a SNP compared to the percent inhibition ofmutant mRNA achieved by the benchmark oligonucleotide. Selectivity isdemonstrated by the ability of the antisense oligonucleotide targeting aSNP to inhibit expression of the major allele or mutant allelepreferentially compared to the minor allele or wild type allele. Theusage of three cell lines with different genotypes at each SNP positionhave facilitated the determination of design rules that provide forpotent and selective SNP targeting antisense oligonucleotides.

In certain embodiments, the compounds are antisense oligonucleotides asfurther described herein. The antisense oligonucleotides preferentiallytarget a SNP or differentiating polymorphism. Oligonucleotides ofvarious lengths were tested and certain lengths were determined to bebeneficial for the targeting of SNPs.

In certain embodiments, the antisense oligonucleotides have a sequencethat is 12-30 nucleobases in length. In certain embodiments, theantisense oligonucleotides have a sequence that is 12-25 nucleobases inlength. In certain embodiments, the antisense oligonucleotides have asequence that is 12-21 nucleobases in length. In certain embodiments,the antisense oligonucleotides have a sequence that is 12-20 nucleobasesin length. In certain embodiments, the antisense oligonucleotides have asequence that is 13-20 nucleobases in length. In certain embodiments,the antisense oligonucleotides have a sequence that is 14-20 nucleobasesin length. In certain embodiments, the antisense oligonucleotides have asequence that is 15-20 nucleobases in length. In certain embodiments,the antisense oligonucleotides have a sequence that is 12-19 nucleobasesin length. In certain embodiments, the antisense oligonucleotides have asequence that is 13-19 nucleobases in length. In certain embodiments,the antisense oligonucleotides have a sequence that is 14-19 nucleobasesin length. In certain embodiments, the antisense oligonucleotides have asequence that is 15-19, nucleobases in length. In certain embodiments,the antisense oligonucleotides have a sequence that is 16-19 nucleobasesin length. In certain embodiments, the antisense oligonucleotides have asequence that is 17-19 nucleobases in length. In certain embodiments,the antisense oligonucleotides have a sequence that is 12, 13, 14, 15,16, 17, 18, 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 nucleobases inlength.

For oligonucleotides of various lengths, the position of the nucleosidecomplementary to the SNP position was shifted within the gap and thewings and the effect was tested. Certain positions within the antisenseoligonucleotide are shown to be beneficial for targeting SNPs.

In certain embodiments, the antisense oligonucleotide is at least 12, atleast 13, at least 14, at least 15, at least 16, at least 17 at least 18or at least 19 nucleobases in length and the SNP is complementary topositions 6-15 counting from the 5′ terminus of the antisenseoligonuceotide and/or positions 1-9 counting from the 5′ end of the gap.In certain embodiments, the antisense oligonucleotide is at least 12, atleast 13, at least 14, at least 15, at least 16, at least 17 at least 18or at least 19 nucleobases in length and the SNP is complementary topositions 8-14 counting from the 5′ terminus of the antisenseoligonuceotide and/or positions 1-9 counting from the 5′ end of the gap.In certain embodiments, the antisense oligonucleotide is at least 12, atleast 13, at least 14, at least 15, at least 16, at least 17 at least 18or at least 19 nucleobases in length and the SNP is complementary topositions 8-14 counting from the 5′ terminus of the antisenseoligonuceotide and/or positions 4-7 counting from the 5′ end of the gap.In certain embodiments, the antisense oligonucleotide is at least 12, atleast 13, at least 14, at least 15, at least 16, at least 17 at least 18or at least 19 nucleobases in length and the SNP is complementary topositions 8-10 counting from the 5′ terminus of the antisenseoligonuceotide and/or positions 4-6 counting from the 5′ end of the gap.

In certain embodiments, the SNP is complementary to position 8, 9, or 10counting from the 5′ terminus of the oligonucleotide or position 4, 5,or 6, counting from the 5′ end of the gap. For oligonucleotides ofvarious lengths, the effect of the length of the gap, 5′ wing, and 3′wing was tested.

Certain wing-gap-wing combinations were shown to be beneficial for a SNPtargeting antisense oligonucleotide. In certain embodiments the gap is7-11 nucleobases in length and each wing is independently 1-6nucleobases in length. In certain embodiments the gap is 7-11nucleobases in length and each wing is independently 2-6 nucleobases inlength. In certain embodiments the gap is 8-11 nucleobases in length andeach wing is independently 2-6 nucleobases in length. In certainembodiments the gap is 9-11 nucleobases in length and each wing isindependently 2-6 nucleobases in length. In certain embodiments the gapis 9 nucleobases in length and each wing is independently 2-6nucleobases in length. In certain embodiments the gap is 10 nucleobasesin length and each wing is independently 2-6 or 4-5 nucleobases inlength. In certain embodiments the gap is 11 nucleobases in length andeach wing is independently 2-6, or 4-5 nucleobases in length. In certainembodiments, the wing-gap-wing configuration is one of 4-7-4, 5-8-6,6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6, 6, 9, 2, 3-9-3,3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and 5-10-4.

For oligonucleotides of various lengths, the effect of certainchemistries was tested. Certain chemistry modifications were shown to bebeneficial for a SNP targeting antisense oligonucleotide. In certainembodiments, each nucleoside of each wing of the modified antisenseoligonucleotide has a 2′-MOE modification. In certain embodiments, eachnucleoside of each wing of the modified antisense oligonucleotide has ahigh affinity modification. In certain embodiments, the antisenseoligonucleotide is a mixed wing gapmer. In such embodiment, themodifications and combination of modifications at the 3′ wing and at the5′ wing may be the same or they may be different. In certainembodiments, the antisense oligonucleotide has one or more 2′-MOEmodifications in the wings and/or one or more high affinitymodifications in the wings. In certain embodiments, the high affinitymodification is a cEt modification. In certain embodiments, theantisense oligonucleotide has a high affinity modification at positions2, 3, 13, and 14 of the antisense oligonucleotide (counting from the 5′terminus). In certain embodiments, the antisense oligonuceotide has one,two, three, or four high affinity modifications in at least one of thewings. In certain embodiments, the antisense oligonuceotide has one,two, three, or four high affinity modifications in each of the 5′ and 3′wings independently. In certain embodiments, the antisenseoligonucleotide has a high affinity modification at positions 2 and 3 inone or both of the 5′ and 3′ wings (counting from the 5′ terminus of the5′ wing and the 3′ terminus of the 3′ wing). In certain embodiments, theantisense oligonuceotide has a high affinity modification at positions2, 3 and 4 in one or both of the 5′ and 3′ wings (counting from the 5′terminus of the 5′ wing and the 3′ terminus of the 3′ wing,). In certainembodiments, the antisense oligonuceotide has a high affinitymodification at positions 1 of the 5′ and/or 3′ wings (counting from the5′ terminus of the 5′ wing and the 3′ terminus of the 3′ wing,). Incertain embodiments, the antisense oligonuceotide has a high affinitymodification at positions 1 of the 5′ and 3′ wings (counting from the 5′terminus of the 5′ wing and the 3′ terminus of the 3′ wing,) and atleast one other position in the wing. In certain embodiments, theantisense oligonuceotide has alternating 2′-MOE and high affinitymodification in at least one of the 5′ and 3′ wings.

In certain embodiments, the compound comprises an antisenseoligonucleotide incorporating one or more of the design rules providedabove.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 12 to 30 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 6-15 begining from the 5′ terminus of the antisenseoligonuceotide or positions 1-9 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments the single nucleotide polymorphism site contains adifferentiating polymorphism. In certain embodiments, the singlenucleotide polymorphism site is on a mutant allele. In certainembodiments, the mutant allele is associated with disease. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 12 to 20 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 6-15 begining from the 5′ terminus of the antisenseoligonuceotide or positions 1-9 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 12 to 20 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 8-14 begining from the 5′ terminus of the antisenseoligonuceotide or positions 1-9 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 12 to 20 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 8-14 begining from the 5′ terminus of the antisenseoligonuceotide or positions 4-7 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 12 to 20 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 8-10 begining from the 5′ terminus of the antisenseoligonuceotide or positions 4-6 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 12 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 8-10 begining from the 5′ terminus of the antisenseoligonuceotide or positions 4-6 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 13 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 8-10 begining from the 5′ terminus of the antisenseoligonuceotide or positions 4-6 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 14 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 8-10 beginning from the 5′ terminus of the antisenseoligonuceotide or positions 4-6 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 15 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 6-15 beginning from the 5′ terminus of the antisenseoligonuceotide or positions 1-9 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 15 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein the single nucleotide polymorphism aligns with any one ofpositions 8-10 beginning from the 5′ terminus of the antisenseoligonuceotide or positions 4-6 begining from the 5′ end of the gap ofthe modified antisense oligonucleotide; and wherein each nucleoside ofeach wing has a modified sugar or sugar surrogate. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 4-7-4, 5-8-6, 6-8-5, 6-7-6, 5-7-5. 6-8-5, 5-8-6, 3-9-4, 4-9-3, 2-9-6,6, 9, 2, 3-9-3, 3-9-5, 5-9-3, 5-9-4, 4-9-5, 5-9-5, 4-11-4, 4-10-5 and5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 15 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 6, 8, 9, 10, 11, or 14 beginning from the 5′ terminusof the modified antisense oligonucleotide aligns with the singlenucleotide polymorphism; and wherein each nucleoside of each wingsegment modified sugar or sugar surrogate. In certain embodiments, thewing-gap-wing motif is any one of the group consisting of 2-9-6, 3-9-3,3-9-5, 4-9-5, 4-11-4, and 5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 15 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 1, 4, 5, 6, 7, or 9 of the gap segment aligns with thesingle nucleotide polymorphism; and wherein each nucleoside of each wingsegment has a modified sugar or sugar surrogate. In certain embodiments,the wing-gap-wing motif is any one of the group consisting of 2-9-6,3-9-3, 3-9-5, 4-9-5, 4-11-4, and 5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 15 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 6, 7, 8, 9, 10, 11, or 12 of the modified antisenseoligonucleotide aligns with the single nucleotide polymorphism; andpositions 2 and 3 of the 5′ and 3′ wing segments comprise a4′-CH(CH₃)—O-2′ bridge. In certain embodiments, the wing-gap-wing motifis any one of the group consisting of 2-9-6, 3-9-3, 3-9-5, 4-9-5,4-11-4, and 5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 15 to 19 linked nucleosides and fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 3, 4, 5, 6, 7, 8 or 9 of the gap segment aligns withthe single nucleotide polymorphism; and positions 2 and 3 of the 5′ and3′ wing segments comprise a 4′-CH(CH₃)—O-2′ bridge. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 2-9-6, 3-9-3, 3-9-5, 4-9-5, 4-11-4, and 5-10-4.

A compound comprising a modified antisense oligonucleotide consisting of15 to 19 linked nucleosides and fully complementary to a singlenucleotide polymorphism site, wherein the modified antisenseoligonucleotide comprises a wing-gap-wing motif, wherein position 6, 7,8, 9, 10, 11, or 12 of the modified antisense oligonucleotide alignswith the single nucleotide polymorphism; and positions 2, 3, 13, and 14of the antisense oligonucleotide comprise a 4′-CH(CH₃)—O-2′ bridge. Incertain embodiments, the wing-gap-wing motif is any one of the groupconsisting of 2-9-6, 3-9-3, 3-9-5, 4-9-5, 4-11-4, and 5-10-4.

A compound comprising a modified antisense oligonucleotide consisting of15 to 19 linked nucleosides and fully complementary to a singlenucleotide polymorphism site, wherein the modified antisenseoligonucleotide comprises a wing-gap-wing motif, wherein position 3, 4,5, 6, 7, 8, or 9 of the gap segment aligns with the single nucleotidepolymorphism; and positions 2, 3, 13, and 14 of the antisense antisenseoligonucleotide comprise a 4′-CH(CH₃)—O-2′ bridge. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 2-9-6, 3-9-3, 3-9-5, 4-9-5, 4-11-4, and 5-10-4.

In certain embodiments, the compound comprise a modified antisenseoligonucleotide consisting of 17 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 8, 9, or 10 of the modified antisense oligonucleotidealigns with the single nucleotide polymorphism; and wherein eachnucleoside of each wing segment comprises a 2′-O-methoxyethyl sugar. Incertain embodiments, the wing-gap-wing motif is any one of the groupconsisting of 2-9-6, 3-9-3, 3-9-5, 4-9-5, 4-11-4, and 5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 17 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 5, 6, or 7 of the gap segment aligns with the singlenucleotide polymorphism; and wherein each nucleoside of each wingsegment comprises a 2′-O-methoxyethyl sugar. In certain embodiments, thewing-gap-wing motif is any one of the group consisting of 2-9-6, 3-9-3,3-9-5, 4-9-5, 4-11-4, and 5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 17 to 19 linked nucleosides, fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 8, 9, or 10 of the modified antisense oligonucleotidealigns with the single nucleotide polymorphism; and positions 2 and 3 ofthe 5′ and 3′ wing segments comprise a 4′-CH(CH₃)—O-2′ bridge. Incertain embodiments, the wing-gap-wing motif is any one of the groupconsisting of 2-9-6, 3-9-3, 3-9-5, 4-9-5, 4-11-4, and 5-10-4.

In certain embodiments, the compound comprises a modified antisenseoligonucleotide consisting of 17 to 19 linked nucleosides and fullycomplementary to a single nucleotide polymorphism site, wherein themodified antisense oligonucleotide comprises a wing-gap-wing motif,wherein position 5, 6, or 7 of the gap segment aligns with the singlenucleotide polymorphism; and positions 2 and 3 of the 5′ and 3′ wingsegments comprise a 4′-CH(CH₃)—O-2′ bridge. In certain embodiments, thewing-gap-wing motif is any one of the group consisting of 2-9-6, 3-9-3,3-9-5, 4-9-5, 4-11-4, and 5-10-4.

A compound comprising a modified antisense oligonucleotide consisting of17 to 19 linked nucleosides and fully complementary to a singlenucleotide polymorphism site, wherein the modified antisenseoligonucleotide comprises a wing-gap-wing motif, wherein position 8, 9,or 10 of the modified oligonucleotide aligns with the single nucleotidepolymorphism; and positions 2, 3, 13, and 14 of the antisense antisenseoligonucleotide comprise a 4′-CH(CH₃)—O-2′ bridge. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 2-9-6, 3-9-3, 3-9-5, 4-9-5, 4-11-4, and 5-10-4.

A compound comprising a modified antisense oligonucleotide consisting of17 to 19 linked nucleosides and fully complementary to a singlenucleotide polymorphism site, wherein the modified antisenseoligonucleotide comprises a wing-gap-wing motif, wherein position 5, 6,or 7 of the gap segment aligns with the single nucleotide polymorphism;and positions 2, 3, 13, and 14 of the antisense oligonucleotide comprisea 4′-CH(CH₃)—O-2′ bridge. In certain embodiments, the wing-gap-wingmotif is any one of the group consisting of 2-9-6, 3-9-3, 3-9-5, 4-9-5,4-11-4, and 5-10-4.

In a certain embodiment, the antisense oligonucleotide is 11 to 20linked nucleosides in length and has, independently, 2 to 5 linkednucleosides in the 5′ and 3′ wings and 7 to 11 linked nucleosides in thegap. The SNP is complementary to position 5, 6, 7, 8, 9, 10, 11, 12, 13,or 14 of the antisense oligonucleotide (counting from the 5′ terminus ofthe antisense oligonucleotide) or position 1, 2, 3, 4, 5, 6, 7, 8, 9, or10 counting from the 5′ terminus of the gap segment.

In a certain embodiment, the antisense oligonucleotide is 15 to 19linked nucleosides in length and has, independently, 2 to 5 linkednucleosides in the 5′ and 3′ wings and 7 to 11 linked nucleosides in thegap. The SNP is complementary to position 6, 7, 8, 9, or 10 of theantisense oligonucleotide (counting from the 5′ terminus of theantisense oligonucleotide) or position 4, 5, 6, or 7 counting from the5′ terminus of the gap segment.

In a certain embodiment, the antisense oligonucleotide is 17 linkednucleosides in length and has, independently, 2 to 5 linked nucleosidesin the 5′ and 3′ wing segments and 9 to 11 linked nucleosides in the gapsegment. The SNP is complementary to position 8, 9, or 10 of theantisense oligonucleotide (counting from the 5′ terminus of theantisense oligonucleotide) or position 5, 6, or 7 (counting from the 5′terminus of the gap segment).

In a certain embodiment, the antisense oligonucleotide is 18 linkednucleosides in length and has, independently, 2 to 5 linked nucleosidesin the 5′ and 3′ wing segments and 9 to 11 linked nucleosides in the gapsegment. The SNP is complementary to position 8, 9, or 10 of theantisense oligonucleotide (counting from the 5′ terminus of theantisense oligonucleotide) or position 5, 6, or 7 (counting from the 5′terminus of the gap segment).

In a certain embodiment, the antisense oligonucleotide is 19 linkednucleosides in length and has, independently, 2 to 5 linked nucleosidesin the 5′ and 3′ wing segments and 9 to 11 linked nucleosides in the gapsegment. The SNP is complementary to position 8, 9, or 10 of theantisense oligonucleotide (counting from the 5′ terminus of theantisense oligonucleotide) or position 5, 6, or 7 (counting from the 5′terminus of the gap segment).

In certain, the antisense oligonucleotide is any of those listed in theexamples. In certain embodiments, they are designated by sequence andchemistry as reflected by the ISIS number. In certain embodiments, theantisense oligonucleotide is any of those listed in the examples ashaving at least, about, greater than about or greater than 50%, 55%,60%, 65%, 70%, 75%, 80%, 85%, 90% or 95% inhibition of a mutantHuntingtin allele.

In certain embodiments, the antisense oligonucleotide is any of thoselisted in the examples as selectively inhibiting the expression of themutant huntingtin allele at least, about, greater than about or greaterthan 2 fold, 3 fold, 4 fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold or10 fold over the expression of a wild-type huntingtin allele or by atleast 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80% compared to expressionof a wild-type huntingtin allele.

In certain embodiments, the invention provides methods of treating anindividual comprising administering one or more pharmaceuticalcompositions described herein. In certain embodiments, the individualhas an allelic variant associated with a disease or disorder. Thepharmaceutical compositions provided herein preferentially target a SNP.In certain embodiments, the SNP is a differentiating polymorphism.

Methods have been described for determining whether a SNP is specific toa disease associated allele and more specifically whether a SNP variantof an allele of a heterozygous patient is on the same allele as adisease-causing mutation that is at a remote region of the gene's mRNA(WO 2008/147930 and WO 2008/143774).

In certain embodiments, the disease is a neurodegenerative disorder. Incertain embodiments, the neurodegenerative disorder is Huntington'sDisease. In certain embodiments, the targeted SNP is one or more of:rs6446723, rs3856973, rs2285086, rs363092, rs916171, rs6844859,rs7691627, rs4690073, rs2024115, rs11731237, rs362296, rs10015979,rs7659144, rs363096, rs362273, rs16843804, rs362271, rs362275,rs3121419, rs362272, rs3775061, rs34315806, rs363099, rs2298967,rs363088, rs363064, rs363102, rs2798235, rs363080, rs363072, rs363125,rs362303, rs362310, rs10488840, rs362325, rs35892913, rs363102,rs363096, rs11731237, rs10015979, rs363080, rs2798235, rs1936032,rs2276881, rs363070, rs35892913, rs12502045, rs6446723, rs7685686,rs3733217, rs6844859, rs362331, rs1143646, rs2285086, rs2298969,rs4690072, rs916171, rs3025849, rs7691627, rs4690073, rs3856973,rs363092, rs362310, rs362325, rs363144, rs362303, rs34315806, rs363099,rs363081, rs3775061, rs2024115, rs10488840, rs363125, rs362296,rs2298967, rs363088, rs363064, rs362275, rs3121419, rs3025849, rs363070,rs362273, rs362272, rs362306, rs362271, rs363072, rs16843804, rs7659144,rs363120, and rs12502045. In certain embodiments the compounds areISIS460065, ISIS 459978, ISIS 460028, ISIS 460209, ISIS 460208, and ISIS460206.

In certain embodiments, described herein, are methods of selectivelyreducing the expression of a mutant huntingtin allele in a cell, tissueor animal by administering to the cell, tissue, or animal a compound asdescribed herein. In certain embodiments, the compound comprises amodified oligonucleotide complementary to the mutant huntingtin allele.In certain embodiments, the modified oligonucleotide is complementary tothe mutant huntingtin allele at a position comprising a singlenucleotide polymorphism described herein. In certain embodiments, thesingle nucleotide polymorphism is selected from those described herein.In certain embodiments, the single nucleotide polymorphism is selectedfrom rs362306, rs362331, rs2298969, rs7685686, rs4690072, rs2024115 orrs363088.

In certain embodiments, described herein, are methods of treating,ameliorating or slowing the onset or progression of Huntington's Diseaseby administering to an animal a compound described herein. In certainembodiment the compound comprises a modified oligonucleotidecomplementary to a mutant huntingtin allele at a position on the allelecomprising a single nucleotide polymorphism described herein. In certainembodiments, the single nucleotide polymorphism is selected fromrs362306, rs362331, rs2298969, rs7685686, rs4690072, rs2024115 orrs363088. In certain embodiments, the compound administered to theanimal treats, ameliorates or slows the onset or progressionHuntington's Disease by selectively reducing the mutant huntingtinallele.

In certain embodiments the expression of the mutant huntingtin allele isselectively reduced at least, about or greater than 2 fold, 3 fold, 4fold, 5 fold, 6 fold, 7 fold, 8 fold, 9 fold or 10 fold over theexpression of a wild-type huntingtin allele or by at least 40%, 45%,50%, 55%, 60%, 65%, 70%, 75%, 80% compared to expression of a wild-typehuntingtin allele.

In certain embodiments, one or more additional compounds comprising amodified oligonucleotide complementary to a mutant huntingtin allele ata position comprising a single nucleotide polymorphism site selectedfrom rs362306, rs362331, rs2298969, rs7685686, rs4690072, rs2024115 orrs363088 is administered.

In certain embodiments, the modified oligonucleotide has a specificlength, gap, wing, chemistry and SNP position alignment as furtherdescribed herein. In certain embodiments, the modified oligonucleotideconsists of 15 to 20 linked nucleosides and position 5, 6, 7, 8, 9, 10,11, 12, 13, 14, or 15 of the modified oligonucleotide, as counted fromthe 5′ terminus of the modified oligonucleotide, aligns with the singlenucleotide polymorphism.

In certain embodiments, the modified oligonucleotide is 90%complementary to the mutant huntingtin allele.

The method of claim 40, wherein the modified oligonucleotide is 95%complementary to the mutant huntingtin allele.

The method of claim 40, wherein the modified oligonucleotide is 100%complementary to the mutant huntingtin allele.

In certain embodiments, described herein are methods for determiningwhether an agent selectively inhibits expression of a first allelicvariant of a gene relative to a second allelic variant of the genewherein the first allelic variant comprises a first nucleotide at a SNPposition and the second allelic variant comprises a second nucleotide atthe SNP position, comprising:

-   (i) contacting a first cell, tissue or animal with the agent at one    or more concentrations, wherein the first cell, tissue or animal is    homozygous for the first nucleotide at the SNP position;-   (ii) contacting a second cell, tissue or animal with the agent at    one or more concentrations, wherein the second cell line is    homozygous for the second nucleotide at the SNP position or is    heterozygous for the first and the second nucleotides at the SNP    position;-   (iii) measuring inhibition of expression of the allelic variant in    each of the cell, tissue or animal for each of the one or more    concentrations of the agent; and-   (iv) comparing the inhibition of expression in each of the cell,    tissue or animal for each of the one or more concentrations of the    agent to determine whether the agent selectively inhibits expression    of the first allelic variant of the gene relative to the second    allelic variant of the gene.

In certain embodiments, the methods described herein further comprisethe step of contacting a third cell, tissue or animal with the agent atone or more concentrations, wherein the third cell, tissue or animal ishomozygous for the second nucleotide at the SNP position or isheterozygous for the first and the second nucleotides at the SNPposition and has a different genotype than the first cell, tissue oranimal and the second cell, tissue or animal.

In certain embodiments, the cell, tissue or animal is a YAC18 cell,tissue or mouse, a BACHD cell, tissue or mouse, a cell line derived froma human Huntington's Disease patient or any combination thereof.

In certain embodiments, the first allelic variant is a mutant allele andthe second allelic variant is a wild-type allele. In certainembodiments, the first allelic variant is a wild-type allele and thesecond allelic variant is a mutant allele.

In certain embodiments, the mutant allelic variant is associated withdisease. In certain embodiments, the disease is the result of a gain oftoxic function. In certain embodiments, the disease is Huntington'sDisease.

In certain embodiments, the first nucleotide is in linkagedisequilibrium with a disease associated mutation. In certainembodiments, the second nucleotide is in linkage disequilibrium with adisease associated mutation. In certain embodiments, the diseaseassociated mutation is a tri-nucleotide repeat expansion. In certainembodiments, the tri-nucleotide repeat expansion is a CAG expansion. Incertain embodiments, the CAG expansion is in a HTT gene.

In certain embodiments, the first nucleotide is A and the secondnucleotide is any of C, G, or T. In certain embodiments, the firstnucleotide is C and the second nucleotide is any of A, G, or T. Incertain embodiments, the first nucleotide is G and the second nucleotideis any of A, C, or T. In certain embodiments, the first nucleotide is Tand the second nucleotide is any of A, C, or G. In certain embodiments,the first nucleotide is in linkage disequilibrium with a diseaseassociated mutation. In certain embodiments, the disease associatedmutation is a CAG expansion. In certain embodiments, the CAG expansionis in a HTT gene.

In certain embodiments, the agent is an antisense compound. In certainembodiments, the antisense compound is an antisense oligonucleotide. Incertain embodiments, the antisense oligonucleotide is a gapmer. Incertain embodiments, the gapmer has a wing-gap-wing motif. In certainembodiments, the wing-gap-wing motif is any one of the group consistingof 5-10-5, 2-9-6, 3-9-3, 3-9-4, 3-9-5, 4-7-4, 4-9-3, 4-9-4, 4-9-5,4-10-5, 4-11-4, 4-11-5, 5-7-5, 5-8-6, 5-9-3, 5-9-5, 5-10-4, 5-10-5,6-7-6, 6-8-5, and 6-9-2. In certain embodiments, at least oneinternucleoside linkage is a modified internucleoside linkage. Incertain embodiments, each internucleoside linkage is a phosphorothioateinternucleoside linkage. In certain embodiments, at least one nucleosidecomprises a modified nucleobase. In certain embodiments, the modifiednucleobase is a 5′-methylcytosine. In certain embodiments, at least onenucleoside of at least one of the wing regions comprises a modifiedsugar or sugar surrogate. In certain embodiments, each of thenucleosides of each wing region comprises a modified sugar or sugarsurrogate. In certain embodiments, the sugar or sugar surrogate is a2′-O-methoxyethyl modified sugar. In certain embodiments, at least oneof the wing regions comprises a 4′ to 2′ bicyclic nucleoside and atleast one of the remaining wing nucleosides is a non-bicyclic2′-modified nucleoside. In certain embodiments, the non-bicyclic2′-modified nucleoside is a 2′-O-methoxyethyl nucleoside. In certainembodiments, the 4′ to 2′ bicyclic nucleoside is 4′-CH(CH3)-O-2′bicyclic nucleoside.

In certain embodiments, the first cell line is any of the groupconsisting of fibroblast cell line, neuronal cell line, or lymphoblastcell line. In certain embodiments, the second cell line is any of thegroup consisting of fibroblast cell line, neuronal cell line, orlymphoblast cell line. In certain embodiments, the third cell line isany of the group consisting of fibroblast cell line, neuronal cell line,or lymphoblast cell line. In certain embodiments, the fibroblast cellline is any of the group consisting of GM04281, GM02171, and GM02173B.In certain embodiments, the neuronal cell line comprises a cell linederived from a YAC18 mouse, a cell line derived from a BACHD mouse, or acell line derived from a human Huntington's Disease patient.

In certain embodiments, the SNP position is any of the group consistingof rs6446723, rs3856973, rs2285086, rs363092, rs916171, rs6844859,rs7691627, rs4690073, rs2024115, rs11731237, rs362296, rs10015979,rs7659144, rs363096, rs362273, rs16843804, rs362271, rs362275,rs3121419, rs362272, rs3775061, rs34315806, rs363099, rs2298967,rs363088, rs363064, rs363102, rs2798235, rs363080, rs363072, rs363125,rs362303, rs362310, rs10488840, rs362325, rs35892913, rs363102,rs363096, rs11731237, rs10015979, rs363080, rs2798235, rs1936032,rs2276881, rs363070, rs35892913, rs12502045, rs6446723, rs7685686,rs3733217, rs6844859, rs362331, rs1143646, rs2285086, rs2298969,rs4690072, rs916171, rs3025849, rs7691627, rs4690073, rs3856973,rs363092, rs362310, rs362325, rs363144, rs362303, rs34315806, rs363099,rs363081, rs3775061, rs2024115, rs10488840, rs363125, rs362296,rs2298967, rs363088, rs363064, rs362275, rs3121419, rs3025849, rs363070,rs362273, rs362272, rs362306, rs362271, rs363072, rs16843804, rs7659144,rs363120, and rs12502045.

In certain embodiments, the one or more concentration is any of thegroup consisting of two concentrations, three concentrations, fourconcentrations, and five concentrations.

In certain embodiments, selectivity is determined by increasedinhibition of the allelic variant in one cell line as compared toanother cell line.

In certain embodiments, described herein are methods for determiningwhether an agent selectively inhibits expression of a first allelicvariant of a gene relative to a second allelic variant of the genewherein the first allelic variant comprises a first nucleotide at a SNPposition and the second allelic variant comprises a second nucleotide atthe SNP position, comprising:

-   (i) contacting a first cell line with the agent at one or more    concentrations, wherein the first cell line is homozygous for the    first nucleotide at the SNP position;-   (ii) contacting a second cell line with the agent at one or more    concentrations, wherein the second cell line is homozygous for the    second nucleotide at the SNP position or is heterozygous for the    first and the second nucleotides at the SNP position;-   (iii) measuring inhibition of expression of the allelic variant in    each of the cell lines for each of the one or more concentrations of    the agent; and-   (iv) comparing the inhibition of expression in each of the cell    lines for each of the one or more concentrations of the agent to    determine whether the agent selectively inhibits expression of the    first allelic variant of the gene relative to the second allelic    variant of the gene.-   (v) calculating IC50 values for the agent in each of the first cell    line, the second cell line, and the third cell line; and-   (vi) comparing the IC50 values to determine whether the agent    selectively inhibits expression of the first allelic variant or    mutant allele.

In certain embodiments, the huntingtinSNP targeting oligonucleotidesprovided herein are selective for the mutant allele and demonstrate apotency of within at least 10-fold, at least 9-fold, at least 8-fold, atleast 7-fold, at least 6-fold, at least 5-fold, at least 4-fold, atleast 3-fold or at least 2-fold of the potency of the benchmark PAN ASO,ISIS 387916. In certain embodiments, the SNP targeting oligonucleotidesprovided herein have an IC50 in a cell line homozygous for the mutantallele of no more than 6.5, 6, 5.5, 5, 4.5, 4, 3.5, 3, 2.5, 2, 1.5, 1 or0.75. In certain embodiments, the SNP targeting oligonucleotidesprovided herein have an IC50 in a cell line that is homozygous for thewild-type allele that is at least 2 fold, 3 fold, 4 fold or 5 fold, 6fold, 7 fold, 8 fold, 9 fold or 10 fold less than the IC50 in a cellline homozygous for the mutant allele. In certain embodiments, the SNPtargeting oligonucleotides provided herein have an IC50 in a cell linethat is heterozygous for the mutant and wild-type allele that is atleast 1.4 fold, 1.7 fold, 2 fold, 2.2, 2.4, 2.5, 2.6, 2.8 or 3 fold lessthan the IC50 in a cell line homozygous for the mutant allele. Incertain embodiments, the SNP targeting oligonucleotides include or areselected from ISIS Nos 435869, 460069, 460206, 460019, 460071, 460207,460212, 460218, 460231, 474892, 460012, 460208, 435879, 460065, 460085,460209, 474871, 474891, 474919, 474923, 476333, 476337, 435890, 460026,460210, 463571, 476444.

Therapeutically Effective Dosages

In certain embodiments, administration of a therapeutically effectiveamount of an antisense compound targeted to the mutant huntingtin alleleis accompanied by monitoring of expression of a gene product in anindividual, to determine an individual's response to administration ofthe antisense compound. In certain embodiments, the gene product ishuntingtin mRNA or protein. An individual's response to administrationof the antisense compound is used by a physician to determine the amountand duration of therapeutic intervention.

In certain embodiments, administration of an antisense compound targetedto a mutant nucleic acid results in reduction of mRNA or proteinexpression by at least 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70,75, 80, 85, 90, 95 or 99%, or a range defined by any two of thesevalues. In certain embodiments, the mutant nucleic acid is huntingtinnucleic acid, the mRNA is huntingtin mRNA, and the protein is huntingtinprotein.

In certain embodiments, pharmaceutical compositions comprising anantisense compound targeted to a mutant allele are used for thepreparation of a medicament for treating a patient suffering orsusceptible to Huntington's Disease.

Certain Combination Therapies

In certain embodiments, one or more pharmaceutical compositions of thepresent invention are co-administered with one or more otherpharmaceutical agents. In certain embodiments, such one or more otherpharmaceutical agents are designed to treat the same disease, disorder,or condition as the one or more pharmaceutical compositions of thepresent invention. In certain embodiments, such one or more otherpharmaceutical agents are designed to treat a different disease,disorder, or condition as the one or more pharmaceutical compositions ofthe present invention. In certain embodiments, such one or more otherpharmaceutical agents are designed to treat an undesired side effect ofone or more pharmaceutical compositions of the present invention. Incertain embodiments, one or more pharmaceutical compositions of thepresent invention are co-administered with another pharmaceutical agentto treat an undesired effect of that other pharmaceutical agent. Incertain embodiments, one or more pharmaceutical compositions of thepresent invention are co-administered with another pharmaceutical agentto produce a combinational effect. In certain embodiments, one or morepharmaceutical compositions of the present invention are co-administeredwith another pharmaceutical agent to produce a synergistic effect.

In certain embodiments, one or more pharmaceutical compositions of thepresent invention and one or more other pharmaceutical agents areadministered at the same time. In certain embodiments, one or morepharmaceutical compositions of the present invention and one or moreother pharmaceutical agents are administered at different times. Incertain embodiments, one or more pharmaceutical compositions of thepresent invention and one or more other pharmaceutical agents areprepared together in a single formulation. In certain embodiments, oneor more pharmaceutical compositions of the present invention and one ormore other pharmaceutical agents are prepared separately.

EXAMPLES Non-Limiting Disclosure and Incorporation by Reference

While certain compounds, compositions and methods described herein havebeen described with specificity in accordance with certain embodiments,the following examples serve only to illustrate the compounds describedherein and are not intended to limit the same. Each of the patents,applications, printed publications, and other published documentsmentioned or referred to in this specification are herein incorporatedby reference in their entirety.

Example 1 Single Nucleotide Polymorphisms (SNPs) in the Huntingtin (HTT)Gene Sequence

The HTT genomic sequence, designated herein as SEQ ID NO: 1(NT_(—)006081.18 truncated from nucleotides 1566000 to 1768000) wasaligned with the HTT mRNA, designated herein as SEQ ID NO: 2(NM_(—)002111.6), using the EMBL-EBI sequence database (ClustalW2,http://www.ebi.ac.uk/Tools/clustalw2/index.html), and the output ispresented in FIG. 1. SNP positions (identified by Hayden et al,WO/2009/135322) associated with the HTT gene were mapped to the twosequences and have been demarcated in FIG. 1 by their reference SNP IDnumber from the Entrez SNP database at the National Center forBiotechnology Information (NCBI,http://www.ncbi.nlm.nih.gov/sites/entrez?db=snp), incorporated herein byreference. Table 2 furnishes further details on each SNP. The ‘ReferenceSNP ID number’ or ‘RS number’ is the number designated to each SNP fromthe Entrez SNP database at NCBI, incorporated herein by reference. ‘SNPposition’ refers to the nucleotide position of the SNP on SEQ ID NO: 1.‘Polymorphism’ indicates the nucleotide variants at that SNP position.‘Major allele’ indicates the nucleotide associated with the majorallele, or the nucleotide present in a statistically significantproportion of individuals in the human population. ‘Minor allele’indicates the nucleotide associated with the minor allele, or thenucleotide present in a relatively small proportion of individuals inthe human population.

TABLE 2 Single Nuclear Polymorphisms (SNPs) and their positions on SEQID NO: 1 SNP Major Minor RS No. position Polymorphism allele allelers2857936 1963 C/T C T rs12506200 3707 A/G G A rs762855 14449 A/G G Ars3856973 19826 G/A G A rs2285086 28912 G/A A G rs7659144 37974 C/G C Grs16843804 44043 C/T C T rs2024115 44221 G/A A G rs10015979 49095 A/G AG rs7691627 51063 A/G G A rs2798235 54485 G/A G A rs4690072 62160 G/T TG rs6446723 66466 C/T T C rs363081 73280 G/A G A rs363080 73564 T/C C Trs363075 77327 G/A G A rs363064 81063 T/C C T rs3025849 83420 A/G A Grs6855981 87929 A/G G A rs363102 88669 G/A A G rs11731237 91466 C/T C Trs4690073 99803 A/G G A rs363144 100948 T/G T G rs3025838 101099 C/T C Trs34315806 101687 A/G G A rs363099 101709 T/C C T rs363096 119674 T/C TC rs2298967 125400 C/T T C rs2298969 125897 A/G G A rs6844859 130139 C/TT C rs363092 135682 C/A C A rs7685686 146795 A/G A G rs363088 149983 A/TA T rs362331 155488 C/T T C rs916171 156468 G/C C G rs362322 161018 A/GA G rs362275 164255 T/C C T rs362273 167080 A/G A G rs2276881 171314 G/AG A rs3121419 171910 T/C C T rs362272 174633 G/A G A rs362271 175171 G/AG A rs3775061 178407 C/T C T rs362310 179429 A/G G A rs362307 181498 T/CC T rs362306 181753 G/A G A rs362303 181960 T/C C T rs362296 186660 C/AC A rs1006798 198026 A/G A G

Example 2 Design of Antisense Oligonucleotides Targeting Huntingtin GeneSNPs and Inhibition of HTT mRNA in Coriell Fibroblast Cell Lines(GM04281, GM02171, and GM02173B)

Antisense oligonucleotides targeting nucleotides overlapping SNPpositions presented in Table 1 were designed and tested for potency inthree huntingtin patient-derived Coriell fibroblast cell lines, GM04281,GM02171, and GM02173B (from the Coriell Institute for Medical Research).Cultured GM04281 cells or GM02171 cells or GM02173B cells at a densityof 20,000 cells per well were transfected using electroporation with10,000 nM antisense oligonucleotide. After a treatment period ofapproximately 24 hours, RNA was isolated from the cells and HTT mRNAlevels were measured by quantitative real time PCR using primer probeset RTS2617 (forward sequence CTCCGTCCGGTAGACATGCT, designated herein asSEQ ID NO: 3; reverse sequence GGAAATCAGAACCCTCAAAATGG, designatedherein as SEQ ID NO: 4; probe sequence TGAGCACTGTTCAACTGTGGATATCGGGAX,designated herein as SEQ ID NO: 5). HTT mRNA levels were adjustedaccording to total RNA content, as measured by RIBOGREEN. Results arepresented as percent inhibition of HTT mRNA, relative to untreatedcontrol cells.

ISIS 387916 (TCTCTATTGCACATTCCAAG, 5-10-5 MOE (SEQ ID NO: 6)) and ISIS388816 (GCCGTAGCCTGGGACCCGCC, 5-10-5 MOE (SEQ ID NO: 7)) were includedin each study as benchmark oligonucleotides against which the potency ofthe antisense oligonucleotides targeting nucleotides overlapping eachSNP position could be compared.

The chimeric antisense oligonucleotides in Tables 3 and 4 were designedas 5-9-5 MOE gapmers. The gapmers are 19 nucleotides in length, whereinthe central gap segment is comprised of nine 2′-deoxynucleotides and isflanked on both sides (in the 5′ and 3′ directions) by wings comprisingfive nucleotides each. Each nucleotide in the 5′ wing segment and eachnucleotide in the 3′ wing segment has a 2′-MOE modification. Theinternucleoside linkages throughout each gapmer are phosphorothioate(P═S) linkages. All cytosine nucleobases throughout each gapmer are5-methylcytosines.

The oligonucleotides are further described in Table 3. The percentinhibition of HTT mRNA by the antisense oligonucleotides in each cellline is shown in Table 4. ‘Target allele’ indicates whether the gapmeris targeted to the major or the minor allele at the SNP position. Thenumber in parentheses indicates the nucleotide position in the gapmeropposite to the SNP position, starting from the 5′-terminus of theoligonucleotide. ‘Start site’ indicates the 5′-most nucleotide to whichthe gapmer is targeted. “Stop site” indicates the 3′-most nucleotide towhich the gapmer is targeted. Each gapmer listed in Tables 3 and 4 istargeted to human HTT pre-mRNA, which is SEQ ID NO: 1.

TABLE 3 Chimeric oligonucleotides targeting SNP positions on the HTTgene SEQ ISIS SNP RS Target Start Stop ID No No. allele Sequence SiteSite NO 387916 n/a n/a TCTCTATTGCACATTCCAAG 145466 145485 6 388816 n/an/a GCCGTAGCCTGGGACCCGCC 16501 16520 7 435330 rs3856973 Major (8)TAACACTCGATTAACCCTG 19815 19833 8 435348 rs3856973 Minor (8)TAACACTTGATTAACCCTG 19815 19833 9 435294 rs3856973 Major (10)GTTAACACTCGATTAACCC 19817 19835 10 435312 rs3856973 Minor (10)GTTAACACTTGATTAACCC 19817 19835 11 435864 rs2285086 Major (10)GCTAGTTCATCCCAGTGAG 28903 28921 12 435889 rs2285086 Minor (10)GCTAGTTCACCCCAGTGAG 28903 28921 13 435878 rs7659144 Major (10)TGGAAATGGGTTTTTCCAC 37965 37983 14 435903 rs7659144 Minor (10)TGGAAATGGCTTTTTCCAC 37965 37983 15 435863 rs16843804 Major (10)TTTAACCGTGGCATGGGCA 44034 44052 16 435888 rs16843804 Minor (10)TTTAACCGTAGCATGGGCA 44034 44052 17 435331 rs2024115 Major (8)TTCAAGCTAGTAACGATGC 44210 44228 18 435349 rs2024115 Minor (8)TTCAAGCCAGTAACGATGC 44210 44228 19 435295 rs2024115 Major (10)ACTTCAAGCTAGTAACGAT 44212 44230 20 435313 rs2024115 Minor (10)ACTTCAAGCCAGTAACGAT 44212 44230 21 435862 rs10015979 Major (10)GCAGCTAGGTTAAAGAGTC 49086 49104 22 435887 rs10015979 Minor (10)GCAGCTAGGCTAAAGAGTC 49086 49104 23 435880 rs7691627 Major (10)AATAAGAAACACAATCAAA 51054 51072 24 435905 rs7691627 Minor (10)AATAAGAAATACAATCAAA 51054 51072 25 435885 rs2798235 Major (10)CAGAGGAGGCATACTGTAT 54476 54494 26 435910 rs2798235 Minor (10)CAGAGGAGGTATACTGTAT 54476 54494 27 435874 rs4690072 Major (10)CACAGTGCTACCCAACCTT 62151 62169 28 435899 rs4690072 Minor (10)CACAGTGCTCCCCAACCTT 62151 62169 29 435875 rs6446723 Major (10)TAATTTTCTAGACTTTATG 66457 66475 30 435900 rs6446723 Minor (10)TAATTTTCTGGACTTTATG 66457 66475 31 435332 rs363081 Major (8)GCTACAACGCAGGTCAAAT 73269 73287 32 435350 rs363081 Minor (8)GCTACAATGCAGGTCAAAT 73269 73287 33 435296 rs363081 Major (10)GAGCTACAACGCAGGTCAA 73271 73289 34 435314 rs363081 Minor (10)GAGCTACAATGCAGGTCAA 73271 73289 35 435886 rs363080 Major (10)AGAGAGAACGAGAAGGCTC 73555 73573 36 435911 rs363080 Minor (10)AGAGAGAACAAGAAGGCTC 73555 73573 37 435914 rs363075 Major (6)AGCCCCTCTGTGTAAGTTT 77314 77332 38 435926 rs363075 Minor (6)AGCCCTTCTGTGTAAGTTT 77314 77332 39 435916 rs363075 Major (7)GAGCCCCTCTGTGTAAGTT 77315 77333 40 435928 rs363075 Minor (7)GAGCCCTTCTGTGTAAGTT 77315 77333 41 435333 rs363075 Major (8)TGAGCCCCTCTGTGTAAGT 77316 77334 42 435351 rs363075 Minor (8)TGAGCCCTTCTGTGTAAGT 77316 77334 43 435918 rs363075 Major (9)ATGAGCCCCTCTGTGTAAG 77317 77335 44 435930 rs363075 Minor (9)ATGAGCCCTTCTGTGTAAG 77317 77335 45 435297 rs363075 Major (10)GATGAGCCCCTCTGTGTAA 77318 77336 46 435315 rs363075 Minor (10)GATGAGCCCTTCTGTGTAA 77318 77336 47 435920 rs363075 Major (11)TGATGAGCCCCTCTGTGTA 77319 77337 48 435932 rs363075 Minor (11)TGATGAGCCCTTCTGTGTA 77319 77337 49 435366 rs363075 Major (12)ATGATGAGCCCCTCTGTGT 77320 77338 50 435924 rs363075 Minor (12)ATGATGAGCCCTTCTGTGT 77320 77338 51 435922 rs363075 Major (14)TAATGATGAGCCCCTCTGT 77322 77340 52 435934 rs363075 Minor (14)TAATGATGAGCCCTTCTGT 77322 77340 53 435334 rs363064 Major (8)AGAATACGGGTAACATTTT 81052 81070 54 435352 rs363064 Minor (8)AGAATACAGGTAACATTTT 81052 81070 55 435298 rs363064 Major (10)GGAGAATACGGGTAACATT 81054 81072 56 435316 rs363064 Minor (10)GGAGAATACAGGTAACATT 81054 81072 57 435335 rs3025849 Major (8)TTAGTAATCAATTTTAATG 83409 83427 58 435353 rs3025849 Minor (8)TTAGTAACCAATTTTAATG 83409 83427 59 435299 rs3025849 Major (10)AGTTAGTAATCAATTTTAA 83411 83429 60 435317 rs3025849 Minor (10)AGTTAGTAACCAATTTTAA 83411 83429 61 435877 rs6855981 Major (10)GAAGGAATGCTTTTACTAG 87920 87938 62 435902 rs6855981 Minor (10)GAAGGAATGTTTTTACTAG 87920 87938 63 435336 rs363102 Major (8)CTAAAACTAACTTGAGAAT 88658 88676 64 435354 rs363102 Minor (8)CTAAAACCAACTTGAGAAT 88658 88676 65 435300 rs363102 Major (10)ATCTAAAACTAACTTGAGA 88660 88678 66 435318 rs363102 Minor (10)ATCTAAAACCAACTTGAGA 88660 88678 67 435884 rs11731237 Major (10)GGTGGGCAGGAAGGACTGA 91457 91475 68 435909 rs11731237 Minor (10)GGTGGGCAGAAAGGACTGA 91457 91475 69 435337 rs4690073 Major (8)CCTAAATCAATCTACAAGT 99792 99810 70 435355 rs4690073 Minor (8)CCTAAATTAATCTACAAGT 99792 99810 71 435301 rs4690073 Major (10)TCCCTAAATCAATCTACAA 99794 99812 72 435319 rs4690073 Minor (10)TCCCTAAATTAATCTACAA 99794 99812 73 435883 rs363144 Major (10)GAAAATGTGAGTGGATCTA 100939 100957 74 435908 rs363144 Minor (10)GAAAATGTGCGTGGATCTA 100939 100957 75 435338 rs3025838 Major (8)GTAAGGCGAGACTGACTAG 101088 101106 76 435356 rs3025838 Minor (8)GTAAGGCAAGACTGACTAG 101088 101106 77 435302 rs3025838 Major (10)AGGTAAGGCGAGACTGACT 101090 101108 78 435320 rs3025838 Minor (10)AGGTAAGGCAAGACTGACT 101090 101108 79 435339 rs363099 Major (8)CTGAGCGGAGAAACCCTCC 101698 101716 80 435357 rs363099 Minor (8)CTGAGCGAAGAAACCCTCC 101698 101716 81 435303 rs363099 Major (10)GGCTGAGCGGAGAAACCCT 101700 101718 82 435321 rs363099 Minor (10)GGCTGAGCGAAGAAACCCT 101700 101718 83 435367 rs363099 Major (12)AAGGCTGAGCGGAGAAACC 101702 101720 84 435340 rs363096 Major (8)TTCCCTAAAAACAAAAACA 119663 119681 85 435358 rs363096 Minor (8)TTCCCTAGAAACAAAAACA 119663 119681 86 435304 rs363096 Major (10)GATTCCCTAAAAACAAAAA 119665 119683 87 435322 rs363096 Minor (10)GATTCCCTAGAAACAAAAA 119665 119683 88 435341 rs2298967 Major (8)CTTTTCTATTGTCTGTCCC 125389 125407 89 435359 rs2298967 Minor (8)CTTTTCTGTTGTCTGTCCC 125389 125407 90 435305 rs2298967 Major (10)TGCTTTTCTATTGTCTGTC 125391 125409 91 435323 rs2298967 Minor (10)TGCTTTTCTGTTGTCTGTC 125391 125409 92 435865 rs2298969 Major (10)AAGGGATGCCGACTTGGGC 125888 125906 93 435890 rs2298969 Minor (10)AAGGGATGCTGACTTGGGC 125888 125906 94 435876 rs6844859 Major (10)ACCTTCCTCACTGAGGATG 130130 130148 95 435901 rs6844859 Minor (10)ACCTTCCTCGCTGAGGATG 130130 130148 96 435872 rs363092 Major (10)CAAACCACTGTGGGATGAA 135673 135691 97 435897 rs363092 Minor (10)CAAACCACTTTGGGATGAA 135673 135691 98 435879 rs7685686 Major (10)AATAAATTGTCATCACCAG 146786 146804 99 435904 rs7685686 Minor (10)AATAAATTGCCATCACCAG 146786 146804 100 435871 rs363088 Major (10)TCACAGCTATCTTCTCATC 149974 149992 101 435896 rs363088 Minor (10)TCACAGCTAACTTCTCATC 149974 149992 102 435870 rs362331 Major (10)GCACACAGTAGATGAGGGA 155479 155497 103 435895 rs362331 Minor (10)GCACACAGTGGATGAGGGA 155479 155497 104 435881 rs916171 Major (10)CAGAACAAAGAGAAGAATT 156459 156477 105 435906 rs916171 Minor (10)CAGAACAAACAGAAGAATT 156459 156477 106 435342 rs362322 Major (8)GCTTACATGCCTTCAGTGA 161007 161025 107 435360 rs362322 Minor (8)GCTTACACGCCTTCAGTGA 161007 161025 108 435306 rs362322 Major (10)CAGCTTACATGCCTTCAGT 161009 161027 109 435324 rs362322 Minor (10)CAGCTTACACGCCTTCAGT 161009 161027 110 435868 rs362275 Major (10)AAGAAGCCTGATAAAATCT 164246 164264 111 435893 rs362275 Minor (10)AAGAAGCCTAATAAAATCT 164246 164264 112 435343 rs2276881 Major (8)CATACATCAGCTCAAACTG 171303 171321 113 435361 rs2276881 Minor (8)CATACATTAGCTCAAACTG 171303 171321 114 435307 rs2276881 Major (10)CACATACATCAGCTCAAAC 171305 171323 115 435325 rs2276881 Minor (10)CACATACATTAGCTCAAAC 171305 171323 116 435368 rs2276881 Major (12)GTCACATACATCAGCTCAA 171307 171325 117 435866 rs3121419 Major (10)GAGACTATAGCACCCAGAT 171901 171919 118 435891 rs3121419 Minor (10)GAGACTATAACACCCAGAT 171901 171919 119 435344 rs362272 Major (8)TAGAGGACGCCGTGCAGGG 174622 174640 120 435362 rs362272 Minor (8)TAGAGGATGCCGTGCAGGG 174622 174640 121 435308 rs362272 Major (10)CATAGAGGACGCCGTGCAG 174624 174642 122 435326 rs362272 Minor (10)CATAGAGGATGCCGTGCAG 174624 174642 123 435369 rs362272 Major (12)CACATAGAGGACGCCGTGC 174626 174644 124 435867 rs362271 Major (10)ACGTGTGTACAGAACCTGC 175162 175180 125 435892 rs362271 Minor (10)ACGTGTGTATAGAACCTGC 175162 175180 126 435873 rs3775061 Major (10)TGTTCAGAATGCCTCATCT 178398 178416 127 435898 rs3775061 Minor (10)TGTTCAGAACGCCTCATCT 178398 178416 128 435345 rs362310 Major (8)AAACGGCGCAGCGGGAAGG 179418 179436 129 435363 rs362310 Minor (8)AAACGGCACAGCGGGAAGG 179418 179436 130 435309 rs362310 Major (10)AGAAACGGCGCAGCGGGAA 179420 179438 131 435327 rs362310 Minor (10)AGAAACGGCACAGCGGGAA 179420 179438 132 435915 rs362307 Major (6)AGGGCGCAGACTTCCAAAG 181485 181503 133 435927 rs362307 Minor (6)AGGGCACAGACTTCCAAAG 181485 181503 134 435917 rs362307 Major (7)AAGGGCGCAGACTTCCAAA 181486 181504 135 435929 rs362307 Minor (7)AAGGGCACAGACTTCCAAA 181486 181504 136 435346 rs362307 Major (8)CAAGGGCGCAGACTTCCAA 181487 181505 137 435364 rs362307 Minor (8)CAAGGGCACAGACTTCCAA 181487 181505 138 435919 rs362307 Major (9)ACAAGGGCGCAGACTTCCA 181488 181506 139 435931 rs362307 Minor (9)ACAAGGGCACAGACTTCCA 181488 181506 140 435310 rs362307 Major (10)CACAAGGGCGCAGACTTCC 181489 181507 141 435328 rs362307 Minor (10)CACAAGGGCACAGACTTCC 181489 181507 142 435921 rs362307 Major (11)GCACAAGGGCGCAGACTTC 181490 181508 143 435933 rs362307 Minor (11)GCACAAGGGCACAGACTTC 181490 181508 144 435370 rs362307 Major (12)GGCACAAGGGCGCAGACTT 181491 181509 145 435925 rs362307 Minor (12)GGCACAAGGGCACAGACTT 181491 181509 146 435923 rs362307 Major (14)AGGGCACAAGGGCGCAGAC 181493 181511 147 435935 rs362307 Minor (14)AGGGCACAAGGGCACAGAC 181493 181511 148 435869 rs362306 Major (10)GAGCAGCTGCAACCTGGCA 181744 181762 149 435894 rs362306 Minor (10)GAGCAGCTGTAACCTGGCA 181744 181762 150 435347 rs362303 Major (8)TGGTGCCGGGTGTCTAGCA 181949 181967 151 435365 rs362303 Minor (8)TGGTGCCAGGTGTCTAGCA 181949 181967 152 435311 rs362303 Major (10)AATGGTGCCGGGTGTCTAG 181951 181969 153 435329 rs362303 Minor (10)AATGGTGCCAGGTGTCTAG 181951 181969 154 435882 rs362296 Major (10)GGGGACAGGGTGTGCTCTC 186651 186669 155 435907 rs362296 Minor (10)GGGGACAGGTTGTGCTCTC 186651 186669 156

TABLE 4 Comparison of inhibition of HTT mRNA levels by ISIS 387916 andISIS 388816 with that by chimeric oligonucleotides targeting SNPpositions on the HTT gene (SEQ ID NO: 1) SEQ SNP RS Target % inhibitionID ISIS No No. allele GM04281 GM02171 GM02173B NO 387916 n/a n/a 96 9698 6 388816 n/a n/a 76 88 85 7 435330 rs3856973 Major (8) 64 51 36 8435348 rs3856973 Minor (8) 50 88 80 9 435294 rs3856973 Major (10) 54 4654 10 435312 rs3856973 Minor (10) 20 82 58 11 435864 rs2285086 Major(10) 54 28 26 12 435889 rs2285086 Minor (10) 17 43 41 13 435878rs7659144 Major (10) 43 32 39 14 435903 rs7659144 Minor (10) 16 37 29 15435863 rs16843804 Major (10) 63 78 81 16 435888 rs16843804 Minor (10) 5875 77 17 435331 rs2024115 Major (8) 56 27 56 18 435349 rs2024115 Minor(8) 26 91 66 19 435295 rs2024115 Major (10) 53 57 62 20 435313 rs2024115Minor (10) 25 87 53 21 435862 rs10015979 Major (10) 8 51 40 22 435887rs10015979 Minor (10) 40 22 28 23 435880 rs7691627 Major (10) 43 17 2124 435905 rs7691627 Minor (10) 13 27 15 25 435885 rs2798235 Major (10)38 39 30 26 435910 rs2798235 Minor (10) 17 30 16 27 435874 rs4690072Major (10) 61 34 48 28 435899 rs4690072 Minor (10) 50 41 45 29 435875rs6446723 Major (10) 28 13 35 30 435900 rs6446723 Minor (10) 24 56 37 31435332 rs363081 Major (8) 76 95 88 32 435350 rs363081 Minor (8) 27 61 4333 435296 rs363081 Major (10) 59 77 66 34 435314 rs363081 Minor (10) 3866 40 35 435886 rs363080 Major (10) 74 72 79 36 435911 rs363080 Minor(10) 57 58 54 37 435914 rs363075 Major (6) 95 92 95 38 435926 rs363075Minor (6) 88 81 79 39 435916 rs363075 Major (7) 90 92 94 40 435928rs363075 Minor (7) 83 79 85 41 435333 rs363075 Major (8) 86 97 91 42435351 rs363075 Minor (8) 59 80 58 43 435918 rs363075 Major (9) 83 90 9144 435930 rs363075 Minor (9) 29 49 49 45 435297 rs363075 Major (10) 7484 83 46 435315 rs363075 Minor (10) 47 63 45 47 435920 rs363075 Major(11) 78 66 83 48 435932 rs363075 Minor (11) 39 20 19 49 435366 rs363075Major (12) 80 91 85 50 435924 rs363075 Minor (12) 37 49 58 51 435922rs363075 Major (14) 80 90 91 52 435934 rs363075 Minor (14) 63 70 80 53435334 rs363064 Major (8) 50 59 44 54 435352 rs363064 Minor (8) 12 37 4855 435298 rs363064 Major (10) 81 92 87 56 435316 rs363064 Minor (10) 6990 80 57 435335 rs3025849 Major (8) 0 40 37 58 435353 rs3025849 Minor(8) 0 29 18 59 435299 rs3025849 Major (10) 0 34 67 60 435317 rs3025849Minor (10) 0 38 34 61 435877 rs6855981 Major (10) 31 59 58 62 435902rs6855981 Minor (10) 0 43 27 63 435336 rs363102 Major (8) 0 21 19 64435354 rs363102 Minor (8) 0 36 33 65 435300 rs363102 Major (10) 0 34 2466 435318 rs363102 Minor (10) 0 30 20 67 435884 rs11731237 Major (10) 746 51 68 435909 rs11731237 Minor (10) 30 47 41 69 435337 rs4690073 Major(8) 12 0 12 70 435355 rs4690073 Minor (8) 0 26 33 71 435301 rs4690073Major (10) 23 0 10 72 435319 rs4690073 Minor (10) 0 45 53 73 435883rs363144 Major (10) 24 23 39 74 435908 rs363144 Minor (10) 27 20 22 75435338 rs3025838 Major (8) 31 46 69 76 435356 rs3025838 Minor (8) 3 2517 77 435302 rs3025838 Major (10) 39 73 67 78 435320 rs3025838 Minor(10) 21 49 32 79 435339 rs363099 Major (8) 84 87 76 80 435357 rs363099Minor (8) 71 91 90 81 435303 rs363099 Major (10) 83 92 85 82 435321rs363099 Minor (10) 84 95 89 83 435367 rs363099 Major (12) 76 82 72 84435340 rs363096 Major (8) 0 47 52 85 435358 rs363096 Minor (8) 0 25 3586 435304 rs363096 Major (10) 5 33 36 87 435322 rs363096 Minor (10) 2 3032 88 435341 rs2298967 Major (8) 54 72 56 89 435359 rs2298967 Minor (8)25 59 63 90 435305 rs2298967 Major (10) 66 80 78 91 435323 rs2298967Minor (10) 36 79 66 92 435865 rs2298969 Major (10) 53 72 79 93 435890rs2298969 Minor (10) 65 46 54 94 435876 rs6844859 Major (10) 70 67 77 95435901 rs6844859 Minor (10) 39 83 80 96 435872 rs363092 Major (10) 46 4154 97 435897 rs363092 Minor (10) 37 69 57 98 435879 rs7685686 Major (10)83 31 70 99 435904 rs7685686 Minor (10) 30 92 72 100 435871 rs363088Major (10) 70 55 70 101 435896 rs363088 Minor (10) 66 74 80 102 435870rs362331 Major (10) 88 74 88 103 435895 rs362331 Minor (10) 78 92 86 104435881 rs916171 Major (10) 0 57 51 105 435906 rs916171 Minor (10) 14 2617 106 435342 rs362322 Major (8) 47 74 67 107 435360 rs362322 Minor (8)17 58 52 108 435306 rs362322 Major (10) 50 77 65 109 435324 rs362322Minor (10) 42 61 64 110 435868 rs362275 Major (10) 54 35 43 111 435893rs362275 Minor (10) 3 27 33 112 435343 rs2276881 Major (8) 59 76 65 113435361 rs2276881 Minor (8) 58 44 20 114 435307 rs2276881 Major (10) 6982 81 115 435325 rs2276881 Minor (10) 17 47 43 116 435368 rs2276881Major (12) 84 96 92 117 435866 rs3121419 Major (10) 67 61 64 118 435891rs3121419 Minor (10) 53 76 73 119 435344 rs362272 Major (8) 35 46 36 120435362 rs362272 Minor (8) 34 68 57 121 435308 rs362272 Major (10) 26 3035 122 435326 rs362272 Minor (10) 29 50 39 123 435369 rs362272 Major(12) 66 74 65 124 435867 rs362271 Major (10) 73 74 75 125 435892rs362271 Minor (10) 52 74 79 126 435873 rs3775061 Major (10) 40 32 47127 435898 rs3775061 Minor (10) 13 20 24 128 435345 rs362310 Major (8)38 55 52 129 435363 rs362310 Minor (8) 45 67 60 130 435309 rs362310Major (10) 33 44 56 131 435327 rs362310 Minor (10) 33 71 61 132 435915rs362307 Major (6) 61 54 58 133 435927 rs362307 Minor (6) 31 35 44 134435917 rs362307 Major (7) 67 76 66 135 435929 rs362307 Minor (7) 33 3455 136 435346 rs362307 Major (8) 67 89 66 137 435364 rs362307 Minor (8)46 72 66 138 435919 rs362307 Major (9) 84 79 70 139 435931 rs362307Minor (9) 74 74 86 140 435310 rs362307 Major (10) 74 81 71 141 435328rs362307 Minor (10) 47 69 75 142 435921 rs362307 Major (11) 74 77 69 143435933 rs362307 Minor (11) 38 47 74 144 435370 rs362307 Major (12) 64 7438 145 435925 rs362307 Minor (12) 60 66 80 146 435923 rs362307 Major(14) 73 66 71 147 435935 rs362307 Minor (14) 68 75 87 148 435869rs362306 Major (10) 82 77 81 149 435894 rs362306 Minor (10) 28 79 72 150435347 rs362303 Major (8) 68 74 71 151 435365 rs362303 Minor (8) 69 8376 152 435311 rs362303 Major (10) 46 56 72 153 435329 rs362303 Minor(10) 49 62 39 154 435882 rs362296 Major (10) 29 48 56 155 435907rs362296 Minor (10) 42 56 52 156

Example 3 Dose-Dependent Antisense Inhibition of Human Huntingtin mRNALevels in Coriell Fibroblast Cell Lines

Gapmers from the study described in Example 2 were selected and testedat various doses in GM04281, GM02171, and GM02173B cell lines. Each cellline was plated at a density of 25,000 cells per well and transfectedusing electroporation with 750 nM, 1,500 nM, 3,000 nM, 6,000 nM, and12,000 nM concentrations of antisense oligonucleotide, as specified inTable 5, 6, and 7. After a treatment period of approximately 16 hours,RNA was isolated from the cells and HTT mRNA levels were measured byquantitative real-time PCR. Human HTT primer probe set RTS2617 was usedto measure mRNA levels. HTT mRNA levels were adjusted according to totalRNA content, as measured by RIBOGREEN. Results are presented as percentinhibition of HTT mRNA, relative to untreated control cells. IC₅₀ valuesare also provided in Tables 5, 6, and 7.

TABLE 5 Dose-dependent antisense inhibition of human HTT in GM04281cells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 51 81 80 91 97 0.6 435330 24 49 50 73 85 2.5 435331 23 38 64 7274 2.4 435868 3 17 7 29 63 6.7 435870 53 73 77 86 93 0.6 435871 28 51 5278 89 1.7 435874 14 21 28 64 82 3.3 435879 42 57 57 81 91 1.1 435890 4856 62 76 91 0.9 435929 10 0 5 12 48 13.8 435931 20 17 53 62 81 2.9435933 0 7 24 43 49 10.7 435935 0 38 38 62 29 4.2

TABLE 6 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 57 73 81 93 98 0.4 435330 27 37 0 44 63 4.4 435331 35 34 19 41 633.5 435868 21 21 39 24 12 >12.0 435870 50 53 57 70 79 0.9 435871 32 4645 58 62 3.9 435874 1 0 4 11 6 >12.0 435879 32 14 17 45 38 >12.0 43589034 33 40 51 62 5.4 435929 25 22 31 5 29 >12.0 435931 15 28 27 60 79 3.7435933 13 36 21 43 48 12.2 435935 25 42 27 61 68 3.2

TABLE 7 Dose-dependent antisense inhibition of human HTT in GM02173Bcells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 43 67 80 86 97 1.1 435330 22 21 0 52 62 5.3 435331 19 17 32 50 559.4 435868 17 25 41 13 26 >12.0 435870 24 57 70 78 75 1.8 435871 8 30 4250 48 5.0 435874 31 35 28 35 42 >12.0 435879 39 44 42 60 64 2.5 43589038 36 50 65 73 3.1 435929 19 17 19 42 35 7.7 435931 40 19 31 48 71 5.8435933 35 24 47 52 59 4.4 435935 25 23 40 73 77 3.7

Example 4 Dose-Dependent Antisense Inhibition of Human Huntingtin mRNALevels in Coriell Fibroblast Cell Lines

Gapmers from the study described in Example 2 were selected and testedat various doses in GM04281, GM02171, and GM02173B cell lines. Each cellline was plated at a density of 25,000 cells per well and transfectedusing electroporation with 750 nM, 1,500 nM, 3,000 nM, 6,000 nM, and12,000 nM concentrations of antisense oligonucleotide, as specified inTable 8, 9, and 10. After a treatment period of approximately 16 hours,RNA was isolated from the cells and HTT mRNA levels were measured byquantitative real-time PCR. Human HTT primer probe set RTS2617 was usedto measure mRNA levels. HTT mRNA levels were adjusted according to totalRNA content, as measured by RIBOGREEN. Results are presented as percentinhibition of HTT mRNA relative to untreated control cells. IC₅₀ valuesare also provided in Tables 8, 9, and 10.

TABLE 8 Dose-dependent antisense inhibition of human HTT in GM04281cells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 61 78 90 94 97 <0.8 435303 33 39 69 79 91 1.5 435328 0 12 16 5175 5.3 435331 27 48 48 70 82 2.1 435339 46 37 61 73 89 2.3 435869 17 3544 66 80 3.3 435870 44 60 64 84 84 1.1 435871 41 50 71 78 87 1.2 43587424 36 35 65 73 3.1 435879 46 52 78 81 92 0.9 435890 41 53 63 80 86 1.3435925 0 14 39 60 87 4.2 435926 20 28 67 81 89 2.0 435928 32 49 73 86 861.8 435931 22 24 40 59 90 3.8

TABLE 9 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 50 64 90 95 96 0.7 435303 14 32 68 79 85 2.8 435328 0 12 20 38 5510.3 435331 0 13 5 30 36 >12.0 435339 30 40 58 63 49 2.5 435869 13 25 3147 87 4.0 435870 18 31 44 66 74 3.5 435871 1 20 29 49 64 6.5 435874 3 612 17 31 >12.0 435879 0 2 12 35 44 >12.0 435890 15 16 30 48 72 5.8435925 0 0 22 48 29 6.3 435926 25 28 58 74 85 2.3 435928 18 53 61 86 832.5 435931 0 4 25 46 68 6.7

TABLE 10 Dose-dependent antisense inhibition of human HTT in GM02173Bcells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 27 65 84 81 96 1.9 435303 23 48 52 76 76 2.9 435328 8 14 19 34 5015.7 435331 10 17 16 27 32 >12.0 435339 28 26 38 67 82 3.8 435869 12 2437 45 79 4.2 435870 20 26 58 53 78 2.7 435871 15 16 32 45 71 6.0 43587413 8 28 36 31 >12.0 435879 22 20 36 53 60 6.0 435890 21 28 34 54 71 4.3435925 2 10 28 43 78 5.9 435926 7 25 37 73 79 3.5 435928 15 39 60 73 872.5 435931 13 13 32 61 62 6.7

Example 5 Antisense Inhibition of Human HTT in GM04281 Cells

Additional antisense oligonucleotides were designed based on the gapmersselected from studies described in Example 4. These oligonucleotideswere designed by creating gapmers shifted slightly upstream anddownstream (i.e. “microwalk”) of the original gapmers from Tables 8, 9,and 10. Antisense oligonucleotides were also created with uniform MOE,as well as with various motifs, 2-9-6 MOE, 3-9-3 MOE, 3-9-4 MOE, 3-9-5MOE, 4-10-5 MOE, 4-11-4 MOE, 4-7-4 MOE, 4-9-4 MOE, 4-9-5 MOE, 5-10-4MOE, 5-7-5 MOE, 5-8-6 MOE, 5-9-3 MOE, 5-9-5 MOE, 6-7-6 MOE, 6-9-2 MOE,and 6-8-5 MOE.

In addition, antisense oligonucleotides were designed targeting SNP RSNos. rs2857936, rs12506200, rs762855, and rs1006798 (refer to Table 2).The oligonucleotides were designed targeting either the major allele orthe minor allele, and with the SNP position opposite either position 8or position 10 of the gapmer.

These gapmers were tested in vitro. Cultured GM04281 cells at a densityof 25,000 cells per well were transfected using electroporation with10,000 nM antisense oligonucleotide. After a treatment period ofapproximately 24 hours, RNA was isolated from the cells and HTT mRNAlevels were measured by quantitative real-time PCR. HTT mRNA levels wereadjusted according to total RNA content, as measured by RIBOGREEN.Results are presented in Tables 11-19 as percent inhibition of HTT mRNA,relative to untreated control cells.

The gapmers, ISIS 435869, ISIS 435870, ISIS 435874, ISIS 435879, andISIS 435890, from which some of the newly designed gapmers were derivedare marked with an asterisk (*) in the table. ISIS 387916 was includedin the study as a benchmark oligonucleotide against which the potency ofthe antisense oligonucleotides targeting nucleotides overlapping eachSNP position could be compared.

The uniform MOE oligonucleotides are 15 nucleotides in length.

The 2-9-6 gapmers are 17 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 2 nucleotides and on the 3′ directionby a wing comprising 6 nucleotides.

The 3-9-3 gapmers are 15 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 3 nucleotides each.

The 3-9-4 gapmers are 16 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 3 nucleotides and on the 3′ directionby a wing comprising 4 nucleotides.

The 3-9-5 gapmers are 17 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 3 nucleotides and on the 3′ directionby a wing comprising 5 nucleotides.

The 4-10-5 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of ten 2′-deoxynucleotides and is flanked on the 5′direction by a wing comprising 4 nucleotides and on the 3′ direction bya wing comprising 5 nucleotides.

The 4-11-4 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of eleven 2′-deoxynucleotides and is flanked onboth 5′ and 3′ directions by wings comprising 4 nucleotides each.

The 4-7-4 gapmers are 15 nucleotides in length, wherein the central gapsegment is comprised of seven 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 4 nucleotides each.

The 4-9-4 gapmers are 17 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 4 nucleotides each.

The 4-9-5 gapmers are 18 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 4 nucleotides and on the 3′ directionby a wing comprising 5 nucleotides.

The 5-10-4 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of ten 2′-deoxynucleotides and is flanked on the 5′direction by a wing comprising 5 nucleotides and on the 3′ direction bya wing comprising 4 nucleotides.

The 5-7-5 gapmers are 17 nucleotides in length, wherein the central gapsegment is comprised of seven 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 5 nucleotides each.

The 5-8-6 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of eight 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 5 nucleotides and on the 3′ directionby a wing comprising 6 nucleotides.

The 5-9-3 gapmers are 17 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 5 nucleotides and on the 3′ directionby a wing comprising 3 nucleotides.

The 5-9-5 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 5 nucleotides each.

The 6-7-6 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of seven 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 6 nucleotides each.

The 6-9-2 gapmers are 17 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 6 nucleotides and on the 3′ directionby a wing comprising 2 nucleotides.

The 6-8-5 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of eight 2′-deoxynucleotides and is flanked on the5′ direction by a wing comprising 6 nucleotides and on the 3′ directionby a wing comprising 5 nucleotides.

For each of the motifs, each nucleotide in the 5′ wing segment and eachnucleotide in the 3′ wing segment has a 2′-MOE modification. Theinternucleoside linkages throughout each gapmer are phosphorothioate(P═S) linkages. All cytosine nucleobases throughout each gapmer are5-methylcytosines.

The oligonucleotides are organized in tables according to the SNP theytarget. “Start site” indicates the 5′-most nucleotide to which thegapmer is targeted. “Stop site” indicates the 3′-most nucleotide towhich the gapmer is targeted. ‘Target allele’ indicates whether thegapmer is targeted to the major or the minor allele. The number inparentheses indicates the position on the oligonucleotide opposite tothe SNP position.

TABLE 11 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs2857936(nucleobases 1952 to 1972 of SEQ ID NO: 1) SEQ Start Stop Target % IDSite Site allele ISIS No. Sequence Motif inhibition NO 145466 145485 n/a387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 1952 1970 Minor (8) 459908GCTTTTCATTGAAAAGAAA 5-9-5 26 157 1952 1970 Major (8) 459916GCTTTTCGTTGAAAAGAAA 5-9-5 8 158 1954 1972 Minor (10) 459904CTGCTTTTCATTGAAAAGA 5-9-5 23 159 1954 1972 Major (10) 459912CTGCTTTTCGTTGAAAAGA 5-9-5 8 160

TABLE 12 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNPrs12506200 (nucleobases 3695 to 3715 of SEQ ID NO: 1) SEQ Start StopTarget % ID Site Site allele ISIS No. Sequence Motif inhibition NO145466 145485 n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 3695 3713Major (8) 459909 ACTAGGCCGGGCATGCTGG 5-9-5 48 161 3695 3713 Minor (8)459917 ACTAGGCTGGGCATGCTGG 5-9-5 35 162 3697 3715 Major (10) 459905AGACTAGGCCGGGCATGCT 5-9-5 33 163 3697 3715 Minor (10) 459913AGACTAGGCTGGGCATGCT 5-9-5 45 164

TABLE 13 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs762855(nucleobases 14437 to 14457 of SEQ ID NO: 1) SEQ Start Stop Target % IDSite Site allele ISIS No. Sequence Motif inhibition NO 145466 145485 n/a387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 14437 14455 Minor (8) 459910AAACAGCTGTTAGTTCCCA 5-9-5 27 165 14437 14455 Major (8) 459918AAACAGCCGTTAGTTCCCA 5-9-5 39 166 14439 14457 Minor (10) 459906AGAAACAGCTGTTAGTTCC 5-9-5 24 167 14439 14457 Major (10) 459914AGAAACAGCCGTTAGTTCC 5-9-5 28 168

TABLE 14 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs4690072(nucleobases 62147 to 62173 of SEQ ID NO: 1) SEQ Start Stop % ID SiteSite Target allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 62147 62165 Major (6) 460145GTGCTACCCAACCTTTCTG 5-9-5 62 169 62148 62166 Major (7) 460144AGTGCTACCCAACCTTTCT 5-9-5 61 170 62149 62167 Major (8) 460143CAGTGCTACCCAACCTTTC 5-9-5 65 171 62150 62168 Major (9) 460142ACAGTGCTACCCAACCTTT 5-9-5 83 172 62151 62169 Major (10) *435874CACAGTGCTACCCAACCTT 5-9-5 76 28 62151 62169 Major (10) 460022CACAGTGCTACCCAACCTT 4-10-5 75 28 62151 62169 Major (10) 460033CACAGTGCTACCCAACCTT 4-11-4 89 28 62151 62168 Major (9) 460063ACAGTGCTACCCAACCTT 4-9-5 77 173 62151 62169 Major (10) 460073CACAGTGCTACCCAACCTT 5-10-4 86 28 62151 62169 Major (10) 460093CACAGTGCTACCCAACCTT 5-8-6 61 28 62151 62169 Major (10) 460169CACAGTGCTACCCAACCTT 6-7-6 16 28 62151 62169 Major (10) 460188CACAGTGCTACCCAACCTT 6-8-5 53 28 62152 62168 Major (9) 459978ACAGTGCTACCCAACCT 2-9-6 87 174 62152 62167 Major (8) 459999CAGTGCTACCCAACCT 3-9-4 48 175 62152 62168 Major (9) 460012ACAGTGCTACCCAACCT 3-9-5 84 174 62152 62168 Major (9) 460052ACAGTGCTACCCAACCT 4-9-4 51 174 62152 62168 Major (9) 460083ACAGTGCTACCCAACCT 5-7-5 37 174 62152 62168 Major (9) 460103ACAGTGCTACCCAACCT 5-9-3 80 174 62152 62170 Major (11) 460137TCACAGTGCTACCCAACCT 5-9-5 65 176 62152 62168 Major (9) 460179ACAGTGCTACCCAACCT 6-9-2 67 174 62153 62167 Major (8) 459989CAGTGCTACCCAACC 3-9-3 60 177 62153 62167 Major (8) 460043CAGTGCTACCCAACC 4-7-4 24 177 62153 62171 Major (12) 460138ATCACAGTGCTACCCAACC 5-9-5 76 178 62154 62172 Major (13) 460139TATCACAGTGCTACCCAAC 5-9-5 68 179 62155 62173 Major (14) 460140ATATCACAGTGCTACCCAA 5-9-5 79 180

TABLE 15 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs2298969(nucleobases 125883 to 125911 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 125883 125901 Minor (5)460166 ATGCTGACTTGGGCCATTC 5-9-5 83 181 125884 125902 Minor (6) 460165GATGCTGACTTGGGCCATT 5-9-5 88 182 125885 125903 Minor (7) 460164GGATGCTGACTTGGGCCAT 5-9-5 68 183 125886 125904 Minor (8) 460163GGGATGCTGACTTGGGCCA 5-9-5 73 184 125887 125905 Minor (9) 460162AGGGATGCTGACTTGGGCC 5-9-5 88 185 125888 125906 Minor (10) *435890AAGGGATGCTGACTTGGGC 5-9-5 83 94 125888 125906 Minor (10) 460026AAGGGATGCTGACTTGGGC 4-10-5 90 94 125888 125906 Minor (10) 460037AAGGGATGCTGACTTGGGC 4-11-4 86 94 125888 125905 Minor (9) 460068AGGGATGCTGACTTGGGC 4-9-5 90 186 125888 125906 Minor (10) 460076AAGGGATGCTGACTTGGGC 5-10-4 90 94 125888 125906 Minor (10) 460096AAGGGATGCTGACTTGGGC 5-8-6 88 94 125888 125906 Minor (10) 460171AAGGGATGCTGACTTGGGC 6-7-6 87 94 125888 125906 Minor (10) 460190AAGGGATGCTGACTTGGGC 6-8-5 69 94 125889 125905 Minor (9) 459983AGGGATGCTGACTTGGG 2-9-6 80 187 125889 125904 Minor (8) 460005GGGATGCTGACTTGGG 3-9-4 80 284 125889 125905 Minor (9) 460016AGGGATGCTGACTTGGG 3-9-5 90 187 125889 125905 Minor (9) 460057AGGGATGCTGACTTGGG 4-9-4 86 187 125889 125905 Minor (9) 460087AGGGATGCTGACTTGGG 5-7-5 86 187 125889 125905 Minor (9) 460107AGGGATGCTGACTTGGG 5-9-3 79 187 125889 125907 Major (11) 460157CAAGGGATGCTGACTTGGG 5-9-5 88 188 125889 125905 Minor (9) 460181AGGGATGCTGACTTGGG 6-9-2 62 187 125890 125904 Minor (8) 459972GGGATGCTGACTTGG Uniform 18 189 125890 125904 Minor (8) 459992GGGATGCTGACTTGG 3-9-3 90 189 125890 125904 Minor (8) 460046GGGATGCTGACTTGG 4-7-4 59 189 125890 125908 Major (12) 460158CCAAGGGATGCTGACTTGG 5-9-5 79 190 125891 125909 Major (13) 460159GCCAAGGGATGCTGACTTG 5-9-5 82 191 125892 125910 Major (14) 460160TGCCAAGGGATGCTGACTT 5-9-5 87 192 125893 125911 Major (15) 460161CTGCCAAGGGATGCTGACT 5-9-5 78 193

TABLE 16 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs7685686(nucleobases 146781 to 146809 of SEQ ID NO: 1) SEQ Start Stop TargetISIS % ID Site Site allele No. Sequence Motif inhibition NO 145466145485 n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 146781 146799 Major(5) 460156 ATTGTCATCACCAGAAAAA 5-9-5 88 194 146782 146800 Major (6)460155 AATTGTCATCACCAGAAAA 5-9-5 89 195 146783 146801 Major (7) 460154AAATTGTCATCACCAGAAA 5-9-5 89 196 146784 146802 Major (8) 460153TAAATTGTCATCACCAGAA 5-9-5 93 197 146785 146803 Major (9) 460152ATAAATTGTCATCACCAGA 5-9-5 95 198 146786 146804 Major (10) *435879AATAAATTGTCATCACCAG 5-9-5 94 99 146786 146804 Major (10) 460024AATAAATTGTCATCACCAG 4-10-5 88 99 146786 146804 Major (10) 460035AATAAATTGTCATCACCAG 4-11-4 91 99 146786 146803 Major (9) 460065ATAAATTGTCATCACCAG 4-9-5 96 199 146786 146804 Major (10) 460074AATAAATTGTCATCACCAG 5-10-4 94 99 146786 146804 Major (10) 460095AATAAATTGTCATCACCAG 5-8-6 92 99 146786 146804 Major (10) 460170AATAAATTGTCATCACCAG 6-7-6 91 99 146786 146804 Major (10) 460189AATAAATTGTCATCACCAG 6-8-5 94 99 146787 146803 Major (9) 459981ATAAATTGTCATCACCA 2-9-6 85 200 146787 146802 Major (8) 460002TAAATTGTCATCACCA 3-9-4 86 201 146787 146803 Major (9) 460014ATAAATTGTCATCACCA 3-9-5 91 200 146787 146803 Major (9) 460055ATAAATTGTCATCACCA 4-9-4 90 200 146787 146803 Major (9) 460085ATAAATTGTCATCACCA 5-7-5 94 200 146787 146803 Major (9) 460104ATAAATTGTCATCACCA 5-9-3 93 200 146787 146805 Major (11) 460147TAATAAATTGTCATCACCA 5-9-5 91 202 146787 146803 Major (9) 460180ATAAATTGTCATCACCA 6-9-2 91 200 146788 146802 Major (8) 459970TAAATTGTCATCACC Uniform 9 203 146788 146802 Major (8) 459990TAAATTGTCATCACC 3-9-3 67 203 146788 146802 Major (8) 460045TAAATTGTCATCACC 4-7-4 84 203 146788 146806 Major (12) 460148TTAATAAATTGTCATCACC 5-9-5 88 204 146789 146807 Major (13) 460149ATTAATAAATTGTCATCAC 5-9-5 32 205 146790 146808 Major (14) 460150TATTAATAAATTGTCATCA 5-9-5 29 206 146791 146809 Major (15) 460151CTATTAATAAATTGTCATC 5-9-5 33 207

TABLE 17 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362331(nucleobases 155474 to 155502 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 155474 155492 Major (5)460136 CAGTAGATGAGGGAGCAGG 5-9-5 81 208 155475 155493 Major (6) 460135ACAGTAGATGAGGGAGCAG 5-9-5 84 209 155476 155494 Major (7) 460134CACAGTAGATGAGGGAGCA 5-9-5 87 210 155477 155495 Major (8) 460133ACACAGTAGATGAGGGAGC 5-9-5 85 211 155478 155496 Major (9) 460132CACACAGTAGATGAGGGAG 5-9-5 86 212 155479 155497 Major (10) *435870 GCACACAGTAGATGAGGGA 5-9-5 91 103 155479 155497 Major (10) 460019GCACACAGTAGATGAGGGA 4-10-5 92 103 155479 155497 Major (10) 460031GCACACAGTAGATGAGGGA 4-11-4 95 103 155479 155496 Major (9) 460061CACACAGTAGATGAGGGA 4-9-5 87 213 155479 155497 Major (10) 460071GCACACAGTAGATGAGGGA 5-10-4 94 103 155479 155497 Major (10) 460090GCACACAGTAGATGAGGGA 5-8-6 86 103 155479 155497 Major (10) 460168GCACACAGTAGATGAGGGA 6-7-6 84 103 155479 155497 Major (10) 460187GCACACAGTAGATGAGGGA 6-8-5 89 103 155480 155496 Major (9) 459977CACACAGTAGATGAGGG 2-9-6 90 214 155480 155495 Major (8) 459996ACACAGTAGATGAGGG 3-9-4 37 215 155480 155496 Major (9) 460009CACACAGTAGATGAGGG 3-9-5 90 214 155480 155496 Major (9) 460051CACACAGTAGATGAGGG 4-9-4 73 214 155480 155496 Major (9) 460081CACACAGTAGATGAGGG 5-7-5 77 214 155480 155496 Major (9) 460101CACACAGTAGATGAGGG 5-9-3 84 214 155480 155498 Major (11) 460127TGCACACAGTAGATGAGGG 5-9-5 89 216 155480 155496 Major (9) 460178CACACAGTAGATGAGGG 6-9-2 92 214 155481 155495 Major (8) 459967ACACAGTAGATGAGG Uniform 81 217 155481 155495 Major (8) 459987ACACAGTAGATGAGG 3-9-3 18 217 155481 155495 Major (8) 460041ACACAGTAGATGAGG 4-7-4 54 217 155481 155499 Major (12) 460128GTGCACACAGTAGATGAGG 5-9-5 73 218 155482 155500 Major (13) 460129AGTGCACACAGTAGATGAG 5-9-5 86 219 155483 155501 Major (14) 460130AAGTGCACACAGTAGATGA 5-9-5 60 220 155484 155502 Major (15) 460131GAAGTGCACACAGTAGATG 5-9-5 73 221

TABLE 18 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362306(nucleobases 181739 to 181767 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 181739 181757 Major (5)460126 GCTGCAACCTGGCAACAAC 5-9-5 87 222 181740 181758 Major (6) 460125AGCTGCAACCTGGCAACAA 5-9-5 70 223 181741 181759 Major (7) 460123CAGCTGCAACCTGGCAACA 5-9-5 83 224 181742 181760 Major (8) 460121GCAGCTGCAACCTGGCAAC 5-9-5 47 225 181743 181761 Major (9) 460118AGCAGCTGCAACCTGGCAA 5-9-5 75 226 181744 181762 Major (10) *435869 GAGCAGCTGCAACCTGGCA 5-9-5 91 149 181744 181762 Major (10) 460018GAGCAGCTGCAACCTGGCA 4-10-5 86 149 181744 181762 Major (10) 460028GAGCAGCTGCAACCTGGCA 4-11-4 89 149 181744 181761 Major (9) 460058AGCAGCTGCAACCTGGCA 4-9-5 85 227 181744 181762 Major (10) 460069GAGCAGCTGCAACCTGGCA 5-10-4 91 149 181744 181762 Major (10) 460089GAGCAGCTGCAACCTGGCA 5-8-6 54 149 181744 181762 Major (10) 460167GAGCAGCTGCAACCTGGCA 6-7-6 85 149 181744 181762 Major (10) 460186GAGCAGCTGCAACCTGGCA 6-8-5 84 149 181745 181761 Major (9) 459975AGCAGCTGCAACCTGGC 2-9-6 86 228 181745 181760 Major (8) 459995GCAGCTGCAACCTGGC 3-9-4 87 229 181745 181761 Major (9) 460008AGCAGCTGCAACCTGGC 3-9-5 83 228 181745 181761 Major (9) 460049AGCAGCTGCAACCTGGC 4-9-4 88 228 181745 181761 Major (9) 460079AGCAGCTGCAACCTGGC 5-7-5 46 228 181745 181761 Major (9) 460099AGCAGCTGCAACCTGGC 5-9-3 44 228 181745 181763 Major (11) 460108AGAGCAGCTGCAACCTGGC 5-9-5 50 230 181745 181761 Major (9) 460177AGCAGCTGCAACCTGGC 6-9-2 67 228 181746 181760 Major (8) 459966GCAGCTGCAACCTGG Uniform 26 231 181746 181760 Major (8) 459985GCAGCTGCAACCTGG 3-9-3 69 231 181746 181760 Major (8) 460039GCAGCTGCAACCTGG 4-7-4 56 231 181746 181764 Major (12) 460110AAGAGCAGCTGCAACCTGG 5-9-5 75 232 181747 181765 Major (13) 460113CAAGAGCAGCTGCAACCTG 5-9-5 36 233 181748 181766 Major (14) 460115GCAAGAGCAGCTGCAACCT 5-9-5 78 234 181749 181767 Major (15) 460117TGCAAGAGCAGCTGCAACC 5-9-5 73 235

TABLE 19 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs1006798(nucleobases 198015 to 198035 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 98 6 198015 198033 Minor (8)459911 ACCATGATATCTCCAGCAC 5-9-5 33 236 198015 198033 Minor (8) 459919ACCATGACATCTCCAGCAC 5-9-5 26 237 198017 198035 Major (10) 459907CCACCATGATATCTCCAGC 5-9-5 32 238 198017 198035 Minor (10) 459915CCACCATGACATCTCCAGC 5-9-5 51 239

Example 6 Dose-Dependent Antisense Inhibition of Human Huntingtin mRNALevels in Coriell Fibroblast Cell Lines

Gapmers from the studies described in Example 5 were selected and testedat various doses in GM04281, GM02171, and GM02173B cell lines. Each cellline was plated at a density of 25,000 cells per well and transfectedusing electroporation with 750 nM, 1,500 nM, 3,000 nM, 6,000 nM, and12,000 nM concentrations of antisense oligonucleotide, as specified inTables 20, 21, and 22. After a treatment period of approximately 16hours, RNA was isolated from the cells and HTT mRNA levels were measuredby quantitative real-time PCR. Human HTT primer probe set RTS2617 wasused to measure mRNA levels. HTT mRNA levels were adjusted according tototal RNA content, as measured by RIBOGREEN. Results are presented aspercent inhibition of HTT mRNA, relative to untreated control cells.IC₅₀ values are also provided in Tables 20, 21, and 22.

TABLE 20 Dose-dependent antisense inhibition of human HTT in GM04281cells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 56 81 89 96 98 0.6 435869 38 49 66 86 91 1.4 435874 33 27 37 4962 8.4 435879 42 55 73 86 96 1.1 435890 39 51 74 83 89 1.3 459978 29 3351 69 86 2.5 459992 14 27 51 54 84 3.2 460012 15 24 54 70 81 3.1 4600163 36 48 71 77 3.3 460019 54 59 74 87 94 0.7 460026 48 47 71 79 88 0.8460028 39 38 73 77 87 1.4 460031 44 62 72 87 92 0.9 460033 11 38 52 6487 3.0 460065 43 54 74 89 96 1.1 460068 47 28 63 76 90 2.6 460069 38 5065 77 91 1.4 460071 53 61 80 89 93 0.6 460073 16 39 42 58 75 4.0 46007626 47 54 70 86 2.1 460085 48 60 79 89 94 0.8 460140 6 24 44 44 64 6.6460142 2 38 46 46 68 4.8 460152 35 61 76 92 94 1.2 460157 51 36 53 74 892.6 460162 64 41 71 76 85 2.1 460165 41 50 56 76 84 1.5

TABLE 21 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 53 66 88 96 98 0.7 435869 4 20 36 63 86 3.9 435870 25 39 48 62 832.8 435874 12 20 18 27 37 >12.0 435879 10 7 11 42 51 10.6 435890 10 2329 29 55 9.2 459978 15 7 6 29 52 12.7 459992 11 19 26 39 62 8.7 460012 33 10 19 41 >12.0 460016 0 14 12 22 48 >12.0 460019 27 21 41 60 73 4.4460026 9 25 30 46 58 7.8 460028 24 8 32 54 77 5.3 460031 8 25 42 60 833.8 460033 11 25 30 40 75 4.1 460065 11 16 11 31 53 10.3 460068 15 13 3944 53 8.8 460069 17 28 37 60 79 3.9 460071 16 36 58 70 88 2.6 460073 519 24 33 56 8.7 460076 19 29 44 54 83 3.3 460085 10 15 17 28 31 >12.0460140 8 22 22 28 47 >12.0 460142 11 24 28 36 38 >12.0 460152 14 21 8 2544 22 460157 22 21 29 44 66 6.7 460162 24 55 52 62 82 2.8 460165 14 3450 69 81 3.1

TABLE 22 Dose-dependent antisense inhibition of human HTT in GM02173Bcells ISIS 750 IC₅₀ No. nM 1,500 nM 3,000 nM 6,000 nM 12,000 nM (μM)387916 37 63 86 88 98 1.0 435869 10 20 43 70 85 3.5 435870 24 24 56 7287 2.3 435874 0 11 12 30 44 >12.0 435879 4 17 43 64 74 4.3 435890 31 2954 57 69 4.4 459978 7 13 17 35 64 8.4 459992 18 15 30 51 71 5.7 460012 010 24 37 72 7.1 460016 15 5 30 38 59 9.5 460019 10 32 51 65 87 3.1460026 0 34 21 55 65 6.4 460028 0 14 31 51 77 5.2 460031 0 31 53 71 883.2 460033 11 8 6 52 84 5.0 460065 19 37 53 58 74 3.6 460068 17 11 31 5969 5.5 460069 11 21 37 55 75 4.6 460071 6 42 61 83 88 2.6 460073 7 13 1949 66 6.3 460076 27 31 49 43 81 2.9 460085 17 34 51 54 68 4.4 460140 0 228 18 46 >12.0 460142 2 32 37 42 59 7.6 460152 17 32 35 51 66 5.5 4601579 34 38 52 74 4.5 460162 22 45 57 65 79 2.5 460165 5 45 52 72 84 3.2

Example 7 Antisense Inhibition of Human HTT in GM04281 Cells and GM02171Cells

Additional antisense oligonucleotides were designed based on the gapmersselected from studies described in Example 2. These oligonucleotideswere designed by creating gapmers shifted slightly upstream anddownstream (i.e. “microwalk”) of the original gapmers from Table 4.

The gapmers were tested in the GM04281 and the GM02171 cell lines.Cultured GM04281 or GM02171 cells at a density of 25,000 cells per wellwere transfected using electroporation with 10,000 nM antisenseoligonucleotide. After a treatment period of approximately 24 hours, RNAwas isolated from the cells and HTT mRNA levels were measured byquantitative real-time PCR using primer probe set RTS2617. HTT mRNAlevels were adjusted according to total RNA content, as measured byRIBOGREEN. Results are presented as percent inhibition of HTT mRNA,relative to untreated control cells.

The gapmers, from which the newly designed oligonucleotides werederived, were also included in the assay. These parent gapmers, ISIS435294, ISIS 435295, ISIS 435301, ISIS 435303, ISIS 435304, ISIS 435305,ISIS 435308, ISIS 435330, ISIS 435331, ISIS 435337, ISIS 435339, ISIS435340, ISIS 435341, ISIS 435344, ISIS 435862, ISIS 435863, ISIS 435864,ISIS 435866, ISIS 435867, ISIS 435868, ISIS 435871, ISIS 435873, ISIS435875, ISIS 435876, ISIS 435878, ISIS 435880, ISIS 435881, ISIS 435882,ISIS 435884, ISIS 435890, and ISIS 435897 are marked with an asterisk(*) in the table. ISIS 387916 was included in the study as a benchmarkoligonucleotide against which the potency of the antisenseoligonucleotides targeting nucleotides overlapping each SNP positioncould be compared.

The chimeric antisense oligonucleotides in Tables 23-48 were designed as5-9-5 MOE gapmers. The 5-9-5 gapmers are 19 nucleotides in length,wherein the central gap segment is comprised of nine 2′-deoxynucleotidesand is flanked on both 5′ and 3′ directions by wings comprising 5nucleotides each. Each nucleotide in the 5′ wing segment and eachnucleotide in the 3′ wing segment has a 2′-MOE modification. Theinternucleoside linkages throughout each gapmer are phosphorothioate(P═S) linkages. All cytosine nucleobases throughout each gapmer are5-methylcytosines.

The gapmers are organized in Tables 23-48, according to the SNP sitethey target. “Start site” indicates the 5′-most nucleotide to which thegapmer is targeted. “Stop site” indicates the 3′-most nucleotide towhich the gapmer is targeted. ‘Target allele’ indicates whether thegapmer is targeted to the major or the minor allele. The number inparentheses indicates the position on the oligonucleotide opposite tothe SNP position.

TABLE 23 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs3856973(nucleobases 19815 to 19835 of SEQ ID NO: 1) % % inhibition inhibitionStart Stop Target in in SEQ Site Site ISIS No allele Sequence GM04281GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 619815 19833 *435330 Major (8) TAACACTCGATTAACCCTG 88 31 8 19816 19834476441 Major (9) TTAACACTCGATTAACCCT 88 0 240 19817 19835 *435294 Major(10) GTTAACACTCGATTAACCC 72 30 10

TABLE 24 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs2285086(nucleobases 28901 to 28921 of SEQ ID NO: 1) % % inhibition inhibitionStart Stop Target in in SEQ Site Site ISIS No allele Sequence GM04281GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 628901 28919 463570 Major (8) TAGTTCATCCCAGTGAGAA 66 12 241 28902 28920463573 Major (9) CTAGTTCATCCCAGTGAGA 66 36 242 28903 28921 *435864 Major(10) GCTAGTTCATCCCAGTGAG 40 18 12

TABLE 25 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs7659144(nucleobases 37963 to 37983 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 3796337981 476462 Major (8) GAAATGGGTTTTTCCACAT 38 0 243 37964 37982 476439Major (9) GGAAATGGGTTTTTCCACA 80 45 244 37965 37983 *435878 Major (10)TGGAAATGGGTTTTTCCAC 76 3 14

TABLE 26 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNPrs16843804 (nucleobases 44032 to 44052 of SEQ ID NO: 1) % % inhibitioninhibition SEQ Start Stop Target in in ID Site Site ISIS No alleleSequence GM04281 GM02171 NO 145466 145485 387916 n/aTCTCTATTGCACATTCCAAG 100 99 6 44032 44050 476471 Major (8)TAACCGTGGCATGGGCAGT 82 53 245 44033 44051 476452 Major (9)TTAACCGTGGCATGGGCAG 84 44 246 44034 44052 *435863 Major (10)TTTAACCGTGGCATGGGCA 89 89 16

TABLE 27 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs2024115(nucleobases 44210 to 44230 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 4421044228 *435331 Major (8) TTCAAGCTAGTAACGATGC 84 20 18 44211 44229 476447Major (9) CTTCAAGCTAGTAACGATG 87 57 247 44212 44230 *435295 Major (10)ACTTCAAGCTAGTAACGAT 85 67 20

TABLE 28 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNPrs10015979 (nucleobases 49084 to 49104 of SEQ ID NO: 1) % % inhibitioninhibition SEQ Start Stop Target in in ID Site Site ISIS No alleleSequence GM04281 GM02171 NO 145466 145485 387916 n/aTCTCTATTGCACATTCCAAG 100 99 6 49084 49102 476470 Major (8)AGCTAGGTTAAAGAGTCAC 55 74 248 49085 49103 476450 Major (9)CAGCTAGGTTAAAGAGTCA 44 5 249 49086 49104 *435862 Major (10)GCAGCTAGGTTAAAGAGTC 56 49 22

TABLE 29 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs7691627(nucleobases 51052 to 51072 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 5105251070 476467 Major (8) TAAGAAACACAATCAAAGA 45 21 250 51053 51071 476445Major (9) ATAAGAAACACAATCAAAG 34 1 251 51054 51072 *435880 Major (10)AATAAGAAACACAATCAAA 68 7 24

TABLE 30 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs6446723(nucleobases 66455 to 66475 of SEQ ID NO: 1) % % inhibition inhibitionStart Stop Target in in SEQ Site Site ISIS No allele Sequence GM04281GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 666455 66473 476463 Major (8) ATTTTCTAGACTTTATGAT 37 7 252 66456 66474476440 Major (9) AATTTTCTAGACTTTATGA 57 0 253 66457 66475 *435875 Major(10) TAATTTTCTAGACTTTATG 42 0 30

TABLE 31 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and a chimeric antisense oligonucleotide targeted to SNP rs363064(nucleobases 81053 to 81071 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 8105381071 476461 Major (9) GAGAATACGGGTAACATTT 87 62 254

TABLE 32 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNPrs11731237 (nucleobases 91455 to 91475 of SEQ ID NO: 1) % % inhibitioninhibition Start Stop Target in in SEQ Site Site ISIS No allele SequenceGM04281 GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 10099 6 91455 91473 476468 Major (8) TGGGCAGGAAGGACTGAAC 58 56 255 9145691474 476448 Major (9) GTGGGCAGGAAGGACTGAA 61 69 256 91457 91475 *435884Major (10) GGTGGGCAGGAAGGACTGA 59 49 68

TABLE 33 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs4690073(nucleobases 99792 to 99812 of SEQ ID NO: 1) % % inhibition inhibitionStart Stop Target in in SEQ Site Site ISIS No allele Sequence GM04281GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 699792 99810 *435337 Major (8) CCTAAATCAATCTACAAGT 69 7 70 99793 99811476446 Major (9) CCCTAAATCAATCTACAAG 61 0 257 99794 99812 *435301 Major(10) TCCCTAAATCAATCTACAA 63 1 72

TABLE 34 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNPrs34315806 (nucleobases 101676 to 101696 of SEQ ID NO: 1) % % inhibitioninhibition Start Stop Target in in SEQ Site Site ISIS No allele SequenceGM04281 GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 10099 6 101676 101694 463569 Major (8) CTTTTCCGTGCTGTTCTGA 96 95 258 101677101695 463572 Major (9) ACTTTTCCGTGCTGTTCTG 93 91 259 101678 101696463567 Major (10) AACTTTTCCGTGCTGTTCT 98 97 260

TABLE 35 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs363099(nucleobases 101698 to 101718 of SEQ ID NO: 1) % % inhibition inhibitionStart Stop Target in in SEQ Site Site ISIS No allele Sequence GM04281GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6101698 101716 *435339 Major (8) CTGAGCGGAGAAACCCTCC 94 85 80 101699101717 476458 Major (9) GCTGAGCGGAGAAACCCTC 92 79 261 101700 101718*435303 Major (10) GGCTGAGCGGAGAAACCCT 96 93 82

TABLE 36 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs363096(nucleobases 119663 to 119683 of SEQ ID NO: 1) % % inhibition inhibitionStart Stop Target in in SEQ Site Site ISIS No allele Sequence GM04281GM02171 ID NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6119663 119681 *435340 Major (8) TTCCCTAAAAACAAAAACA 42 21 85 119664119682 476451 Major (9) ATTCCCTAAAAACAAAAAC 0 0 262 119665 119683*435304 Major (10) GATTCCCTAAAAACAAAAA 41 27 87

TABLE 37 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs2298967(nucleobases 125389 to 125409 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Stop Target in in ID Start Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 125389125407 *435341 Major (8) CTTTTCTATTGTCTGTCCC 83 65 89 125390 125408476459 Major (9) GCTTTTCTATTGTCTGTCC 89 82 263 125391 125409 *435305Major (10) TGCTTTTCTATTGTCTGTC 92 85 91

TABLE 38 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and a chimeric antisense oligonucleotide targeted to SNPrs2298969 (nucleobases 125888 to 125906 of SEQ ID NO: 1) % % inhibitioninhibition SEQ Start Stop Target in in ID Site Site ISIS No alleleSequence GM04281 GM02171 NO 145466 145485 387916 n/aTCTCTATTGCACATTCCAAG 100 99 6 125888 125906 *435890 Minor (10)AAGGGATGCTGACTTGGGC 91 64 94

TABLE 39 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs6844859(nucleobases 130128 to 130148 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 130128130146 476466 Major (8) CTTCCTCACTGAGGATGAA 87 64 264 130129 130147476444 Major (9) CCTTCCTCACTGAGGATGA 92 77 265 130130 130148 *435876Major (10) ACCTTCCTCACTGAGGATG 94 87 95

TABLE 40 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs363092(nucleobases 135671 to 135691 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop in in ID Site Site ISIS No Target allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 135671135689 476464 Major (8) AACCACTTTGGGATGAATA 51 71 266 135672 135690476442 Major (9) AAACCACTTTGGGATGAAT 58 59 267 135673 135691 *435897Minor (10) CAAACCACTTTGGGATGAA 48 78 98

TABLE 41 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs363088(nucleobases 149972 to 149992 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Target in in ID Start Site Stop Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 149972149990 476476 Major (8) ACAGCTATCTTCTCATCAA 90 65 268 149973 149991476460 Major (9) CACAGCTATCTTCTCATCA 86 39 269 149974 149992 *435871Major (10) TCACAGCTATCTTCTCATC 91 54 101

TABLE 42 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs916171(nucleobases 156457 to 156477 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop in in ID Site Site ISIS No Target allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 156457156475 476465 Major (8) GAACAAAGAGAAGAATTTC 38 0 270 156458 156476476443 Major (9) AGAACAAAGAGAAGAATTT 58 0 271 156459 156477 *435881Major (10) CAGAACAAAGAGAAGAATT 59 16 105

TABLE 43 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362275(nucleobases 164244 to 164264 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop in in ID Site Site ISIS No Target allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 164244164262 476473 Major (8) GAAGCCTGATAAAATCTCT 83 51 272 164245 164263476454 Major (9) AGAAGCCTGATAAAATCTC 79 61 273 164246 164264 *435868Major (10) AAGAAGCCTGATAAAATCT 69 56 111

TABLE 44 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362273(nucleobases 167061 to 167081 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 167061167079 463568 Major (8) TGATCTGTAGCAGCAGCTT 96 78 274 167062 167080463571 Major (9) TTGATCTGTAGCAGCAGCT 95 86 275 167063 167081 463566Major (10) GTTGATCTGTAGCAGCAGC 94 78 276

TABLE 45 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362272(nucleobases 174622 to 174642 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 174622174640 *435344 Major (8) TAGAGGACGCCGTGCAGGG 78 63 120 174623 174641476456 Major (9) ATAGAGGACGCCGTGCAGG 87 60 277 174624 174642 *435308Major (10) CATAGAGGACGCCGTGCAG 76 48 122

TABLE 46 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362271(nucleobases 175160 to 175180 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop in in ID Site Site ISIS No Target allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 175160175178 476472 Major (8) GTGTGTACAGAACCTGCCG 85 52 278 175161 175179476453 Major (9) CGTGTGTACAGAACCTGCC 88 69 279 175162 175180 *435867Major (10) ACGTGTGTACAGAACCTGC 91 80 125

TABLE 47 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs3775061(nucleobases 178396 to 178416 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Target in in ID Site Site ISIS No allele Sequence GM04281GM02171 NO 145466 145485 387916 n/a TCTCTATTGCACATTCCAAG 100 99 6 178396178414 476475 Major (8) TTCAGAATGCCTCATCTGG 61 1 280 178397 178415476457 Major (9) GTTCAGAATGCCTCATCTG 80 50 281 178398 178416 *435873Major (10) TGTTCAGAATGCCTCATCT 80 43 127

TABLE 48 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362296(nucleobases 186649 to 1786669 of SEQ ID NO: 1) % % inhibitioninhibition SEQ Start Stop Target in in ID Site Site ISIS No alleleSequence GM04281 GM02171 NO 145466 145485 387916 n/aTCTCTATTGCACATTCCAAG 100 99 6 186649 186667 476469 Major (8)GGACAGGGTGTGCTCTCCG 80 58 282 186650 186668 476449 Major (9)GGGACAGGGTGTGCTCTCC 80 64 283 186651 186669 *435882 Major (10)GGGGACAGGGTGTGCTCTC 61 61 155

Example 8 Dose-Dependent Antisense Inhibition of Human Huntingtin mRNALevels in Coriell Fibroblast Cell Lines

Gapmers from the studies described in Example 7 were selected and testedat various doses in GM04281, GM02171, and GM02173B cell lines. Each cellline was plated at a density of 25,000 cells per well and transfectedusing electroporation with 750 nM, 1,500 nM, 3,000 nM, 6,000 nM, and12,000 nM concentrations of antisense oligonucleotide, as specified inTables 49, 50, and 51. After a treatment period of approximately 16hours, RNA was isolated from the cells and HTT mRNA levels were measuredby quantitative real-time PCR. Human HTT primer probe set RTS2617 wasused to measure mRNA levels. HTT mRNA levels were adjusted according tototal RNA content, as measured by RIBOGREEN. Results are presented aspercent inhibition of HTT mRNA, relative to untreated control cells.IC₅₀ values are also provided in Tables 49, 50, and 51.

TABLE 49 Dose-dependent antisense inhibition of human HTT in GM04281cells ISIS 750 1500 3000 6000 12000 IC₅₀ No. nM nM nM nM nM (μM) 38791667 88 95 97 99 <0.8 463566 25 65 79 88 95 1.5 463567 34 73 90 93 98 1.1463568 33 56 75 87 92 1.3 463571 32 21 70 90 93 1.4 476441 11 27 50 7087 3.1 476444 20 31 68 49 93 2.3 476449 4 28 34 47 77 4.9 476453 21 2148 73 85 2.7 476455 5 19 34 56 80 4.6 476458 36 72 83 93 96 1.1 47645923 59 75 85 91 1.5 476469 17 27 47 47 67 5.5 476473 0 6 32 50 68 6.2476476 3 7 32 53 86 4.9

TABLE 50 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 750 1500 3000 6000 12000 IC₅₀ No. nM nM nM nM nM (μM) 38791659 79 93 98 98 <0.8 463566 4 33 42 62 79 3.8 463567 38 41 69 85 94 1.5463568 21 26 41 58 64 4.8 463571 8 23 56 63 75 3.7 476441 0 13 7 012 >12.0 476444 11 0 0 67 59 8.8 476449 4 27 37 51 63 5.8 476453 6 40 4051 73 4.9 476455 32 15 18 47 61 7.8 476458 42 54 71 86 84 1.2 476459 2238 70 44 73 4.3 476469 7 24 30 56 58 7.8 476473 4 10 15 33 43 >12.0476476 5 16 18 23 41 >12.0

TABLE 51 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 750 1500 3000 6000 12000 IC₅₀ No. nM nM nM nM nM (μM) 38791666 89 95 97 99 <0.8 463566 32 55 76 77 93 1.3 463567 51 61 87 94 97 0.7463568 26 23 72 87 94 1.6 463571 32 34 60 86 94 1.9 476441 18 18 27 4744 >12.0 476444 15 0 31 51 58 7.1 476449 27 33 56 80 81 2.6 476453 24 2855 75 83 2.7 476455 24 26 52 55 73 3.7 476458 63 77 87 89 94 0.2 47645937 55 56 62 86 1.5 476469 22 41 40 63 76 2.9 476473 7 28 33 51 73 5.0476476 11 29 26 55 69 4.6

Example 9 Antisense Inhibition of Human HTT in GM04281 Cells byOligonucleotides Designed by Microwalk

Additional gapmers were designed based on the gapmers selected fromstudies described in Example 4. These gapmers were designed by creatinggapmers shifted slightly upstream and downstream (i.e. “microwalk”) ofthe original gapmers from Tables 8, 9, and 10. Gapmers were also createdwith 3-9-3 or 5-9-5 motifs, and with constrained 6(S)—CH₃-bicyclicnucleic acid (BNA) molecules at various nucleoside positions.

These gapmers were tested in vitro. Cultured GM04281 cells at a densityof 25,000 cells per well were transfected using electroporation with5,000 nM antisense oligonucleotide. After a treatment period ofapproximately 24 hours, RNA was isolated from the cells and HTT mRNAlevels were measured by quantitative real-time PCR. HTT mRNA levels wereadjusted according to total RNA content, as measured by RIBOGREEN.Results are presented as percent inhibition of HTT mRNA, relative tountreated control cells.

The chimeric antisense oligonucleotides in Tables 52-56 were designed as3-9-3 or 5-9-5 gapmers. The parent gapmers, ISIS 435869, ISIS 435870,ISIS 435874, ISIS 435879, and ISIS 435890, from which the newly designedgapmers were derived are marked with an asterisk (*) in the table. ISIS387916 was included in the study as a benchmark oligonucleotide againstwhich the potency of the antisense oligonucleotides targetingnucleotides overlapping each SNP position could be compared.

The 3-9-3 gapmers are 15 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleosides and is flanked on both5′ and 3′ directions by wings comprising 3 sugar modified nucleosideseach.

The 5-9-5 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleosides and is flanked on both5′ and 3′ directions by wings comprising 5 sugar modified nucleosideseach.

The internucleoside linkages throughout each gapmer are phosphorothioate(P═S) linkages. All cytosine nucleobases throughout each gapmer are5-methylcytosines. Bolded and underlined nucleotides in Tables 52-56indicate the positions of the 6(S)—CH₃-BNA molecules (e.g. cEtmolecules) in each gapmer. Italicized nucleotides are MOE subunits.

“Start site” indicates the 5′-most nucleotide to which the gapmer istargeted. “Stop site” indicates the 3′-most nucleotide to which thegapmer is targeted. ‘Target allele’ indicates whether the gapmer istargeted to the major or the minor allele. The number in parenthesesindicates the position on the oligonucleotide opposite to the SNPposition.

TABLE 52 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs4690072(nucleobases 62147 to 62173 of SEQ ID NO: 1) Start Stop Target % SEQSite Site allele ISIS No. Sequence Motif inhibition ID NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 97 6 62147 62165 Major (6) 460266GTGCT A CCCAACCTTTCTG 5-9-5 63 169 62151 62169 Major (10) *435874CACAGTGCTACCCAACCTT 5-9-5 50 28 62151 62169 Major (10) 460213 CACAGTGCTA CCCAACCTT 5-9-5 22 28 62151 62169 Major (10) 460220 CACAGTGC TACCCAACCTT 5-9-5 24 28 62151 62169 Major (10) 460221 CACAGTGCTA CCCAACCTT 5-9-5 28 28 62153 62167 Major (8) 460208 C AG TGCTACCCA AC C3-9-3 81 177 62155 62173 Major (14) 460267 ATATCACAGTGCT A CCCAA 5-9-537 180

TABLE 53 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs2298969(nucleobases 125884 to 125910 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 97 6 125884 125902 Minor (6)460233 GATGC T GACTTGGGCCATT 5-9-5 76 182 125888 125906 Minor (10)*435890 AAGGGATGCTGACTTGGGC 5-9-5 75 94 125888 125906 Minor (10) 460215AAGGGATGC T GACTTGGGC 5-9-5 26 94 125888 125906 Minor (10) 460224AAGGGATG C TGACTTGGGC 5-9-5 38 94 125888 125906 Minor (10) 460225AAGGGATGCT G ACTTGGGC 5-9-5 49 94 125890 125904 Minor (8) 460210 G GGATGCTGACT TG G 3-9-3 97 189 125892 125910 Minor (14) 460229TGCCAAGGGATGC T GACTT 5-9-5 60 192

TABLE 54 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs7685686(nucleobases 146782 to 146808 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 97 6 146782 146800 Major (6)460232 AATT G TCATCACCAGAAAA 5-9-5 82 195 146786 146804 Major (10)*435879 AATAAATTGTCATCACCAG 5-9-5 84 99 146786 146804 Major (10) 460214AATAAATTG T CATCACCAG 5-9-5 33 99 146786 146804 Major (10) 460222AATAAATT G TCATCACCAG 5-9-5 87 99 146786 146804 Major (10) 460223AATAAATTGT C ATCACCAG 5-9-5 75 99 146788 146802 Major (8) 460209 T AAATTGTCATC AC C 3-9-3 96 203 146790 146808 Major (14) 460228TATTAATAAATTG T CATCA 5-9-5 0 206

TABLE 55 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362331(nucleobases 155475 to 155501 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 97 6 155475 155493 Major (6)460231 ACAGT A GATGAGGGAGCAG 5-9-5 88 209 155479 155497 Major (10)*435870 GCACACAGTAGATGAGGGA 5-9-5 86 103 155479 155497 Major (10) 460212GCACACAGT A GATGAGGGA 5-9-5 89 103 155479 155497 Major (10) 460218GCACACAG T AGATGAGGGA 5-9-5 90 103 155479 155497 Major (10) 460219GCACACAGTA G ATGAGGGA 5-9-5 88 103 155481 155495 Major (8) 460207 A CACAGTAGATG AG G 3-9-3 89 217 155483 155501 Major (14) 460227AAGTGCACACAGT A GATGA 5-9-5 45 220

TABLE 56 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362306(nucleobases 181740 to 181766 of SEQ ID NO: 1) SEQ Start Stop Target %ID Site Site allele ISIS No. Sequence Motif inhibition NO 145466 145485n/a 387916 TCTCTATTGCACATTCCAAG 5-10-5 97 6 181740 181758 Major (6)460230 AGCTG C AACCTGGCAACAA 5-9-5 66 223 181744 181762 Major (10)*435869 GAGCAGCTGCAACCTGGCA 5-9-5 69 149 181744 181762 Major (10) 460211GAGCAGCTG C AACCTGGCA 5-9-5 22 149 181744 181762 Major (10) 460216GAGCAGCT G CAACCTGGCA 5-9-5 18 149 181744 181762 Major (10) 460217GAGCAGCTGC A ACCTGGCA 5-9-5 56 149 181746 181760 Major (8) 460206 G CAGCTGCAACC TG G 3-9-3 83 231 181748 181766 Major (14) 460226GCAAGAGCAGCTG C AACCT 5-9-5 51 234

Example 10 Dose-Dependent Antisense Inhibition of Human Huntingtin mRNALevels in Coriell Fibroblast Cell Lines

Gapmers from studies described in Example 9 were selected and tested atvarious doses in GM04281, GM02171 and GM02173B cell lines. Each cellline was plated at a density of 25,000 cells per well and transfectedusing electroporation with 312.5 nM, 625 nM, 1,250 nM, 2,500 nM, 5,000nM and 10,000 nM concentrations of antisense oligonucleotide, asspecified in Tables 75, 58, and 59. After a treatment period ofapproximately 16 hours, RNA was isolated from the cells and HTT mRNAlevels were measured by quantitative real-time PCR. Human HTT primerprobe set RTS2617 was used to measure mRNA levels. HTT mRNA levels wereadjusted according to total RNA content, as measured by RIBOGREEN.Results are presented as percent inhibition of HTT mRNA, relative tountreated control cells. IC₅₀ values are also provided in Tables 57, 58,and 59.

TABLE 57 Dose-dependent antisense inhibition of human HTT in GM04281cells ISIS 312.5 625 1,250 2,500 5,000 10,000 IC₅₀ No. nM nM nM nM nM nM(μM) 387916 26 49 68 86 94 97 0.7 435869 0 0 23 48 62 82 3.2 435870 1538 50 65 85 88 1.3 435874 14 22 32 49 65 73 2.7 435879 0 17 40 61 83 941.8 435890 5 13 37 56 70 82 2.3 460206 10 18 37 52 66 85 2.3 460207 2027 50 65 80 91 1.4 460208 21 34 51 63 70 79 1.5 460209 52 74 89 94 94 950.2 460210 34 61 84 91 97 98 0.5 460212 13 31 50 62 75 82 1.6 460218 1427 50 63 78 86 1.8 460219 9 32 42 64 77 87 1.6 460222 19 21 42 57 73 781.7 460231 12 24 41 57 71 84 1.9 460233 16 28 59 66 72 74 1.8 460266 417 32 48 60 75 2.9

TABLE 58 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 312.5 625 1,250 2,500 5,000 10,000 IC₅₀ No. nM nM nM nM nM nM(μM) 387916 32 56 77 89 95 97 0.7 435869 0 6 22 40 69 84 2.9 435870 1519 32 51 68 77 2.4 435874 0 5 1 17 17 30 >10.0 435879 0 8 0 16 36 4715.3 435890 14 16 19 19 39 57 9.3 460206 5 13 33 41 68 80 2.7 460207 1310 22 22 33 39 45.6 460208 13 15 11 11 15 53 10.8 460209 8 27 46 70 8086 1.6 460210 19 37 55 75 88 96 1.1 460212 8 23 30 43 57 74 2.2 46021815 26 27 36 52 78 3.2 460219 16 17 32 44 69 76 2.5 460222 14 3 0 0 130 >10.0 460231 6 8 13 16 33 56 10.4 460233 27 30 39 46 61 73 2.4 4602660 15 20 15 18 34 >10.0

TABLE 59 Dose-dependent antisense inhibition of human HTT in GM02173Bcells ISIS 312.5 625 1,250 2,500 5,000 10,000 IC₅₀ No. nM nM nM nM nM nM(μM) 387916 22 47 76 88 96 98 0.7 435869 10 0 16 38 59 76 3.9 435870 2236 44 58 69 81 2.0 435874 11 6 25 23 32 42 >10.0 435879 0 9 21 30 52 684.8 435890 12 16 30 31 48 66 4.5 460206 11 13 18 35 59 74 3.5 460207 1525 30 37 42 66 4.3 460208 5 14 27 32 52 51 9.0 460209 27 49 61 79 81 740.8 460210 19 40 61 77 89 95 1.0 460212 0 19 32 32 61 78 2.9 460218 4 1726 38 64 82 3.0 460219 5 6 26 47 68 84 2.9 460222 13 19 23 30 35 50 16.1460231 7 33 25 35 54 77 3.7 460233 11 20 37 52 68 69 2.3 460266 12 6 1021 25 47 >10.0

Example 11 Dose-Dependent Antisense Inhibition of Human HTT in GM04281and GM02171 Cells by Oligonucleotides Designed by Microwalk

Additional gapmers were designed based on the gapmers selected fromstudies described in Example 10. These gapmers were designed by creatinggapmers shifted slightly upstream and downstream (i.e. “microwalk”) ofthe original gapmers from Tables 57, 58, and 59. Gapmers were alsocreated with 4-9-4 MOE or 5-9-5 MOE motifs, and with constrained6(S)—CH₃-bicyclic nucleic acid (BNA) molecules at various nucleotidepositions.

These gapmers were tested in the GM04281 and GM02171 cell lines.Cultured GM04281 or GM02171 cells at a density of 25,000 cells per wellwere transfected using electroporation with 2,500 nM or 5,000 nMantisense oligonucleotide. After a treatment period of approximately 24hours, RNA was isolated from the cells and HTT mRNA levels were measuredby quantitative real-time PCR. HTT mRNA levels were adjusted accordingto total RNA content, as measured by RIBOGREEN. Results are presented aspercent inhibition of HTT mRNA, relative to untreated control cells.

The chimeric antisense oligonucleotides in Tables 60, 61, and 62 weredesigned as 3-9-3, 4-9-4, or 5-9-5 MOE gapmers. The parent gapmers, ISIS435890, ISIS 460210, ISIS 435879, ISIS 460209, ISIS 435870, and ISIS460207, from which the newly designed gapmers were derived are markedwith an asterisk (*) in the table. ISIS 387916 was included in the studyas a benchmark oligonucleotide against which the potency of theantisense oligonucleotides targeting nucleotides overlapping each SNPposition could be compared.

The 3-9-3 gapmers are 15 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 3 nucleotides each.

The 4-9-4 gapmers are 17 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 4 nucleotides each.

The 5-9-5 gapmers are 19 nucleotides in length, wherein the central gapsegment is comprised of nine 2′-deoxynucleotides and is flanked on both5′ and 3′ directions by wings comprising 5 nucleotides each.

The internucleoside linkages throughout each gapmer are phosphorothioate(P═S) linkages. All cytosine nucleobases throughout each gapmer are5-methylcytosines. Bolded and underlined nucleotides in Tables 60, 61,and 62 indicate the positions of the 6(S)—CH₃—BNA (e.g. cEtmolecules)molecules in each gapmer. Italicized nucleotides are MOEsubunits.

The gapmers are organized in Tables 60, 61, and 62, according to the SNPsite they target. “Start site” indicates the 5′-most nucleotide to whichthe gapmer is targeted. “Stop site” indicates the 3′-most nucleotide towhich the gapmer is targeted. ‘Target allele’ indicates whether thegapmer is targeted to the major or the minor allele. The number inparentheses indicates the position on the oligonucleotide opposite tothe SNP position.

TABLE 60 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs2298969(nucleobases 125888 to 125907 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Concentration in in ID position position ISIS No.Sequence Motif (nM) GM04281 GM02171 NO 145466 145485 387916 TCTCTATTGCA5-10-5 5000 57 24 6 CATTCCAAG 125888 125907 *435890 AAGGGATGCTG 5-9-52500 22 0 94 ACTTGGGC 5000 41 23 125890 125904 *460210 G GG ATGCTGAC3-9-3 2500 59 24 189 T TG G 5000 81 33 125889 125905 474870 A GGG ATGCTG4-9-4 2500 23 3 187 ACT TGG G 5000 44 34 125889 125905 474890 A G GGATGCTG 4-9-4 2500 38 6 187 ACT TG G G 5000 49 25 125889 125905 474910 AGG G ATGCTGA 4-9-4 2500 34 8 187 CT T GG G 5000 49 41 125889 125905474914 A GG G ATGCTGA 4-9-4 2500 44 14 187 CT T GG G 5000 44 21 125888125907 474918 A A G G G ATGCT 5-9-5 2500 31 0 94 GACT T G G G C 5000 2625 125888 125907 474922 A A G G G ATGCT 5-9-5 2500 33 14 94 GACT T G G GC 5000 65 24 125889 125905 476332 A G G G ATGCTG 4-9-4 2500 23 13 187ACT T G G G 5000 51 42 125888 125907 476336 A A G G GATGCTG 5-9-5 2500 50 94 ACTTGG G C 5000 43 9

TABLE 61 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs7685686(nucleobases 146786 to 146805 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Concentration in in ID position position ISIS No.Sequence Motif (nM) GM04281 GM02171 NO 145466 145485 387916 TCTCTATTGCA5-10-5 5000 57 24 6 CATTCCAAG 146786 146805 *435879 AATAAATTGTC 5-9-52500 39 0 99 ATCACCAG 5000 59 19 146788 146802 *460209 T AA ATTGTCAT3-9-3 2500 3 0 203 C AC C 5000 13 5 146787 146803 474871 A TAA ATTGTCA4-9-4 2500 82 32 200 TC ACC A 5000 83 58 146787 146803 474891 A T AAATTGTCA 4-9-4 2500 84 29 200 TC AC C A 5000 89 56 146787 146803 474911 ATA A ATTGTCA 4-9-4 2500 70 18 200 TC A CC A 5000 83 40 146787 146803474915 A TA A ATTGTCA 4-9-4 2500 38 9 200 TC A CC A 5000 74 14 146786146805 474919 A A T A A ATTGTC 5-9-5 2500 80 7 99 ATC A C C A G 5000 8437 146786 146805 474923 A A T A A ATTGTC 5-9-5 2500 74 32 99 ATC A C C AG 5000 83 51 146787 146803 476333 A T A A ATTGTCA 4-9-4 2500 75 28 200TC A C C A 5000 86 21 146786 146805 476337 A A T A AATTGTC 5-9-5 2500 716 99 ATCA C C A G 5000 83 31

TABLE 62 Comparison of inhibition of human HTT mRNA levels by ISIS387916 and chimeric antisense oligonucleotides targeted to SNP rs362331(nucleobases 155478 to 155498 of SEQ ID NO: 1) % % inhibition inhibitionSEQ Start Stop Concentration in in ID position position ISIS No.Sequence Motif (nM) GM04281 GM02171 NO 145466 145485 387916 TCTCTATTGCAC5-10-5 5000 57 24 6 ATTCCAAG 155479 155498 *435870 GCACACAGTAG 5-9-52500 19 1 103 ATGAGGGA 5000 49 34 155481 155495 *460207 A CA CAGTAGAT3-9-3 2500 0 0 217 G AG G 5000 7 8 155480 155496 474872 C ACA CAGTAGA4-9-4 2500 35 9 214 TG AGG G 5000 63 37 155480 155496 474892 C A CACAGTAGA 4-9-4 2500 43 16 214 TG AG G G 5000 69 31 155480 155496 474912 CAC A CAGTAGA 4-9-4 2500 16 9 214 TG A GG G 5000 36 6 155480 155496474916 C AC A CAGTAGA 4-9-4 2500 22 5 214 TG A GG G 5000 47 7 155479155498 474920 G C A C A CAGTAG 5-9-5 2500 19 0 103 ATG A G G G A 5000 4323 155479 155498 474924 G C A C A CAGTAG 5-9-5 2500 29 8 103 ATG A G G GA 5000 48 22 155480 155496 476334 C A C A CAGTAGA 4-9-4 2500 35 7 214 TG A G G G 5000 62 32 155479 155498 476338 G C A C ACAGTAG 5-9-5 2500 269 103 ATGA G G G A 5000 40 4 155479 155495 474873 A CAC AGTAGAT 4-9-42500 53 9 285 GA GGG A 5000 61 29 155479 155495 474893 A C AC AGTAGAT4-9-4 2500 47 5 285 GA GG G A 5000 59 30 155479 155495 474913 A CA CAGTAGAT 4-9-4 2500 30 16 285 GA G GG A 5000 29 17 155479 155495 474917 ACA C AGTAGAT 4-9-4 2500 23 12 285 GA G GG A 5000 40 5 155478 155497474921 C A C A C AGTAGA 5-9-5 2500 28 0 212 TGA G G G A G 5000 43 23155478 155497 474925 C A C A C AGTAGA 5-9-5 2500 30 9 212 TGA G G G A G5000 61 34 155479 155495 476335 A C A C AGTAGAT 4-9-4 2500 35 2 285 GA GG G A 5000 53 31 155478 155497 476339 C A C A CAGTAGA 5-9-5 2500 15 0212 TGAG G G A G 5000 34 13

Example 12 Dose-Dependent Antisense Inhibition of Human Huntingtin mRNALevels in Coriell Fibroblast Cell Lines

Gapmers from the studies described in Example 11 were selected andtested at various doses in GM04281, GM02171 and GM02173B cell lines.Each cell line was plated at a density of 25,000 cells per well andtransfected using electroporation with 625 nM, 1,250 nM, 2,500 nM, 5,000nM and 10,000 nM concentrations of antisense oligonucleotide, asspecified in Tables 63, 64, and 65. After a treatment period ofapproximately 16 hours, RNA was isolated from the cells and HTT mRNAlevels were measured by quantitative real-time PCR. Human HTT primerprobe set RTS2617 was used to measure mRNA levels. HTT mRNA levels wereadjusted according to total RNA content, as measured by RIBOGREEN.Results are presented as percent inhibition of HTT mRNA, relative tountreated control cells. IC₅₀ values are also provided in Tables 63, 64,and 65.

TABLE 63 Dose-dependent antisense inhibition of human HTT in GM04281cells ISIS 625 1250 2500 5000 10000 IC₅₀ No nM nM nM nM nM (μM) 38791670 83 94 96 98 <0.6 460207 51 63 83 91 93 0.5 460209 83 93 96 97 97 <0.6460210 70 89 94 97 98 0.6 474871 94 97 96 96 95 <0.6 474873 51 73 89 9495 0.5 474891 93 95 97 96 95 <0.6 474892 48 72 89 93 95 0.6 474911 85 9296 95 94 <0.6 474919 89 94 95 94 96 <0.6 474922 21 47 73 86 96 1.5474923 86 94 96 95 94 <0.6 476333 92 94 95 95 96 <0.6 476334 45 70 87 9295 0.6 476337 83 92 95 96 96 <0.6

TABLE 64 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 625 1250 2500 5000 10000 IC₅₀ No nM nM nM nM nM (μM) 38791628 38 63 82 99 1.6 460207 16 0 20 22 55 10.0 460209 27 50 61 87 94 9.9460210 34 60 80 86 97 0.9 474871 62 74 84 87 90 0.1 474873 13 29 61 7789 2.2 474891 57 72 80 83 88 0.2 474892 23 26 51 68 81 2.5 474911 47 5868 72 82 0.7 474919 44 48 65 71 83 1.1 474922 15 27 49 74 79 2.6 47492327 53 74 79 84 1.5 476333 42 53 75 76 84 1.0 476334 20 23 58 71 87 2.3476337 23 34 60 62 75 2.7

TABLE 65 Dose-dependent antisense inhibition of human HTT in GM02173Bcells ISIS 625 1250 2500 5000 10000 IC₅₀ No nM nM nM nM nM (μM) 38791638 75 89 95 99 0.9 460207 13 27 52 46 63 6.5 460209 79 68 84 90 92 <0.6460210 37 62 79 92 97 0.9 474871 74 83 87 92 89 <0.6 474873 22 32 67 7292 1.9 474891 69 78 84 89 89 <0.6 474892 26 50 75 83 91 1.3 474911 50 6676 86 86 0.6 474919 57 67 74 87 82 <0.6 474922 15 32 61 71 90 2.2 47492349 67 78 83 85 0.5 476333 58 71 78 87 89 <0.6 476334 20 42 63 76 91 1.8476337 48 63 71 79 80 0.6

Example 13 Strategy for Selection of Antisense Oligonucleotides Based onPotency and Selectivity

Gapmers from each of the studies described above were selected forfurther analysis based on potency and selectivity.

Potency was based on the percent inhibition of HTT mRNA achieved by theantisense oligonucleotides targeting a SNP compared to the percentinhibition of HTT mRNA achieved by the benchmark oligonucleotide, ISIS387916.

Selectivity was based on the ability of the antisense oligonucleotidestargeting a SNP to inhibit expression of the major allele and not of theminor allele. The usage of the three cell lines with different genotypesat each SNP position facilitated this process.

ISIS 460065 (5′-ATAAATTGTCATCACCAG-3′ (SEQ ID NO: 199)) is a 4-9-5 MOEgapmer targeted to SNP rs7685686 (major allele A, minor allele G) atposition 9 of the oligonucleotide. The GM04281 cell line is homozygousAA at SNP position rs7685686. The GM02173B cell line is heterozygous AGat SNP position rs7685686. The GM02171 cell line is homozygous GG at SNPposition rs7685686. Therefore, selectivity is shown if ISIS 460065causes potent inhibition of HTT mRNA in GM04281, less potent inhibitionof HTT mRNA in GM02173, and little to no significant inhibition of HTTmRNA in GM02171. IC50 values taken from Table 20, 21, and 22, andpresented below in Table 66, confirm varying degrees of inhibition inthe three cell lines, wherein expression was most reduced in thehomozygous AA cell line, moderately reduced in the heterozygous AG cellline, and less reduced in the homozygous GG cell line. IC50 is theconcentration of antisense oligonucleotide required for 50 percentinhibition HTT mRNA. IC50 values are in μM.

TABLE 66 Genotype of the Coriell cell lines for SNP rs7685686 andcomparison of inhibition of HTT mRNA by ISIS 460065 in each cell lineGM04281 GM02173B GM02171 Genotype AA AG GG IC₅₀ with ISIS 1.1 3.6 10.3460065

ISIS 459978 (5′-ACAGTGCTACCCAACCT-3′ (SEQ ID NO: 174)) is a 2-9-6 MOEgapmer targeted to SNP rs4690072 (major allele T, minor allele G) atposition 9 of the oligonucleotide. The GM04281 cell line is homozygousTT at SNP position rs4690072. The GM02173B cell line is heterozygous TGat SNP position rs4690072. The GM02171 cell line is homozygous GG at SNPposition rs4690072. Therefore, selectivity is shown if ISIS 459978causes potent inhibition of HTT mRNA in GM04281, less potent inhibitionof HTT mRNA in GM02173, and little to no significant inhibition of HTTmRNA in GM02171. IC50 values taken from Table 20, 21, and 22, andpresented below in Table 67, confirm varying degrees of inhibition inthe three cell lines, wherein expression was most reduced in thehomozygous TT cell line, moderately reduced in the heterozygous TG cellline, and less reduced in the homozygous GG cell line. IC50 is theconcentration of antisense oligonucleotide required for 50 percentinhibition HTT mRNA. IC50 values are in μM.

TABLE 67 Genotype of the Coriell cell lines for SNP rs4690072 andcomparison of inhibition of HTT mRNA by ISIS 459978 in each cell lineGM04281 GM02173B GM02171 Genotype TT TG GG IC₅₀ with ISIS 2.5 8.4 12.7459978

ISIS 460028 (5′-GAGCAGCTGCAACCTGGCA-3′ (SEQ ID NO: 149)) is a 4-11-4 MOEgapmer targeted to SNP rs362306 (major allele G, minor allele A) atposition 10 of the oligonucleotide. The GM04281 cell line is homozygousGG at SNP position rs362306. The GM02173B and GM02171 cell lines areheterozygous GA at SNP position rs362306. Therefore, selectivity isshown if ISIS 460028 causes potent inhibition of HTT mRNA in GM04281 andless potent inhibition of HTT mRNA in GM02173 and GM02171. IC50 valuestaken from Table 20, 21, and 22, and presented below in Table 68,confirm varying degrees of inhibition between the GM04281 cell line andthe GM02173B and GM02171 cell lines, wherein expression was most reducedin the homozygous GG cell line and less reduced in the heterozygous AGcell line. IC50 is the concentration of antisense oligonucleotiderequired for 50 percent inhibition HTT mRNA. IC50 values are in μM.

TABLE 68 Genotype of the Coriell cell lines for SNP rs362306 andcomparison of inhibition of HTT mRNA by ISIS 460028 in each cell lineGM04281 GM02173B GM02171 Genotype GG AG AG IC₅₀ with ISIS 1.4 5.2 5.3460028

Example 14 Strategy for Selection of Antisense Oligonucleotides with cEtMotifs Based on Potency and Selectivity

Gapmers from each of the studies described above were selected forfurther analysis based on potency and selectivity.

Potency was based on the percent inhibition of HTT mRNA achieved by theantisense oligonucleotides targeting a SNP compared to the percentinhibition of HTT mRNA achieved by the benchmark oligonucleotide, ISIS387916.

Selectivity was based on the ability of the antisense oligonucleotidestargeting a SNP to inhibit expression of the major allele and not of theminor allele. The usage of the three cell lines with different genotypesat each SNP position facilitated this process.

ISIS 460209 (5′-TAAATTGTCATCACC-3′ (SEQ ID NO: 203)) is a 3-9-3 gapmerwith cEt subunits at positions 2, 3, 13, and 14, targeted to SNPrs7685686 (major allele A, minor allele G) at position 8 of theoligonucleotide. The GM04281 cell line is homozygous AA at SNP positionrs7685686. The GM02173B cell line is heterozygous AG at SNP positionrs7685686. The GM02171 cell line is homozygous GG at SNP positionrs7685686. Therefore, selectivity is shown if ISIS 460209 causes potentinhibition of HTT mRNA in GM04281, less potent inhibition of HTT mRNA inGM02173, and little to no significant inhibition of HTT mRNA in GM02171.IC₅₀ values taken from Table 57, 58, and 59, and presented below inTable 69, confirm varying degrees of inhibition in the three cell lines,wherein expression was most reduced in the homozygous AA cell line,moderately reduced in the heterozygous AG cell line, and less reduced inthe homozygous GG cell line. IC₅₀ is the concentration of antisenseoligonucleotide required for 50 percent inhibition HTT mRNA. IC₅₀ valuesare in μM.

TABLE 69 Genotype of the Coriell cell lines for SNP rs7685686 andcomparison of inhibition of HTT mRNA by ISIS 460209 in each cell lineGM04281 GM02173B GM02171 Genotype AA AG GG IC₅₀ with ISIS 0.2 0.8 1.6460209

ISIS 460208 (5′-CAGTGCTACCCAACC-3′ (SEQ ID NO: 177)) is a 3-9-3 gapmerwith cEt subunits at positions 2, 3, 13, and 14, targeted to SNPrs4690072 (major allele T, minor allele G) at position 8 of theoligonucleotide. The GM04281 cell line is homozygous TT at SNP positionrs4690072. The GM02173B cell line is heterozygous TG at SNP positionrs4690072. The GM02171 cell line is homozygous GG at SNP positionrs4690072. Therefore, selectivity is shown if ISIS 460208 causes potentinhibition of HTT mRNA in GM04281, less potent inhibition of HTT mRNA inGM02173, and little to no significant inhibition of HTT mRNA in GM02171.IC₅₀ values taken from Table 57, 58, and 59, and presented below inTable 70, confirm varying degrees of inhibition in the three cell lines,wherein expression was most reduced in the homozygous TT cell line,moderately reduced in the heterozygous TG cell line, and less reduced inthe homozygous GG cell line. IC₅₀ is the concentration of antisenseoligonucleotide required for 50 percent inhibition HTT mRNA. IC₅₀ valuesare in μM.

TABLE 70 Genotype of the Coriell cell lines for SNP rs4690072 andcomparison of inhibition of HTT mRNA by ISIS 460208 in each cell lineGM04281 GM02173B GM02171 Genotype TT TG GG IC₅₀ with ISIS 1.5 9.0 10.8460208

ISIS 460206 (5′-GCAGCTGCAACCTGG-3′ (SEQ ID NO: 231)) is a 3-9-3 gapmerwith cEt subunits at positions 2, 3, 13, and 14, targeted to SNPrs362306 (major allele G, minor allele A) at position 8 of theoligonucleotide. The GM04281 cell line is homozygous GG at SNP positionrs362306. The GM02173B and GM02171 cell lines are heterozygous GA at SNPposition rs362306. Therefore, selectivity is shown if ISIS 460206 causespotent inhibition of HTT mRNA in GM04281 and less potent inhibition ofHTT mRNA in GM02173 and GM02171. IC₅₀ values taken from Table 57, 58,and 59, and presented below in Table 71, confirm varying degrees ofinhibition between the GM04281 cell line and the GM02173B and GM02171cell lines, wherein expression was most reduced in the homozygous GGcell line and less reduced in the heterozygous AG cell line. IC₅₀ is theconcentration of antisense oligonucleotide required for 50 percentinhibition HTT mRNA. IC₅₀ values are in μM.

TABLE 71 Genotype of the Coriellcell lines for SNP rs362306 andcomparison of inhibition of HTT mRNA by ISIS 460206 in each cell lineGM04281 GM02173B GM02171 Genotype GG AG AG IC₅₀ with ISIS 2.3 2.7 2.7460206

Example 15 Comparison of SNPs in Various Cell Lines and Mouse ModelsAssociated with Huntington's Disease

The genotype at various SNP positions associated with Huntington'sdisease was compared amongst the three Corriell cell lines, used in theabove Examples, as well as with the GM04022 fibroblast, the BACHD mousemodel and the YAC18 mouse model.

The donor patient of the GM04022 fibroblast cell line was heterozygousat SNP position rs363125 (NCBI Entrez SNP database), harboring an Aallele (adenine) and a C allele (cytosine) at nucleotide 5310 of SEQ IDNO: 2 (van Bilsen, P. H. J. et al., Human Gene Therapy. 19: 710-718,2008). YAC18 mice were developed with a YAC transgene containing humanhuntingtin gene (Hodgson, et al. Hum. Mol. Genet. 5: 1875-85, 1996).BACHD mice were developed expressing a full-length mutant huntingtingene with 97 glutamine repeats under the control of a bacterialartificial chromosome (Gray, M. et al., J. Neurosc. 28: 6182-95, 2008).The comparative genotype at the indicated SNP positions in all four celllines and mouse models is presented in Table 72.

TABLE 72 Genotypes of the Coriell cell lines and Huntington mouse modelsSNP GM02171 GM02173 GM04281 GM04022   BACHD YAC18   rs3856973 AA AG GGAG GG AA rs2285086 GG AG AA AG AA GG rs7659144 CG CG CC CG CC GGrs16843804 TC TC CC CC CC TT rs2024115 GG AG AA AG AA GG rs3733217 CC CCCC CC CC CC rs10015979 AA AG GG AA AA AA rs7691627 AA AG GG AG GG AArs2798235 GG GG GG AG GG GG rs4690072 GG TG TT TG TT GG rs6446723 CC TCTT TC TT CC rs363081 GG GG GG GG GG GG rs363080 CC CC CC TC CC CCrs363075 GG GG GG GG GG GG rs363064 TC TC CC CC CC TT rs3025849 AA AA AAAA AA AA rs363102 AA AA AA AG AA AA rs11731237 CC TC TT CC CC CCrs4690073 AA AG GG AG GG AA rs363144 TT TT TT TT TT TT rs3025838 CC CCCC CC CC CC rs34315806 TC TC CC CC CC TT rs363099 TC TC CC CC CC TTrs363096 CC TC TT CC TT CC rs2298967 TC TC TT TT TT CC rs2298969 GG AGAA AG AA GG rs6844859 CC TC TT TC TT CC rs363092 AA AC CC AC AA AArs7685686 GG AG AA AG AA GG rs363088 TA TA AA AA AA TT rs362331 CC TC TTTC TT CC rs916171 GG GC CC GC CC GG rs362322 AA AA AA AA AA AA rs362275TC TC CC CC CC TT rs362273 AG AG AA AA AA GG rs2276881 GG GG GG GG GG GGrs3121419 TC TC CC CC CC TT rs362272 — AG GG GG GG AA rs362271 AG AG GGGG GG AA rs3775061 AG AG AA AA AA GG rs362310 TC CC CC TC CC CC rs362307CC TC CC CC CC CC rs362306 AG AG GG GG GG AA rs362303 TC CC CC TC CC CCrs362296 AC AC AC CC CC AA

Example 16 Allele-Specific Inhibition Measured in BacHD Cortical Neurons

Antisense oligonucleotides, ISIS 460209 (5′-TAAATTGTCATCACC-3′ (SEQ IDNO: 203)), targeting SNP rs7685686 of human HTT, and ISIS 387916(TCTCTATTGCACATTCCAAG (SEQ ID NO: 6)), and with no human or murine SNPtarget site, were tested for their effect on Htt protein levels invitro. ISIS 387916 is cross-reactive with murine Htt mRNA (GENBANKAccession No. NM_(—)010414.1, designated herein as SEQ ID NO: 286) attarget start site 5763 with one mismatch. ISIS 460209 is cross-reactivewith murine Htt mRNA at target start site 6866 with three mismatches.

Primary BacHD cortical neurons, which express human Htt and murine Htt,were isolated in the following way: Embryos were dissected fromE15.5-E17.5 pregnant females. Cortices were dissected into ice-colddivalent-free Hank's Balanced Salt Solution (Invitrogen, 14025-134). Thecortices were chopped into pieces and digested with 0.05% Trypsin-EDTA(Invitrogen, 25300-120) at 37° C. for 8 minutes. The digestion washalted by addition of complete neurobasal media (Invitrogen, 10888-022).Cells were resuspended in media and treated with DNAse I (Invitrogen,18047-019). After titration through a 100 ul pipette tip, cells areresuspended in neurobasal media with B27 supplement (Invitrogen,17504-044), and counted. 1.7×10⁵ cells/well were plated in 24-wellplates precoated with poly-D-lysine (BD Biosciences, 354210). Neuronswere fed with 200 μl neurobasal media with B27 on the second day invitro.

ISIS 460209 or ISIS 387916 was added to the supplementary media fed toneurons on division 2 at 0.7 μM, 1.4 μM or 1.5 μM final concentrations.Cells were harvested after 8 days with into 1 mL of media using a cellscraper. Cells were centrifuged at 2,500 rpm for 5 min at 4° C. and thepellets were resuspended in a buffer of 50 mM Tris, pH=8.0, 150 mM NaCl,1% Igepal, 40 mM β-glycerophosphate, 10 mM NaF, 1× Roche completeprotease inhibitor, 1 mM Sodium Orthovanadate and 800 μM PMSF. Thelysates were centrifuged after 15 min incubation and proteinconcentration was measured with the DC assay (BioRad).

Protein lysates were run on low-bis gels to separate huntingtin alleles(resolving gel—2001:Acrylamide:BIS (10% acrylamide, 0.5% BIS, 375 mMTrispH 8.8; stacking gel—4% Acrylamide-BIS(29:1), 156 mM Tris pH6.8; Runningbuffer—25 mM Tris, 190 mM Glycine, 0.1% SDS+10 μM beta-mercaptoethanoladded fresh). After electrophoresis, proteins in the gel weretransferred to a nitrocellulose membrane (Hybond-C Extra; GE HealthcareBio-Sciences) at 90V for 40′ to allow samples to penetrate the stackinggel and then at 190V for 2.5 h to resolve proteins.

Primary antibodies specific for human Htt and murine calnexin proteinwere used at 1:10,000 dilutions. HRP-conjugated anti-mouse secondaryantibody (1:10,000, Jackson ImmunoResearch Laboratories) was used forvisualizing proteins using SuperSignal West Pico ChemiluminescentSubstrate (Thermo Scientific). Protein bands were quantified usingImageJ software and normalized to calnexin levels. Protein bands werequantified using ImageJ software. Table 73 provides an estimate of thepercentage inhibition relative to the negative control sample. Thecomparative percent inhibitions of the human Htt protein and the murineHtt protein are presented.

TABLE 73 Effect of antisense inhibition on mutant human and wild-typemurine Htt protein (percent inhibition normalized to PBS control) Dose(μm) Human Murine ISIS 387916 0.7 54 38 1.4 75 58 1.5 92 88 ISIS 4602090.2 71 35 0.4 82 41 1.5 94 56

Example 17 Dose-Dependent Antisense Inhibition of Human Huntingtin mRNALevels in Coriell Fibroblast Cell Lines

Gapmers from the studies described in Examples, 3, 4, 10, and 12 wereselected and tested at various doses in GM04281, GM02171 and GM02173Bcell lines. Each cell line was plated at a density of 25,000 cells perwell and transfected using electroporation with 0.4747 nM, 1.5011 nM,4.7463 nM, 15.0079 nM 45.455 nM, 150.0527 nM, 474.4673 nM, 1,500.27 nM,4,743.833 nM, and 15,000 nM concentrations of antisense oligonucleotide,as specified in Tables 72, 73, and 74. After a treatment period ofapproximately 16 hours, RNA was isolated from the cells and HTT mRNAlevels were measured by quantitative real-time PCR. Human HTT primerprobe set RTS2617 was used to measure mRNA levels. HTT mRNA levels wereadjusted according to total RNA content, as measured by RIBOGREEN.Results are presented as percent inhibition of HTT mRNA, relative tountreated control cells. IC₅₀ values are also provided in Tables 72, 73,and 74.

TABLE 74 Dose-dependent antisense inhibition of human HTT in GM04281cells ISIS 0.4747 1.5011 4.7463 15.0079 47.455 150.0527 474.4673 1500.274743.833 15000.0 IC₅₀ No nM nM nM nM nM nM nM nM nM nM (μM) 387916 15 124 5 7 26 70 89 98 99 0.33 435879 0 8 19 13 24 23 45 53 84 93 0.25 43589016 1 8 12 25 23 32 52 61 91 0.82 460209 2 9 21 17 36 46 80 89 94 93 0.09460210 4 7 5 19 20 35 69 85 98 98 0.21 476333 7 10 8 11 42 65 86 93 9395 0.05

TABLE 75 Dose-dependent antisense inhibition of human HTT in GM02171cells ISIS 0.4747 1.5011 4.7463 15.0079 47.455 150.0527 474.4673 1500.274743.833 15000.0 IC₅₀ No nM nM nM nM nM nM nM nM nM nM (μM) 387916 22 80 9 0 32 60 90 96 97 0.27 435879 0 1 6 2 0 0 8 9 46 57 7.62 435890 0 0 06 0 0 0 31 27 71 4.37 460209 11 5 15 0 0 7 30 69 82 88 0.96 460210 0 0 02 17 18 38 70 93 95 0.56 476333 0 0 0 0 13 18 44 69 72 91 0.75

TABLE 76 Dose-dependent antisense inhibition of human HTT in GM02173Bcells ISIS 0.4747 1.5011 4.7463 15.0079 47.455 150.0527 474.4673 1500.274743.833 15000.0 IC₅₀ No nM nM nM nM nM nM nM nM nM nM (μM) 387916 3 177 25 27 33 65 88 98 99 0.19 435879 0 6 0 8 3 10 16 24 50 68 3.72 4358900 13 0 1 2 12 16 23 49 82 4.60 460209 0 7 29 2 9 32 52 71 82 86 0.27460210 0 13 0 5 16 18 49 74 93 97 0.27 476333 11 13 20 7 23 36 63 75 8390 0.13

Example 18 Validation of the Specificity of ISIS OligonucleotidesTargeting SNPs of Human Huntingtin by the Molecular Beacon Assay

Some of the gapmers from the study described in Example 17 were testedin GM04022 fibroblasts (from the Coriell Institute for MedicalResearch).

To verify allele-specific suppression of HTT mRNA in GM04022 fibroblastsby ISIS 435879, ISIS 460209, and ISIS 476333, the Molecular Beaconassay, as described in the van Bilsen at el publication (van Bilsen, P.H. J. et al., Human Gene Therapy. 19: 710-718, 2008), was conductedusing ‘molecular beacon’ synthetic oligonucleotides linked with afluorophore and quencher. GM04022 fibroblasts were transfected byelectroporation with ISIS 435879, ISIS 460209, or ISIS 476333 at 0.06μM, 0.19 μM, 0.56 μM, 1.67 μM, 5 μM and 15 μM concentrations ofantisense oligonucleotide, as specified in Tables 75-77. ISIS 387916 wasincluded in the assay as a benchmark oligonucleotide. The qRT-PCR assayfor molecular beacon for the A allele was conducted with the annealingtemperature at 56.5° C. The qRT-PCR assay for molecular beacon for the Callele was conducted with the annealing temperature at 62.0° C. Primerprobe set RTS2617 was used to measure the total HTT mRNA reduction. Theresults of the assay are presented in Tables 77-79 as percent inhibitionover the PBS control. The results demonstrate that the SNP-specific ISISoligonucleotides specifically target the C allele of rs7685686 comparedto the A allele (Table 80).

TABLE 77 Dose-dependent antisense inhibition of the A allele ofrs7685686 in GM04022 fibroblasts ISIS 0.06 0.19 0.56 1.67 5.00 15.00IC₅₀ No μM μM μM μM μM μM (μM) 387916 33 40 53 90 99 98 0.56 435879 0 050 29 38 47 10.8 460209 14 4 54 73 81 95 0.53 476333 2 44 41 77 91 860.64

TABLE 78 Dose-dependent antisense inhibition of the C allele ofrs7685686 in GM04022 fibroblasts ISIS 0.06 0.19 0.56 1.67 5.00 15.00IC₅₀ No μM μM μM μM μM μM (μM) 387916 41 42 46 86 95 92 0.54 435879 0 075 60 68 81 2.9 460209 35 48 76 84 88 92 0.19 476333 22 60 75 84 90 930.15

TABLE 79 Dose-dependent antisense inhibition of total HTT mRNA inGM04022 fibroblasts ISIS 0.06 0.19 0.56 1.67 5.00 15.00 No μM μM μM μMμM μM 387916 32 59 49 89 98 99 435879 0 0 42 25 41 62 460209 26 27 54 7584 96 476333 25 51 58 82 92 90

TABLE 80 IC₅₀ ratio (A/C) in GM04022 fibroblasts ISIS No Ratio 3879161.0 435879 4.2 460209 2.8 476333 4.3

Example 19 Allele-Specific Inhibition Measured in Cortical Neurons fromBACHD and YAC18 Mice

In order to identify potential SNPs for screening of humanallele-specific ISIS oligonucleotides, the HTT mRNA of YAC18 and BACHDmice were sequenced by the Goldengate 96SNP assay. It was determinedthat the BAC and YAC mice carried different alleles at several key SNPpositions (Table 72) and could therefore be used as a screening tool forallele-specific knockdown. Each of the SNP positions chosen fortargeting in the mouse strains were also compared to human HDchromosomes. For each target, approximately 50% of the human HDpopulation is heterozygous for the target expressed in the BACHD mice,but not the YAC18 mice.

In order to verify the allele-specificity of the ISIS oligonucleotides(described in Examples 2, 9, 17 and 18), the antisense oligonucleotides,ISIS 460207, targeting SNP rs362331; ISIS 460209, targeting SNPrs7685686; ISIS 435879, targeting SNP rs7685686; ISIS 476333, targetingSNP rs7685686; ISIS 460210, targeting SNP rs2298969; ISIS 435874,targeting SNP rs4690072; ISIS 460208, targeting SNP rs4690072; ISIS435331, targeting SNP rs2024115; and ISIS 435871, targeting SNPrs363088, were tested for their effect on HTT protein levels in BACHDand YAC18 cortical neurons. ISIS 387916, which has no human or murineSNP target site, was used as the benchmark. ISIS 387916 iscross-reactive with murine HTT mRNA (GENBANK Accession No.NM_(—)010414.1, designated herein as SEQ ID NO: 286) at target startsite 5763 with one mismatch. It was expected that treatment with theallele-specific antisense oligonucleotides would cause significantinhibition of HTT mRNA in the BACHD neurons and not in the YAC18neurons. It was also expected that treatment with ISIS 387916 wouldcause inhibition of HTT mRNA in both sets of neurons.

YAC18 cultures were prepared from E16.5 pregnant female YAC18 (line 60,+/+) mice who had been bred with YAC18 (line 60, +/+) males. All progenyare thus homozygous YAC18 (line 60), facilitating pooled corticalcultures. BACHD E16.5 embryos were isolated from pregnant BACHD (+/−)mice who had been bred with pregnant BACHD (+/−) male mice,necessitating single pup cultures and genotyping. Single cortices wereisolated, using caution to prevent cross-contamination of samples. Eachdissociated cortex was used to seed 5 wells of a 6-well plate. Aftergenotyping, only BACHD (+/−) cultures were used for ASO treatment. Theantisense oligonucleotides were added to the supplementary media fed tothe neurons on division 2. Cells were harvested after 8 days with into 1mL of media using a cell scraper. Cells were centrifuged at 2,500 rpmfor 5 min at 4° C. and the pellets were resuspended in a buffer of 50 mMTris, pH=8.0, 150 mM NaCl, 1% Igepal, 40 mM β-glycerophosphate, 10 mMNaF, 1× Roche complete protease inhibitor, 1 mM Sodium Orthovanadate and800 μM PMSF. The lysates were centrifuged after 15 min incubation andprotein concentration was measured with the DC assay (BioRad).

Protein lysates were run on low-bis gels to separate huntingtin alleles(resolving gel—2001:Acrylamide:BIS (10% acrylamide, 0.5% BIS, 375 mMTrispH 8.8; stacking gel—4% Acrylamide-BIS(29:1), 156 mM Tris pH6.8; Runningbuffer—25 mM Tris, 190 mM Glycine, 0.1% SDS+10 μM beta-mercaptoethanoladded fresh). After electrophoresis, proteins in the gel weretransferred to a nitrocellulose membrane (Hybond-C Extra; GE HealthcareBio-Sciences) at 90V for 40′ to allow samples to penetrate the stackinggel and then at 190V for 2.5 h to resolve proteins.

Primary antibodies specific for human HTT and murine calnexin proteinwere used at 1:10,000 dilutions. HRP-conjugated anti-mouse secondaryantibody (1:10,000, Jackson ImmunoResearch Laboratories) was used forvisualizing proteins using SuperSignal West Pico ChemiluminescentSubstrate (Thermo Scientific). Protein bands were quantified usingImageJ software and normalized to calnexin levels. Tables 81-91 providethe percentage inhibition relative to the untreated control sample. Thepercentage inhibition of human HTT protein levels in BACHD and YAC18neurons are presented.

TABLE 81 HTT SNPs in BACHD and YAC18 mice and correlation with human HTTSNPs Allele present in % of Allele Allele human human present presentpatients patients in in with high heterozgous YAC18 BACHD CAG at the SNPSNP Mice Mice repeats position rs2024115 G A A 48 rs2298969 G A A 52rs362331 C T T 49 rs363088 G T T 38 rs4690072 T A A 49 rs7685686 G A A49

TABLE 82 Effect of antisense inhibition by ISIS 387916 in BACHD andYAC18 neurons 500 nM 1500 nM YAC18 69 81 BACHD 84 90

TABLE 83 Effect of antisense inhibition by ISIS 435331, targetingrs2024115 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 0 0 BACHD 3943

TABLE 84 Effect of antisense inhibition by ISIS 460210, targetingrs2298969 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 31 51 BACHD 7989

TABLE 85 Effect of antisense inhibition by ISIS 460207, targetingrs362331 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 0 0 BACHD 29 44

TABLE 86 Effect of antisense inhibition by ISIS 435871, targetingrs363088 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 0 0 BACHD 51 68

TABLE 87 Effect of antisense inhibition by ISIS 435874, targetingrs4690072 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 9 5 BACHD 3044

TABLE 88 Effect of antisense inhibition by ISIS 460208, targetingrs4690072 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 1 8 BACHD 5468

TABLE 89 Effect of antisense inhibition by ISIS 460209, targetingrs7685686 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 12 32 BACHD 7283

TABLE 90 Effect of antisense inhibition by ISIS 435879, targetingrs7685686 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 0 7 BACHD 3658

TABLE 91 Effect of antisense inhibition by ISIS 476333, targetingrs7685686 in BACHD and YAC18 neurons 500 nM 1500 nM YAC18 46 61 BACHD 8991

What is claimed is: 1.-97. (canceled)
 98. A method for determiningwhether an agent selectively inhibits expression of a first allelicvariant of a gene relative to a second allelic variant of said genewherein said first allelic variant comprises a first nucleotide at a SNPposition and said second allelic variant comprises a second nucleotideat said SNP position, comprising: contacting a first cell, tissue oranimal with said agent at one or more concentrations, wherein said firstcell, tissue or animal is homozygous for said first nucleotide at saidSNP position; contacting a second cell, tissue or animal with said agentat one or more concentrations, wherein said second cell, tissue oranimal is homozygous for said second nucleotide at said SNP position oris heterozygous for said first and said second nucleotides at said SNPposition; measuring inhibition of expression of said allelic variant ineach of said cell, tissue or animal for each of said one or moreconcentrations of said agent; and comparing said inhibition ofexpression in each of said cell, tissue or animal for each of said oneor more concentrations of said agent to determine whether said agentselectively inhibits expression of said first allelic variant of saidgene relative to said second allelic variant of said gene.
 99. Themethod of claim 98, further comprising the step of contacting a thirdcell, tissue, or animal with said agent at one or more concentrations,wherein said third cell, tissue, or animal is homozygous for said secondnucleotide at said SNP position or is heterozygous for said first andsaid second nucleotides at said SNP position and has a differentgenotype than said first cell, tissue, or animal and said second cell,tissue, or animal.
 100. The method of claim 98, wherein said firstallelic variant is a mutant allele and said second allelic variant is awild-type allele.
 101. The method of claim 98, wherein said firstallelic variant is a wild-type allele and said second allelic variant isa mutant allele.
 102. The method of claim 101, wherein said mutantallelic variant is associated with disease.
 103. The method of claim102, wherein said disease is Huntington's Disease.
 104. The method ofclaim 98, wherein said first nucleotide is in linkage disequilibriumwith a disease associated mutation.
 105. The method of claim 98, whereinsaid second nucleotide is in linkage disequilibrium with a diseaseassociated mutation.
 106. The method of claim 105, wherein said diseaseassociated mutation is a tri-nucleotide repeat expansion.
 107. Themethod of claim 106, wherein said tri-nucleotide repeat expansion is aCAG expansion.
 108. The method of claim 107, wherein said CAG expansionis in a HTT gene.
 109. The method of claim 98, wherein said firstnucleotide is A.
 110. The method of claim 109, wherein said secondnucleotide is any of C, G, or T.
 111. The method of claim 109, whereinsaid second nucleotide is any of A, G, or T.
 112. The method of claim98, wherein said agent is an antisense compound.
 113. The method ofclaim 112, said antisense compound is an antisense oligonucleotide. 114.The method of claim 113, wherein said antisense oligonucleotide is agapmer.
 115. The method of claim 113, wherein said gapmer has awing-gap-wing motif.
 116. The method of claim 115, wherein thewing-gap-wing motif is any one of the group consisting of 5-10-5, 2-9-6,3-9-3, 3-9-4, 3-9-5, 4-7-4, 4-9-3, 4-9-4, 4-9-5, 4-10-5, 4-11-4, 4-11-5,5-7-5, 5-8-6, 5-9-3, 5-9-5, 5-10-4, 5-10-5, 6-7-6, 6-8-5, and 6-9-2.117. The method of claim 98, wherein said SNP position is any of thegroup consisting of rs6446723, rs3856973, rs2285086, rs363092, rs916171,rs6844859, rs7691627, rs4690073, rs2024115, rs11731237, rs362296,rs10015979, rs7659144, rs363096, rs362273, rs16843804, rs362271,rs362275, rs3121419, rs362272, rs3775061, rs34315806, rs363099,rs2298967, rs363088, rs363064, rs363102, rs2798235, rs363080, rs363072,rs363125, rs362303, rs362310, rs10488840, rs362325, rs35892913,rs363102, rs363096, rs11731237, rs10015979, rs363080, rs2798235,rs1936032, rs2276881, rs363070, rs35892913, rs12502045, rs6446723,rs7685686, rs3733217, rs6844859, rs362331, rs1143646, rs2285086,rs2298969, rs4690072, rs916171, rs3025849, rs7691627, rs4690073,rs3856973, rs363092, rs362310, rs362325, rs363144, rs362303, rs34315806,rs363099, rs363081, rs3775061, rs2024115, rs10488840, rs363125,rs362296, rs2298967, rs363088, rs363064, rs362275, rs3121419, rs3025849,rs363070, rs362273, rs362272, rs362306, rs362271, rs363072, rs16843804,rs7659144, rs363120, and rs12502045.